Strain identifier

BacDive ID: 137887

Type strain: No

Species: Microbacterium binotii

Strain Designation: 75-77

Strain history: CIP <- 1986, Lab. Ident. Inst. Pasteur, Paris, France: strain 75-77 <- Inst. Pasteur, Lille, France: strain 10988, Brevibacterium sp., "Microbacterium sp."

NCBI tax ID(s): 462710 (species)

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General

@ref: 38836

BacDive-ID: 137887

keywords: Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Microbacterium binotii 75-77 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Microbacteriaceae.

NCBI tax id

  • NCBI tax id: 462710
  • Matching level: species

strain history

  • @ref: 38836
  • history: CIP <- 1986, Lab. Ident. Inst. Pasteur, Paris, France: strain 75-77 <- Inst. Pasteur, Lille, France: strain 10988, Brevibacterium sp., "Microbacterium sp."

doi: 10.13145/bacdive137887.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium binotii
  • full scientific name: Microbacterium binotii Clermont et al. 2009

@ref: 38836

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium binotii

strain designation: 75-77

type strain: no

Morphology

cell morphology

  • @ref: 38836
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 38836
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38836MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
38836CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
38836CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
38836positivegrowth30mesophilic
38836positivegrowth15-37
38836nogrowth41thermophilic
38836nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 38836
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
38836NaClpositivegrowth0-6 %
38836NaClnogrowth8 %
38836NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
38836citrate-carbon source16947
38836esculin+hydrolysis4853
38836hippurate+hydrolysis606565
38836nitrate-reduction17632
38836nitrite-reduction16301
38836nitrate-respiration17632

antibiotic resistance

  • @ref: 38836
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 38836
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3883615688acetoin-
3883617234glucose+

enzymes

@refvalueactivityec
38836oxidase-
38836beta-galactosidase-3.2.1.23
38836alcohol dehydrogenase-1.1.1.1
38836gelatinase-
38836amylase+
38836DNase+
38836caseinase-3.4.21.50
38836catalase+1.11.1.6
38836tween esterase+
38836gamma-glutamyltransferase-2.3.2.2
38836lecithinase-
38836lipase-
38836lysine decarboxylase-4.1.1.18
38836ornithine decarboxylase-4.1.1.17
38836phenylalanine ammonia-lyase-4.3.1.24
38836protease-
38836tryptophan deaminase-
38836urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38836-++--++---++++-+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
38836+/---+-+---++++----+-----++++++-++------+/-+------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
38836+++++--+-++++--++++-+++--------+-+++-------------+------+---+-----------+-----+++-----+------------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
38836LilleFranceFRAEurope
38836LilleFranceFRAEuropeHuman, Blood1977

Safety information

risk assessment

  • @ref: 38836
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 38836

culture collection no.: CIP 102116

straininfo link

  • @ref: 94700
  • straininfo: 69286

literature

  • topic: Phylogeny
  • Pubmed-ID: 19406785
  • title: Microbacterium binotii sp. nov., isolated from human blood.
  • authors: Clermont D, Diard S, Bouchier C, Vivier C, Bimet F, Motreff L, Welker M, Kallow W, Bizet C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.003160-0
  • year: 2009
  • mesh: Actinomycetales/chemistry/*classification/genetics/isolation & purification, Actinomycetales Infections/*microbiology, Bacterial Typing Techniques, Base Composition, Blood/*microbiology, DNA, Bacterial/analysis/genetics, Genes, rRNA, Genotype, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38836Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102116Collection of Institut Pasteur (CIP 102116)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
94700Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69286.1