Strain identifier

BacDive ID: 13786

Type strain: Yes

Species: Epibacterium mobile

Strain Designation: N-615G15

Strain history: CIP <- 2006, NBRC <- NITE <- MBIC01146 <- H. Izumida, Marine Biotechnol. Inst., Alcaligenes sp.: strain N-615G15

NCBI tax ID(s): 379347 (species)

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General

@ref: 16845

BacDive-ID: 13786

DSM-Number: 23403

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Epibacterium mobile N-615G15 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from marine slime.

NCBI tax id

  • NCBI tax id: 379347
  • Matching level: species

strain history

@refhistory
16845<- NBRC; NBRC 101030 <- NBRC, NITE (Natl. Inst. of Technol. and Evaluation), Japan <- H. Izumida, Marine Biotechnol. Inst., Kamaishi, Japan; MBIC 01146
120987CIP <- 2006, NBRC <- NITE <- MBIC01146 <- H. Izumida, Marine Biotechnol. Inst., Alcaligenes sp.: strain N-615G15

doi: 10.13145/bacdive13786.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Epibacterium
  • species: Epibacterium mobile
  • full scientific name: Epibacterium mobile (Muramatsu et al. 2007) Wirth and Whitman 2018
  • synonyms

    @refsynonym
    20215Tritonibacter mobilis
    20215Tritonibacter mobilis subsp. pelagius
    20215Ruegeria pelagia
    20215Ruegeria mobilis

@ref: 16845

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Epibacterium

species: Epibacterium mobile

full scientific name: Tritonibacter mobilis subsp. mobilis (Muramatsu et al. 2007) Hördt et al. 2020

strain designation: N-615G15

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31871negative01-02 µm0.6-0.8 µmrod-shapedyes
69480negative99.993
120987negativeoval-shapedyes

colony morphology

  • @ref: 120987

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16845BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
37467Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120987CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
16845positivegrowth28mesophilic
31871positivegrowth05-35
31871positiveoptimum25-30mesophilic
37467positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
31871positivegrowth05-11alkaliphile
31871positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31871aerobe
120987obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.991

halophily

@refsaltgrowthtested relationconcentration
31871NaClpositivegrowth0-10 %
31871NaClpositiveoptimum01-03 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3187115963ribitol+carbon source
3187122599arabinose+carbon source
3187118403L-arabitol+carbon source
3187128757fructose+carbon source
3187133984fucose+carbon source
3187129864mannitol+carbon source
3187130911sorbitol+carbon source
3187117151xylitol+carbon source
12098717632nitrate-reduction
12098716301nitrite-reduction

metabolite production

  • @ref: 120987
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31871catalase+1.11.1.6
31871cytochrome oxidase+1.9.3.1
120987oxidase+
120987alcohol dehydrogenase-1.1.1.1
120987catalase+1.11.1.6
120987lysine decarboxylase-4.1.1.18
120987ornithine decarboxylase-4.1.1.17
120987urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120987-+++-+++--++---+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16845marine slimeIshigaki IslandJapanJPNAsia
120987Environment, Slime, marine environmentOkinawaJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_1008.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_355;97_675;98_785;99_1008&stattab=map
  • Last taxonomy: Epibacterium mobile subclade
  • 16S sequence: AB255401
  • Sequence Identity:
  • Total samples: 19312
  • soil counts: 558
  • aquatic counts: 16540
  • animal counts: 2089
  • plant counts: 125

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
168451Risk group (German classification)
1209871Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Ruegeria mobilis strain NBRC 101030 16S ribosomal RNA gene, partial sequenceEU9771371425ena379347
16845Ruegeria mobilis gene for 16S ribosomal RNA, partial sequence, strain: NBRC 101030AB2554011387ena379347

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Tritonibacter mobilis DSM 23403GCA_900106635contigncbi379347
66792Tritonibacter mobilis NBRC101030GCA_001681715contigncbi379347
66792Epibacterium mobile strain DSM 23403379347.178wgspatric379347
66792Ruegeria mobilis strain NBRC101030379347.143wgspatric379347
66792Epibacterium mobile NBRC 1010302816332498draftimg379347
66792Epibacterium mobile DSM 234032693429875draftimg379347

GC content

  • @ref: 16845
  • GC-content: 58.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.761yes
anaerobicno99.023yes
halophileyes83.631no
spore-formingno96.31no
glucose-utilyes89.403no
aerobicyes88.262yes
motileyes81.765yes
flagellatedno65.214no
thermophileno95.633no
glucose-fermentno88.976no

External links

@ref: 16845

culture collection no.: DSM 23403, CIP 109181, MBIC 01146, NBRC 101030

straininfo link

  • @ref: 82964
  • straininfo: 199626

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
16845Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23403)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23403
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31871Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128131
37467Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6893
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82964Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID199626.1StrainInfo: A central database for resolving microbial strain identifiers
120987Curators of the CIPCollection of Institut Pasteur (CIP 109181)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109181