Strain identifier

BacDive ID: 137823

Type strain: No

Species: Aggregatibacter actinomycetemcomitans

Strain Designation: B5279

Strain history: CIP <- 1984, ATCC <- 1975, R.E. Weaver, CDC: strain B5279 <- Illinois Dept. Publ. Hlth., USA <- Res. & Ed. Hosp., Chicago, USA

NCBI tax ID(s): 714 (species)

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General

@ref: 38769

BacDive-ID: 137823

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Aggregatibacter actinomycetemcomitans B5279 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Pasteurellaceae.

NCBI tax id

  • NCBI tax id: 714
  • Matching level: species

strain history

@refhistory
67770Y. Kosako <-- R. Sakazaki <-- ATCC 29522 <-- CDC B5279 <-- Illinois Dept. Public Health, USA <-- Res. & Educ. Hosp., Chicago, USA.
38769CIP <- 1984, ATCC <- 1975, R.E. Weaver, CDC: strain B5279 <- Illinois Dept. Publ. Hlth., USA <- Res. & Ed. Hosp., Chicago, USA

doi: 10.13145/bacdive137823.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Aggregatibacter
  • species: Aggregatibacter actinomycetemcomitans
  • full scientific name: Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006

  • synonyms

    @refsynonym
    20215Bacterium actinomycetemcomitans
    20215Haemophilus actinomycetemcomitans
    20215Actinobacillus actinomycetemcomitans

@ref: 38769

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Aggregatibacter

species: Aggregatibacter actinomycetemcomitans

strain designation: B5279

type strain: no

Morphology

cell morphology

  • @ref: 38769
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38769MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
38769CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
38769CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
38769positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 38769
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
38769nitrate+reduction17632
38769nitrite-reduction16301
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377sucrose-builds acid from17992
68377ornithine-degradation18257
68377urea-hydrolysis16199
68377tryptophan-energy source27897

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
3876935581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase+2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase+3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
38769oxidase-
38769catalase+1.11.1.6
38769urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38769-+---+----++--+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
38769------------+/---------+/---------------------------+/-

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
53536-+++----+--+-

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typeisolation date
38769USAUSANorth America
53536Human mandibular abscess
67770Mandibular abscess
38769United States of AmericaUSANorth AmericaHuman, Mandibular abscess1970

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Bone
#Host Body-Site#Oral cavity and airways#Mouth
#Host Body-Site#Other#Wound
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_570.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_321;97_406;98_465;99_570&stattab=map
  • Last taxonomy: Aggregatibacter actinomycetemcomitans subclade
  • 16S sequence: M75036
  • Sequence Identity:
  • Total samples: 8336
  • soil counts: 46
  • aquatic counts: 279
  • animal counts: 7969
  • plant counts: 42

Safety information

risk assessment

  • @ref: 38769
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Aggregatibacter actinomycetemcomitans gene for 16S rRNA, partial sequence, strain: JCM 2434AB5947581462ena714
67770Aggregatibacter actinomycetemcomitans strain ATCC 29522 16S ribosomal RNA gene, partial sequenceM750361479ena714

Genome sequences

  • @ref: 66792
  • description: Aggregatibacter actinomycetemcomitans ATCC 29522
  • accession: GCA_023559645
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 714

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.507no
gram-positiveno98.593no
anaerobicno93.457no
aerobicno96.081no
halophileno52.385no
spore-formingno98.612no
motileno89.075no
glucose-fermentno52.718no
thermophileno95.265yes
glucose-utilno51.169no

External links

@ref: 38769

culture collection no.: CIP 101032, ATCC 29522, NCTC 10979, CCUG 37002, MCCM 02810, JCM 2434

straininfo link

  • @ref: 94640
  • straininfo: 45864

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1398963Identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases.Sorsa T, Ingman T, Suomalainen K, Haapasalo M, Konttinen YT, Lindy O, Saari H, Uitto VJInfect Immun10.1128/iai.60.11.4491-4495.19921992Collagenases/*metabolism, Dental Plaque/enzymology/*microbiology, Endopeptidases/*metabolism, Enzyme Activation, Fibroblasts/enzymology, Glycoproteins/metabolism, Humans, In Vitro Techniques, Matrix Metalloproteinase 1, Matrix Metalloproteinase 8, Neutrophils/enzymology, Periodontitis/enzymology/*microbiology, Tissue Inhibitor of Metalloproteinases, Treponema/enzymologyMetabolism
Pathogenicity1460548The mechanisms of Eikenella corrodens aggregation by salivary glycoprotein and the effect of the glycoprotein on oral bacterial aggregation.Ebisu S, Nakae H, Fukuhara H, Okada HJ Periodontal Res10.1111/j.1600-0765.1992.tb01745.x1992Acetylgalactosamine/pharmacology, Bacterial Adhesion/drug effects/*physiology, Bacterial Outer Membrane Proteins, Eikenella corrodens/chemistry/*physiology, Electrophoresis, Polyacrylamide Gel, Glycoproteins/chemistry/*physiology, Glycosides/pharmacology, Gram-Negative Bacteria/physiology, Gram-Positive Bacteria/physiology, Humans, Lectins/physiology, Neuraminidase/pharmacology, Saliva/microbiology/physiology, Salivary Proteins and Peptides/*physiologyEnzymology
Phylogeny1548238Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences.Dewhirst FE, Paster BJ, Olsen I, Fraser GJJ Bacteriol10.1128/jb.174.6.2002-2013.19921992Base Sequence, DNA, Ribosomal/genetics, Molecular Sequence Data, Oligodeoxyribonucleotides/chemistry, Pasteurellaceae/*genetics, Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence AlignmentGenetics
Biotechnology2134298[Mouse interleukin-1 production by Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide and augmenting effect of indomethacin on its production].Tsutsui T, Hanazawa SMeikai Daigaku Shigaku Zasshi1990Aggregatibacter actinomycetemcomitans/*physiology, Animals, Indomethacin/*pharmacology, Interleukin-1/*biosynthesis, Lipopolysaccharides/*physiology, Macrophages/metabolism, Masoprocol/pharmacology, Mice, Mice, Inbred C3H, Polymyxin B/pharmacologyMetabolism
2489665[Chemical structure and immunomodulating activities of peptidoglycan from Actinobacillus actinomycetemcomitans].Kinoshita FKanagawa Shigaku1989Actinobacillus/*analysis, Bacterial Outer Membrane Proteins/immunology, Diaminopimelic Acid/chemistry/immunology, Hypersensitivity, Delayed/etiology, Ovalbumin/immunology, Peptidoglycan/*chemistry/immunology
Enzymology3655733Chemical composition and immunobiological activities of sodium dodecyl sulphate extracts from the cell envelopes of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Fusobacterium nucleatum.Kato K, Kokeguchi S, Ishihara H, Murayama Y, Tsujimoto M, Takada H, Ogawa T, Kotani SJ Gen Microbiol10.1099/00221287-133-4-10331987Actinobacillus/*analysis, Adjuvants, Immunologic/analysis, Animals, Bacteroides/*analysis, Cell Membrane/analysis, Cell Movement, DNA/biosynthesis, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, Fibroblasts/metabolism, Fusobacterium/*analysis, Guinea Pigs, Humans, Mice, Sodium Dodecyl SulfatePathogenicity
Phylogeny3935663Differentiation among closely related organisms of the Actinobacillus-Haemophilus-Pasteurella group by means of lysozyme and EDTA.Olsen I, Brondz IJ Clin Microbiol10.1128/jcm.22.4.629-636.19851985Actinobacillus/*classification/drug effects/ultrastructure, Bacteriolysis/drug effects, Edetic Acid/*pharmacology, Haemophilus/*classification/drug effects/ultrastructure, Hydrogen-Ion Concentration, Microscopy, Electron, Muramidase/*pharmacology, Pasteurella/*classification/drug effects/ultrastructure, Species Specificity, Temperature, Tromethamine/pharmacologyPathogenicity
Phylogeny6735473Immunoglobulin G response to subgingival gram-negative bacteria in human subjects.Naito Y, Okuda K, Takazoe IInfect Immun10.1128/iai.45.1.47-51.19841984Adult, *Antibody Formation, Bacteroides/immunology, Enzyme-Linked Immunosorbent Assay, Fusobacterium/immunology, Gingiva/*microbiology, Gram-Negative Bacteria/*immunology/isolation & purification, Humans, Immunoglobulin G/*analysis, Periodontitis/*microbiology, Reference Values, Species SpecificityPathogenicity
Metabolism7054125Characterization of an inducible bacteriophage from a leukotoxic strain of Actinobacillus actinomycetemcomitans.Stevens RH, Hammond BF, Lai CHInfect Immun10.1128/iai.35.1.343-349.19821982*Actinobacillus/metabolism, Bacteriophages/growth & development/*isolation & purification/ultrastructure, Centrifugation, Density Gradient, DNA, Viral/analysis, Exotoxins/*biosynthesis, Virus ActivationEnzymology
Pathogenicity8406888Association of Actinobacillus actinomycetemcomitans leukotoxin with nucleic acids on the bacterial cell surface.Ohta H, Hara H, Fukui K, Kurihara H, Murayama Y, Kato KInfect Immun10.1128/iai.61.11.4878-4884.19931993Aggregatibacter actinomycetemcomitans/*chemistry/pathogenicity, Bacterial Toxins/*analysis, DNA, Bacterial/*analysis, Exotoxins/*analysis/toxicity, Humans, Neutrophils/drug effects, Osmolar Concentration, Sodium Chloride/pharmacology
Pathogenicity9573812In vitro antimicrobial susceptibility of periodontopathic Actinobacillus actinomycetemcomitans to roxithromycin and erythromycin.Piccolomini R, Di Bonaventura G, Catamo G, Picciani C, Paolantonio MOral Microbiol Immunol10.1111/j.1399-302x.1997.tb00740.x1997Adolescent, Adult, Aggregatibacter actinomycetemcomitans/*drug effects/isolation & purification, Aggressive Periodontitis/*microbiology, Anti-Bacterial Agents/*pharmacology, Child, Dose-Response Relationship, Drug, Erythromycin/*pharmacology, Female, Humans, Male, Microbial Sensitivity Tests/methods/statistics & numerical data, Periodontitis/*microbiology, Roxithromycin/*pharmacologyEnzymology
Pathogenicity10530260An evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.D'Arcangelo C, Varvara G, De Fazio PJ Endod10.1016/S0099-2399(06)81170-21999Anti-Infective Agents, Local/administration & dosage/*pharmacology, Bacteria, Anaerobic/*drug effects, Cetrimonium, Cetrimonium Compounds/administration & dosage/pharmacology, Chlorhexidine/administration & dosage/pharmacology, Colony Count, Microbial, Dose-Response Relationship, Drug, Edetic Acid/administration & dosage/pharmacology, Root Canal Irrigants/administration & dosage/*pharmacology, Sodium Hypochlorite/administration & dosage/pharmacology
Pathogenicity10948136A novel factor isolated from Actinobacillus actinomycetemcomitans stimulates mouse B cells and human peripheral blood mononuclear cells.Jeong SJ, Yee ST, Jo WS, Yu SH, Lee SH, Lim YJ, Yoo YH, Kim JM, Lee JD, Jeong MHInfect Immun10.1128/IAI.68.9.5132-5138.20002000Adjuvants, Immunologic/*isolation & purification/pharmacology, Aggregatibacter actinomycetemcomitans/*chemistry, Animals, B-Lymphocytes/*drug effects/immunology, Electrophoresis, Polyacrylamide Gel, Female, Humans, Lipopolysaccharides/pharmacology, Lymphocyte Activation/*drug effects, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Weight, Receptors, Antigen, T-Cell, alpha-beta/physiologyEnzymology
Enzymology11548202Detection of antigenic surface proteins of Actinobacillus actinomycetemcomitans using the immunoblotting method.Mineyama RMicrobios2001Actinobacillus Infections/microbiology, Aggregatibacter actinomycetemcomitans/*isolation & purification, Antibodies, Bacterial/blood/*immunology, Antigens, Bacterial/*analysis, Antigens, Surface/*analysis, *Blotting, Western/*methods, Electrophoresis, Polyacrylamide Gel, Humans, Immunoglobulin G/blood/immunology, Immunoglobulin M/blood/immunologyPathogenicity
Enzymology11982335Parameters associated with cloning in Actinobacillus actinomycetemcomitans.Galli DM, Kerr MS, Fair AD, Permpanich P, LeBlanc DJPlasmid10.1006/plas.2001.15562002Aggregatibacter actinomycetemcomitans/*genetics, Chromosomes, Bacterial, Cloning, Molecular/*methods, DNA Restriction Enzymes/genetics/metabolism, DNA Restriction-Modification Enzymes/genetics/metabolism, DNA Transposable Elements, Electroporation, Escherichia coli/genetics, Plasmids, Recombinant Proteins/*genetics/metabolism, Replicon, *Transformation, BacterialMetabolism
Pathogenicity17356375Antibacterial effect of zinc phosphate mineralized guided bone regeneration membranes.Chou AH, LeGeros RZ, Chen Z, Li YImplant Dent10.1097/ID.0b013e318031224a2007Aggregatibacter actinomycetemcomitans/*drug effects, Animals, Anti-Infective Agents, Local/analysis/*pharmacology, Bone Regeneration, Cattle, Collagen, Colony Count, Microbial, Guided Tissue Regeneration, Periodontal/*methods, *Membranes, Artificial, Phosphates/analysis/*pharmacology, Polyglactin 910, Zinc Compounds/analysis/*pharmacology
Pathogenicity19572889Protease-activated receptor 2 mediates interleukin-8 and intercellular adhesion molecule-1 expression in response to Aggregatibacter actinomycetemcomitans.Shimada T, Sugano N, Ikeda K, Shimada K, Iizuka T, Ito KOral Microbiol Immunol10.1111/j.1399-302X.2009.00507.x2009Aggregatibacter actinomycetemcomitans/drug effects/*enzymology, Cell Line, Tumor, Epithelial Cells/*metabolism, Gingiva/cytology/metabolism/*microbiology, Humans, Intercellular Adhesion Molecule-1/*biosynthesis, Interleukin-8/*biosynthesis, RNA Interference, Receptor, PAR-2/genetics/*physiology, Reverse Transcriptase Polymerase Chain Reaction, Serine Endopeptidases/metabolism, Serine Proteinase Inhibitors/pharmacology, Transfection, Up-RegulationMetabolism
23049613Antibacterial Activity of Myristica fragrans against Oral Pathogens.Shafiei Z, Shuhairi NN, Md Fazly Shah Yap N, Harry Sibungkil CA, Latip JEvid Based Complement Alternat Med10.1155/2012/8253622012

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38769Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101032Collection of Institut Pasteur (CIP 101032)
53536Curators of the CCUGhttps://www.ccug.se/strain?id=37002Culture Collection University of Gothenburg (CCUG) (CCUG 37002)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
94640Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID45864.1