Strain identifier

BacDive ID: 1378

Type strain: Yes

Species: Oceanobacillus kapialis

Strain Designation: SSK2-2

Strain history: <- S Tanasupawat, Chulalongkorn Univ., Thailand

NCBI tax ID(s): 481353 (species)

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General

@ref: 16738

BacDive-ID: 1378

DSM-Number: 23158

keywords: 16S sequence, Bacteria, obligate aerobe, moderately halophilic, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Oceanobacillus kapialis SSK2-2 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from fermented shrimp paste .

NCBI tax id

  • NCBI tax id: 481353
  • Matching level: species

strain history

@refhistory
16738<- KCTC <- S. Tanasupawat, Dept. Microbiology, Chulalongkorn Univ., Thailand
67771<- S Tanasupawat, Chulalongkorn Univ., Thailand

doi: 10.13145/bacdive1378.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oceanobacillus
  • species: Oceanobacillus kapialis
  • full scientific name: Oceanobacillus kapialis Namwong et al. 2009

@ref: 16738

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Oceanobacillus

species: Oceanobacillus kapialis

full scientific name: Oceanobacillus kapialis Namwong et al. 2009

strain designation: SSK2-2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23012positive0.8-3.0 µm0.4-0.5 µmrod-shapedyesperitrichous
67771rod-shapedyesperitrichous
67771positive

colony morphology

  • @ref: 23012
  • colony size: 0.3-2.7 mm
  • colony color: white to cream
  • colony shape: circular

Culture and growth conditions

culture medium

  • @ref: 16738
  • name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/372
  • composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372; with strain-specific modifications) Composition: NaCl 100.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16738positivegrowth30mesophilic
23012positivegrowth8.0-43.0
23012positiveoptimum37.0mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23012positivegrowth6.0-9.0alkaliphile
23012positiveoptimum8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23012obligate aerobe
67771aerobe

spore formation

@refspore descriptiontype of sporespore formation
23012oval,terminal,in swollen sporangiaendosporeyes
67771endosporeyes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23012moderately halophilic
23012NaClpositivegrowth0.5-24.0 %(w/v)
23012NaCloptimum6.0-14.0 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2301227613amygdalin-builds acid from
2301217057cellobiose-builds acid from
2301212936D-galactose-builds acid from
230128391D-gluconate-builds acid from
2301216988D-ribose-builds acid from
2301217924D-sorbitol-builds acid from
2301265327D-xylose-builds acid from
230124853esculin-builds acid from
2301217754glycerol-builds acid from
2301215443inulin-builds acid from
2301262345L-rhamnose-builds acid from
2301217716lactose-builds acid from
230126731melezitose-builds acid from
2301228053melibiose-builds acid from
23012320061methyl alpha-D-glucopyranoside-builds acid from
2301217268myo-inositol-builds acid from
2301216634raffinose-builds acid from
2301217814salicin-builds acid from
2301217992sucrose-builds acid from
2301227082trehalose-builds acid from
230124853esculin-hydrolysis
2301217368hypoxanthine-hydrolysis
2301228017starch-hydrolysis
2301253426tween 80-hydrolysis
2301218186tyrosine-hydrolysis
2301215318xanthine-hydrolysis
2301217632nitrate-reduction
2301215824D-fructose+builds acid from
2301217634D-glucose+builds acid from
2301216899D-mannitol+builds acid from
2301216024D-mannose+builds acid from
2301230849L-arabinose+builds acid from
2301217306maltose+builds acid from
23012casein+hydrolysis
230125291gelatin+hydrolysis

enzymes

@refvalueactivityec
23012catalase+1.11.1.6
23012cytochrome oxidase+1.9.3.1
23012DNase-
23012urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
23012fermented shrimp paste (ka-pi)market in Samutsakorn ProvinceThailandTHAAsiaagar plates of Lentibacillus medium (JCM medium no. 377),pH 7.2containing (l-1): 100 g NaCl,5 g Casamino acids, 5 g yeast extract, 1 g glutamic acid, 2 g KCl, 3 g trisodium citrate, 20 g MgSO4.7H2O, 36 mg FeCl2.4H2O, 0.36 mg MnCl2.4H2O and 20 g agar7 days37.0
16738fermented shrimp paste (ka-pi)Samutsakorn ProvinceThailandTHAAsia
67771From Shrimp paste (ka-pi)ThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Seafood
#Engineered#Food production#Fermented

Safety information

risk assessment

  • @ref: 16738
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16738
  • description: Oceanobacillus kapialis gene for 16S ribosomal RNA, partial sequence, strain: SSK2-2
  • accession: AB366005
  • length: 1487
  • database: ena
  • NCBI tax ID: 481353

GC content

@refGC-contentmethod
1673839.7high performance liquid chromatography (HPLC)
6777139.7

External links

@ref: 16738

culture collection no.: DSM 23158, KCTC 13177, PCU 300, TISTR 1858

straininfo link

  • @ref: 71029
  • straininfo: 399584

literature

  • topic: Phylogeny
  • Pubmed-ID: 19620352
  • title: Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand.
  • authors: Namwong S, Tanasupawat S, Lee KC, Lee JS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007161-0
  • year: 2009
  • mesh: Bacillaceae/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, *Food Microbiology, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Thailand, Vitamin K 2/analysis
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16738Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23158)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23158
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23012Sirilak Namwong,Somboon Tanasupawat,Keun Chul Lee,Jung-Sook Lee10.1099/ijs.0.007161-0Oceanobacillus kapialis sp. nov., from fermented shrimp paste in ThailandIJSEM 59: 2254-2259 200919620352
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
71029Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399584.1StrainInfo: A central database for resolving microbial strain identifiers