Strain identifier

BacDive ID: 137718

Type strain: Yes

Species: Azospirillum lipoferum

Strain history: CIP <- 1999, NCAIM <- DSMZ <- ATCC <- N.R. Krieg <- J. Döbereiner

NCBI tax ID(s): 193 (species)

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General

@ref: 38613

BacDive-ID: 137718

DSM-Number: 1691

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Azospirillum lipoferum CCUG 56042 is a mesophilic, Gram-negative, motile bacterium of the family Azospirillaceae.

NCBI tax id

  • NCBI tax id: 193
  • Matching level: species

strain history

@refhistory
67770N. R. Krieg Sp 59b <-- J. Döbereiner.
38613CIP <- 1999, NCAIM <- DSMZ <- ATCC <- N.R. Krieg <- J. Döbereiner

doi: 10.13145/bacdive137718.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Azospirillaceae
  • genus: Azospirillum
  • species: Azospirillum lipoferum
  • full scientific name: Azospirillum lipoferum (Beijerinck 1925) Tarrand et al. 1979 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Spirillum lipoferum

@ref: 38613

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodospirillales

family: Rhodospirillaceae

genus: Azospirillum

species: Azospirillum lipoferum

type strain: yes

Morphology

cell morphology

  • @ref: 38613
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38613MEDIUM 321 - for Azospirillum lipoferumyesDistilled water make up to (950.000 ml);Sodium chloride (0.100 g);Iron (II) sulphate heptahydrate (0.010 g);Manganese II sulphate monohydrate (2.000 mg);Magnesium sulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.020 g);Sodium molybdate dihydr
38613CIP Medium 321yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=321

culture temp

@refgrowthtypetemperaturerange
38613positivegrowth30mesophilic
67770positivegrowth30mesophilic
38613positivegrowth25-41
38613nogrowth5psychrophilic
38613nogrowth15psychrophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
38613NaClpositivegrowth0-2 %
38613NaClnogrowth4 %
38613NaClnogrowth6 %
38613NaClnogrowth8 %
38613NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose+builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68368nitrate-reduction17632
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-glucose-fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
38613D-arabinose-degradation17108
38613D-fructose-degradation15824
38613D-glucose-degradation17634
38613D-mannose-degradation16024
38613D-xylose-degradation65327
38613cellobiose-degradation17057
38613lactose-degradation17716
38613maltose-degradation17306
38613salicin-degradation17814
38613sucrose-degradation17992
38613citrate+carbon source16947
38613esculin+hydrolysis4853
38613glucose+fermentation17234
38613lactose-fermentation17716
38613nitrate+reduction17632
38613nitrite+reduction16301
38613sodium thiosulfate-builds gas from132112
38613glucose+degradation17234
38613nitrate+respiration17632

antibiotic resistance

  • @ref: 38613
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
3861335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
3861315688acetoin+
3861317234glucose+

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
38613oxidase+
38613beta-galactosidase+3.2.1.23
38613alcohol dehydrogenase-1.1.1.1
38613gelatinase-
38613amylase-
38613DNase-
38613caseinase-3.4.21.50
38613catalase-1.11.1.6
38613tween esterase-
38613gamma-glutamyltransferase+2.3.2.2
38613lecithinase-
38613lipase-
38613lysine decarboxylase-4.1.1.18
38613ornithine decarboxylase-4.1.1.17
38613phenylalanine ammonia-lyase+4.3.1.24
38613protease-
38613tryptophan deaminase-
38613urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38613-+-+-+++--++-+--+---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUINOSorRHASACMELNO2N2
38613-------------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
38613---+/-+/-----+/-+/-+/----------+--++----------------+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
38613+++-+-------------++++--+------+++--+------++-+-++++-++++--+++++++-----+-+-++-+++--++---++++-++-+++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
38613Rio de JaneiroBrazilBRAMiddle and South America
60842Roots,wheat
67770Rio de JaneiroBrazilBRAMiddle and South AmericaWheat
38613Rio de JaneiroBrazilBRAMiddle and South AmericaFood, Wheat

Safety information

risk assessment

  • @ref: 38613
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Azospirillum lipoferum gene for 16S rRNA, partial sequence, strain: NBRC 102290AB6817461417ena193
67770Azospirillum lipoferum strain DSM 1691 16S ribosomal RNA gene, partial sequenceGU2564411478ena193
67770Azospirillum lipoferum 16S ribosomal RNAM590611439ena193
67770A.lipoferum (ncimb 11861) 16S ribosomal RNAZ296191432ena193

GC content

  • @ref: 67770
  • GC-content: 70
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 38613

culture collection no.: CCUG 56042, NCIMB 11861, LMG 13128, CCM 3863, JCM 1247, CIP 106280, ATCC 29707, DSM 1691, NCAIM B.01801, BCRC 12213, IAM 12399, ICMP 15216, NBRC 102290, NRRL B-14654, VKM B-1519

straininfo link

  • @ref: 94549
  • straininfo: 7463

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny356945A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.Tarrand JJ, Krieg NR, Dobereiner JCan J Microbiol10.1139/m78-1601978Culture Media, Culture Techniques, DNA, Bacterial/*analysis, Microscopy, Electron, Microscopy, Phase-Contrast, Nucleic Acid Denaturation, Spirillum/*classification/cytology/physiology, Terminology as TopicCultivation
Phylogeny3894333Flocculation in Azospirillum brasilense and Azospirillum lipoferum: exopolysaccharides and cyst formation.Sadasivan L, Neyra CAJ Bacteriol10.1128/jb.163.2.716-723.19851985Aerobiosis, Cell Survival, Flocculation, Gram-Negative Aerobic Bacteria/cytology/growth & development/*physiology, Microscopy, Electron, Microscopy, Phase-Contrast, Polysaccharides, Bacterial/*analysis, Species Specificity, Spores, Bacterial/physiology
Metabolism6588050Catabolism of carbohydrates and organic acids and expression of nitrogenase by azospirilla.Martinez-Drets G, Del Gallo M, Burpee C, Burris RHJ Bacteriol10.1128/jb.159.1.80-85.19841984*Carbohydrate Metabolism, Carbon Radioisotopes, Carboxylic Acids/*metabolism, Citric Acid Cycle, Fructose/metabolism, Glucose/metabolism, Gram-Negative Aerobic Bacteria/genetics/*metabolism, Hexosephosphates/metabolism, Ketoglutaric Acids/metabolism, Kinetics, Nitrogenase/*genetics, Species Specificity, Sucrose/metabolismEnzymology
32544804Enrichment of hydrogen-oxidizing bacteria with nitrate recovery as biofertilizers in the mixed culture.Zhang W, Niu Y, Li YX, Zhang F, Jianxiong Zeng RBioresour Technol10.1016/j.biortech.2020.1236452020*Bacteria, Bioreactors, Denitrification, *Hydrogen, Nitrates, Nitrogen, Oxidation-Reduction
Metabolism33220977Two-stage enrichment of hydrogen-oxidizing bacteria as biofertilizers.Zhang W, Li YX, Niu Y, Zhang F, Li YB, Zeng RJChemosphere10.1016/j.chemosphere.2020.1289322020Azoarcus/metabolism, *Bacteria/metabolism, Carbon-Carbon Lyases/metabolism, *Hydrogen, Oxidation-Reduction

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38613Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106280Collection of Institut Pasteur (CIP 106280)
60842Curators of the CCUGhttps://www.ccug.se/strain?id=56042Culture Collection University of Gothenburg (CCUG) (CCUG 56042)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
94549Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID7463.1