Strain identifier

BacDive ID: 137694

Type strain: Yes

Species: Brachyspira alvinipulli

Strain history: CIP <- 1998, T. Stanton, Nat. Animal Dis. Center, USDA-ARS, USA: strain C-1, Serpulina alvinipulli

NCBI tax ID(s): 1408430 (strain), 84379 (species)

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General

@ref: 38570

BacDive-ID: 137694

keywords: genome sequence, Bacteria, anaerobe, mesophilic, Gram-negative

description: Brachyspira alvinipulli CIP 105681 is an anaerobe, mesophilic, Gram-negative bacterium of the family Brachyspiraceae.

NCBI tax id

NCBI tax idMatching level
84379species
1408430strain

strain history

  • @ref: 38570
  • history: CIP <- 1998, T. Stanton, Nat. Animal Dis. Center, USDA-ARS, USA: strain C-1, Serpulina alvinipulli

doi: 10.13145/bacdive137694.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Brachyspirales
  • family: Brachyspiraceae
  • genus: Brachyspira
  • species: Brachyspira alvinipulli
  • full scientific name: Brachyspira alvinipulli Stanton et al. 1998

  • synonyms

    • @ref: 20215
    • synonym: Serpulina alvinipulli

@ref: 38570

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Brachyspirales

family: Brachyspiraceae

genus: Brachyspira

species: Brachyspira alvinipulli

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.806
38570negativerod-shapedyes

colony morphology

  • @ref: 38570

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38570MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
38570CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

  • @ref: 38570
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 69480
  • oxygen tolerance: anaerobe
  • confidence: 99.589

spore formation

@refspore formationconfidence
69481no94
69480no99.976

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
38570esculin+hydrolysis4853
38570hippurate+hydrolysis606565
38570nitrate-reduction17632
38570nitrite-reduction16301

metabolite production

  • @ref: 38570
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
38570oxidase-
38570beta-galactosidase+3.2.1.23
38570gelatinase-
38570amylase-
38570DNase-
38570caseinase-3.4.21.50
38570catalase+1.11.1.6
38570lecithinase-
38570lipase-
38570protease-
38570urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38570-+++------++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
38570-------------------------------------------------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
38570OhioUSAUSANorth America
38570OhioUnited States of AmericaUSANorth AmericaChicken, cecum

Safety information

risk assessment

  • @ref: 38570
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brachyspira alvinipulli ATCC 51933GCA_000518245scaffoldncbi1408430
66792Brachyspira alvinipulli ATCC 519331408430.3wgspatric1408430
66792Brachyspira alvinipulli ATCC 519332545555860draftimg1408430

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
motileyes66.48no
flagellatedno86.933no
gram-positiveno96.061no
anaerobicyes96.04no
halophileno76.561no
spore-formingno94.049no
thermophileno79.204yes
glucose-utilyes66.925no
aerobicno98.221no
glucose-fermentno50.513no

External links

@ref: 38570

culture collection no.: CIP 105681, ATCC 51933

straininfo link

  • @ref: 94529
  • straininfo: 43249

literature

topicPubmed-IDtitleauthorsjournalDOIyearmesh
Phylogeny9734021Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens.Stanton TB, Postic D, Jensen NSInt J Syst Bacteriol10.1099/00207713-48-3-6691998Animals, Base Composition, Brachyspira/*classification/genetics/physiology, Chickens/*microbiology, DNA, Bacterial/analysis, Intestinal Diseases/etiology/*veterinary, Polymorphism, Restriction Fragment Length
Enzymology15297747A possibility of application of the 105-kilodaltons protein of Brachyspira alvinipulli cross-reacting with antisera to five species in the genus Brachyspira to diagnosis.Abe Y, Adachi YJ Vet Med Sci10.1292/jvms.66.7732004Animals, Antibodies, Bacterial/*immunology, Antigens, Bacterial/*immunology, Bacterial Proteins/*immunology, Chickens, Cross Reactions/immunology, Electrophoresis, Polyacrylamide Gel/veterinary, Immunodiffusion/veterinary, Molecular Weight, Poultry Diseases/*diagnosis, Spirochaeta/*immunology, Spirochaetales Infections/diagnosis/*veterinary, Swine

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38570Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105681Collection of Institut Pasteur (CIP 105681)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
94529Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID43249.1