Strain identifier
BacDive ID: 13769
Type strain: 
Species: Aliiroseovarius crassostreae
Strain Designation: CV919-312, CVSP
Strain history: <- K. J. Boettcher
NCBI tax ID(s): 154981 (species)
version 8.1 (current version)
General
@ref: 6663
BacDive-ID: 13769
DSM-Number: 16950
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, animal pathogen
description: Aliiroseovarius crassostreae CV919-312 is an aerobe, mesophilic, Gram-negative animal pathogen that was isolated from Eastern oyster affected by juvenile oyster disease.
NCBI tax id
- NCBI tax id: 154981
- Matching level: species
strain history
- @ref: 6663
- history: <- K. J. Boettcher
doi: 10.13145/bacdive13769.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodobacterales
- family: Roseobacteraceae
- genus: Aliiroseovarius
- species: Aliiroseovarius crassostreae
- full scientific name: Aliiroseovarius crassostreae (Boettcher et al. 2005) Park et al. 2015
synonyms
@ref synonym 20215 Roseovarius crassostreae 20215 Pseudoroseovarius crassostreae
@ref: 6663
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhodobacterales
family: Rhodobacteraceae
genus: Aliiroseovarius
species: Aliiroseovarius crassostreae
full scientific name: Aliiroseovarius crassostreae (Boettcher et al. 2005) Park et al. 2015
strain designation: CV919-312, CVSP
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 31463 | negative | 2.8 µm | 0.9 µm | rod-shaped | yes | |
| 69480 | negative | 99.997 |
colony morphology
- @ref: 6663
- incubation period: 1-2 days
pigmentation
- @ref: 31463
- production: no
Culture and growth conditions
culture medium
- @ref: 6663
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6663 | positive | growth | 28 | mesophilic |
| 31463 | positive | optimum | 34 | mesophilic |
culture pH
- @ref: 31463
- ability: positive
- type: optimum
- pH: 7.25
Physiology and metabolism
oxygen tolerance
- @ref: 31463
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.992 |
halophily
- @ref: 31463
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 1.25 %
observation
- @ref: 31463
- observation: aggregates in clumps
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31463 | 17632 | nitrate | + | reduction |
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | - | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | - | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 6663 | catalase | + | 1.11.1.6 |
| 6663 | cytochrome-c oxidase | + | 1.9.3.1 |
| 31463 | acid phosphatase | + | 3.1.3.2 |
| 31463 | alkaline phosphatase | + | 3.1.3.1 |
| 31463 | catalase | + | 1.11.1.6 |
| 31463 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 6663 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 6663
- sample type: Eastern oyster (Crassostrea virginica) affected by juvenile oyster disease
- host species: Crassostrea virginica
- geographic location: Maine, Damariscotta River
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
- Cat1: #Host
- Cat2: #Invertebrates (Other)
- Cat3: #Mollusca
taxonmaps
- @ref: 69479
- File name: preview.99_56.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_46;97_48;98_52;99_56&stattab=map
- Last taxonomy: Aliiroseovarius crassostreae subclade
- 16S sequence: AF114484
- Sequence Identity:
- Total samples: 446
- soil counts: 5
- aquatic counts: 405
- animal counts: 33
- plant counts: 3
Safety information
risk assessment
- @ref: 6663
- pathogenicity animal: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6663
- description: Roseovarius crassostreae isolate CV919-312 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence
- accession: AF114484
- length: 2542
- database: ena
- NCBI tax ID: 154981
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Aliiroseovarius crassostreae CV919-312 | GCA_001307765 | contig | ncbi | 154981 |
| 66792 | Aliiroseovarius crassostreae DSM 16950 | GCA_900116725 | scaffold | ncbi | 154981 |
| 66792 | Aliiroseovarius crassostreae strain DSM 16950 | 154981.7 | wgs | patric | 154981 |
| 66792 | Aliiroseovarius crassostreae CV919-312 | 2645727822 | draft | img | 154981 |
| 66792 | Aliiroseovarius crassostreae DSM 16950 | 2619619030 | draft | img | 154981 |
GC content
- @ref: 31463
- GC-content: 59
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | yes | 69.534 | yes |
| flagellated | no | 69.225 | no |
| gram-positive | no | 98.535 | yes |
| anaerobic | no | 97.007 | no |
| aerobic | yes | 72.806 | no |
| halophile | yes | 61.334 | yes |
| spore-forming | no | 95.725 | no |
| glucose-ferment | no | 91.739 | yes |
| thermophile | no | 96.098 | no |
| glucose-util | no | 66.412 | yes |
External links
@ref: 6663
culture collection no.: DSM 16950, ATCC BAA 1102
straininfo link
- @ref: 82948
- straininfo: 265407
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16014477 | Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. | Boettcher KJ, Geaghan KK, Maloy AP, Barber BJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.63620-0 | 2005 | Animals, *Aquaculture, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Maine, Massachusetts, Molecular Sequence Data, New York, Ostreidae/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Rhodobacteraceae/*classification/genetics/metabolism/*pathogenicity, Roseobacter/classification, Sequence Analysis, DNA, Species Specificity | Genetics |
| Phylogeny | 20348324 | Roseovarius marinus sp. nov., isolated from seawater. | Jung YT, Lee JS, Oh KH, Oh TK, Yoon JH | Int J Syst Evol Microbiol | 10.1099/ijs.0.019828-0 | 2010 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Likelihood Functions, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/analysis, *Water Microbiology | Genetics |
| Phylogeny | 21669917 | Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi. | Kim YO, Kong HJ, Park S, Kang SJ, Kim WJ, Kim KK, Oh TK, Yoon JH | Int J Syst Evol Microbiol | 10.1099/ijs.0.031674-0 | 2011 | Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Sequence Analysis, DNA, Ubiquinone/chemistry, Urochordata/*microbiology | Genetics |
| Phylogeny | 22941298 | Roseovarius sediminilitoris sp. nov., isolated from seashore sediment. | Park S, Yoon JH | Int J Syst Evol Microbiol | 10.1099/ijs.0.043737-0 | 2012 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analysis | Genetics |
| Phylogeny | 25964517 | Aliiroseovarius pelagivivens gen. nov., sp. nov., isolated from seawater, and reclassification of three species of the genus Roseovarius as Aliiroseovarius crassostreae comb. nov., Aliiroseovarius halocynthiae comb. nov. and Aliiroseovarius sediminilitoris comb. nov. | Park S, Park JM, Kang CH, Yoon JH | Int J Syst Evol Microbiol | 10.1099/ijs.0.000315 | 2015 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry | Genetics |
| Phylogeny | 26066711 | Pseudoroseovarius zhejiangensis gen. nov., sp. nov., a novel alpha-proteobacterium isolated from the chemical wastewater, and reclassification of Roseovarius crassostreae as Pseudoroseovarius crassostreae comb. nov., Roseovarius sediminilitoris as Pseudoroseovarius sediminilitoris comb. nov. and Roseovarius halocynthiae as Pseudoroseovarius halocynthiae comb. nov. | Sun C, Pan J, Zhang XQ, Su Y, Wu M | Antonie Van Leeuwenhoek | 10.1007/s10482-015-0480-9 | 2015 | Aerobiosis, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/*isolation & purification/physiology, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Waste Water/*microbiology | Genetics |
| Genetics | 26988054 | Draft Genome Sequence of Aliiroseovarius crassostreae CV919-312, the Causative Agent of Roseovarius Oyster Disease (Formerly Juvenile Oyster Disease). | Kessner L, Spinard E, Gomez-Chiarri M, Rowley DC, Nelson DR | Genome Announc | 10.1128/genomeA.00148-16 | 2016 | ||
| Metabolism | 30297608 | Cyclopropane-Containing Fatty Acids from the Marine Bacterium Labrenzia sp. 011 with Antimicrobial and GPR84 Activity. | Amiri Moghaddam J, Davila-Cespedes A, Kehraus S, Crusemann M, Kose M, Muller CE, Konig GM | Mar Drugs | 10.3390/md16100369 | 2018 | Animals, Anti-Bacterial Agents/metabolism/*pharmacology, Aquatic Organisms/*metabolism, Cyclopropanes/metabolism/*pharmacology, Fatty Acids/metabolism/*pharmacology, Methyltransferases/metabolism, Ostreidae/microbiology, Receptors, Cell Surface/*metabolism, Rhodobacteraceae/*metabolism, beta-Arrestins/metabolism | |
| Phylogeny | 31622234 | Aliiroseovarius marinus sp. nov., isolated from seawater. | Wang Y, Zhang Y, Guo C, Liu T, Dai Y, Lin M, Cui C, Zhang Y, Zhou C, Zhu W | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003757 | 2020 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry, *Water Microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 6663 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16950) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16950 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 31463 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27764 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 68369 | Automatically annotated from API 20NE | ||||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
| 82948 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID265407.1 | StrainInfo: A central database for resolving microbial strain identifiers |