Strain identifier

BacDive ID: 137424

Type strain: No

Species: Nocardia pseudobrasiliensis

Strain Designation: N 649

Strain history: CIP <- 1995, R. Ruimy, CNRS, Villefranche/Mer, France: strain N 649 <- R.J. Wallace, Texas, USA

NCBI tax ID(s): 45979 (species)

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General

@ref: 37938

BacDive-ID: 137424

keywords: Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Nocardia pseudobrasiliensis N 649 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Nocardiaceae.

NCBI tax id

  • NCBI tax id: 45979
  • Matching level: species

strain history

  • @ref: 37938
  • history: CIP <- 1995, R. Ruimy, CNRS, Villefranche/Mer, France: strain N 649 <- R.J. Wallace, Texas, USA

doi: 10.13145/bacdive137424.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia pseudobrasiliensis
  • full scientific name: Nocardia pseudobrasiliensis Ruimy et al. 1996

@ref: 37938

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Mycobacteriales

family: Nocardiaceae

genus: Nocardia

species: Nocardia pseudobrasiliensis

strain designation: N 649

type strain: no

Morphology

cell morphology

  • @ref: 37938
  • gram stain: positive
  • cell shape: rod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37938MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
37938CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
37938positivegrowth30mesophilic
37938positivegrowth30-37mesophilic
37938nogrowth10psychrophilic
37938nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 37938
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
37938NaClpositivegrowth0-4 %
37938NaClnogrowth6 %
37938NaClnogrowth8 %
37938NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
379384853esculin+hydrolysis
37938606565hippurate-hydrolysis
3793817632nitrate+reduction
3793816301nitrite-reduction
3793817632nitrate-respiration

metabolite production

  • @ref: 37938
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 37938
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
37938oxidase-
37938beta-galactosidase-3.2.1.23
37938alcohol dehydrogenase-1.1.1.1
37938gelatinase+
37938amylase-
37938DNase+
37938caseinase+3.4.21.50
37938catalase+1.11.1.6
37938tween esterase-
37938lecithinase-
37938lysine decarboxylase-4.1.1.18
37938ornithine decarboxylase-4.1.1.17
37938urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
37938-+++-++---++---+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
37938++++--------------++-----------++--------------+-+----++---+------------++-----++-------++++-++--++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
37938TexasUSAUSANorth America
37938TexasUnited States of AmericaUSANorth AmericaHuman, Sputum

Safety information

risk assessment

  • @ref: 37938
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 37938

culture collection no.: CIP 104602

straininfo link

  • @ref: 94321
  • straininfo: 69526

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
37938Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104602Collection of Institut Pasteur (CIP 104602)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
94321Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69526.1