Strain identifier
BacDive ID: 1373
Type strain:
Species: Oceanobacillus picturae
Strain Designation: mccs2171
Strain history: CIP <- 2004, DSMZ <- LMG <- J. Heyrman, Univ. Ghent, Belgium: strain mccs2171
NCBI tax ID(s): 171693 (species)
General
@ref: 5586
BacDive-ID: 1373
DSM-Number: 14867
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Oceanobacillus picturae mccs2171 is an aerobe, spore-forming, mesophilic bacterium that was isolated from mural painting.
NCBI tax id
- NCBI tax id: 171693
- Matching level: species
strain history
@ref | history |
---|---|
5586 | <- D. Janssens, LMG; mccs2171 <- J. Heyrman, Univ. Ghent, Belgium |
67771 | <- LMG, mccs2171 <- J. Heyrman, Univ. Ghent Belgium |
121823 | CIP <- 2004, DSMZ <- LMG <- J. Heyrman, Univ. Ghent, Belgium: strain mccs2171 |
doi: 10.13145/bacdive1373.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Oceanobacillus
- species: Oceanobacillus picturae
- full scientific name: Oceanobacillus picturae (Heyrman et al. 2003) Lee et al. 2006
synonyms
- @ref: 20215
- synonym: Virgibacillus picturae
@ref: 5586
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Oceanobacillus
species: Oceanobacillus picturae
full scientific name: Oceanobacillus picturae (Heyrman et al. 2003) Lee et al. 2006
strain designation: mccs2171
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility | flagellum arrangement |
---|---|---|---|---|
23030 | positive | rod-shaped | yes | peritrichous |
67771 | rod-shaped | yes | ||
67771 | positive | |||
121823 | positive | rod-shaped | yes |
colony morphology
- @ref: 121823
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5586 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
33218 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
121823 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
121823 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5586 | positive | growth | 28 | mesophilic |
23030 | positive | growth | 5.0-42.0 | |
33218 | positive | growth | 30 | mesophilic |
60324 | positive | growth | 30-37 | mesophilic |
67771 | positive | growth | 28 | mesophilic |
121823 | positive | growth | 22-37 | |
121823 | no | growth | 10 | psychrophilic |
121823 | no | growth | 45 | thermophilic |
121823 | no | growth | 55 | thermophilic |
culture pH
- @ref: 121823
- ability: positive
- type: growth
- pH: 6
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
60324 | aerobe |
67771 | aerobe |
121823 | obligate aerobe |
spore formation
@ref | spore description | type of spore | spore formation |
---|---|---|---|
23030 | ellipsoidal,subterminal or terminal,in swollen sporangia | spore | yes |
121823 | yes |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
23030 | NaCl | positive | growth | 0.0-22.0 %(w/v) |
121823 | NaCl | positive | growth | 0-10 % |
observation
- @ref: 67771
- observation: quinones: MK-7
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121823 | 16947 | citrate | - | carbon source |
121823 | 4853 | esculin | - | hydrolysis |
121823 | 606565 | hippurate | + | hydrolysis |
121823 | 17632 | nitrate | - | reduction |
121823 | 16301 | nitrite | - | reduction |
121823 | 17632 | nitrate | - | respiration |
metabolite production
- @ref: 121823
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
121823 | 15688 | acetoin | - | |
121823 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23030 | catalase | + | 1.11.1.6 |
23030 | cytochrome oxidase | + | 1.9.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
121823 | oxidase | - | |
121823 | beta-galactosidase | + | 3.2.1.23 |
121823 | alcohol dehydrogenase | - | 1.1.1.1 |
121823 | gelatinase | +/- | |
121823 | amylase | + | |
121823 | DNase | - | |
121823 | caseinase | - | 3.4.21.50 |
121823 | catalase | + | 1.11.1.6 |
121823 | tween esterase | + | |
121823 | gamma-glutamyltransferase | + | 2.3.2.2 |
121823 | lysine decarboxylase | - | 4.1.1.18 |
121823 | ornithine decarboxylase | - | 4.1.1.17 |
121823 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121823 | - | + | + | - | - | - | - | - | - | - | + | + | - | + | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
5586 | mural painting | Seville, Carmona | Spain | ESP | Europe |
60324 | Mural painting | Spain | ESP | Europe | |
67771 | From mural painting | Carmona, Seville | Spain | ESP | Europe |
121823 | Mural painting | Spain | ESP | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Built environment | |
#Engineered | #Other | #Painting |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5586 | 1 | Risk group (German classification) |
121823 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Oceanobacillus picturae genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 637 | AB367167 | 637 | ena | 171693 |
20218 | Oceanobacillus picturae genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 484 | AB367168 | 484 | ena | 171693 |
20218 | Virgibacillus picturae 16S rRNA gene, type strain LMG 19492T | AJ315060 | 1516 | ena | 171693 |
20218 | Virgibacillus picturae partial 16S rRNA gene, isolate OS-221.b | AM237397 | 1468 | ena | 171693 |
GC content
- @ref: 67771
- GC-content: 39.5
External links
@ref: 5586
culture collection no.: DSM 14867, LMG 19492, CCUG 54775, KCTC 3821, CIP 108264
straininfo link
- @ref: 71024
- straininfo: 13182
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12710619 | Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. | Heyrman J, Logan NA, Busse HJ, Balcaen A, Lebbe L, Rodriguez-Diaz M, Swings J, De Vos P | Int J Syst Evol Microbiol | 10.1099/ijs.0.02371-0 | 2003 | Bacillus/*classification/genetics/isolation & purification/metabolism, Base Composition, Biodegradation, Environmental, Biofilms, DNA, Bacterial/analysis/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Paintings, Phenotype, Phylogeny, RNA, Ribosomal, 16S/*analysis/genetics, Sequence Analysis, DNA | Genetics |
Phylogeny | 19620352 | Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. | Namwong S, Tanasupawat S, Lee KC, Lee JS | Int J Syst Evol Microbiol | 10.1099/ijs.0.007161-0 | 2009 | Bacillaceae/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, *Food Microbiology, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Thailand, Vitamin K 2/analysis | Biotechnology |
Phylogeny | 23625258 | Oceanobacillus chungangensis sp. nov., isolated from a sand dune. | Lee DC, Kang H, Weerawongwiwat V, Kim B, Choi YW, Kim W | Int J Syst Evol Microbiol | 10.1099/ijs.0.046441-0 | 2013 | Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Silicon Dioxide, Vitamin K 2/analogs & derivatives/analysis | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5586 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14867) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14867 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23030 | Jung-Sook Lee,Jee-Min Lim,Keun Chul Lee,Jae-Chan Lee,Yong-Ha Park,Chang-Jin Kim | 10.1099/ijs.0.63734-0 | Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions | IJSEM 56: 251-257 2006 | 16403894 | |
33218 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5867 | ||||
60324 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 54775) | https://www.ccug.se/strain?id=54775 | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
68382 | Automatically annotated from API zym | |||||
71024 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13182.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121823 | Curators of the CIP | Collection of Institut Pasteur (CIP 108264) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108264 |