Strain identifier

BacDive ID: 137298

Type strain: No

Species: Escherichia coli

Strain Designation: DH10B

Strain history: CIP <- 1998, M. Cohen-Salmon, Inst. Pasteur, Paris, France: strain DH10B

NCBI tax ID(s): 562 (species)

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General

@ref: 37213

BacDive-ID: 137298

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Escherichia coli DH10B is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 37213
  • history: CIP <- 1998, M. Cohen-Salmon, Inst. Pasteur, Paris, France: strain DH10B

doi: 10.13145/bacdive137298.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 37213

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

strain designation: DH10B

type strain: no

Morphology

cell morphology

  • @ref: 37213
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 37213

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37213MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
37213CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
37213CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
37213positivegrowth30mesophilic
37213positivegrowth30-41
37213nogrowth5psychrophilic
37213nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 37213
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3721329864mannitol+fermentation
3721316947citrate-carbon source
372134853esculin+hydrolysis
3721317234glucose+fermentation
3721317716lactose-fermentation
3721317632nitrate+reduction
3721316301nitrite-reduction
3721315792malonate-assimilation
37213132112sodium thiosulfate-builds gas from
3721317234glucose+degradation

antibiotic resistance

  • @ref: 37213
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 37213
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3721315688acetoin-
3721317234glucose+

enzymes

@refvalueactivityec
37213oxidase-
37213beta-galactosidase-3.2.1.23
37213alcohol dehydrogenase-1.1.1.1
37213gelatinase-
37213catalase+1.11.1.6
37213lysine decarboxylase+4.1.1.18
37213ornithine decarboxylase-4.1.1.17
37213phenylalanine ammonia-lyase-4.3.1.24
37213tryptophan deaminase-
37213urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
37213-++--+----+++-++----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
37213---------------------------------------------------------------------------------------------------

Safety information

risk assessment

  • @ref: 37213
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 37213

culture collection no.: CIP 105601

straininfo link

  • @ref: 94233
  • straininfo: 68394

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
37213Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105601Collection of Institut Pasteur (CIP 105601)
68382Automatically annotated from API zym
94233Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID68394.1