Strain identifier

BacDive ID: 137233

Type strain: No

Species: Klebsiella pneumoniae subsp. ozaenae

Strain Designation: F5052, O30

Strain history: CIP <- 1952, I. Oerskov, Copenhagen, Denmark: strain F5052 <- E.P. Snijders, Amsterdam, The Netherlands: strain O30

NCBI tax ID(s): 574 (subspecies)

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General

@ref: 37120

BacDive-ID: 137233

keywords: genome sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative

description: Klebsiella pneumoniae subsp. ozaenae F5052 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 574
  • Matching level: subspecies

strain history

  • @ref: 37120
  • history: CIP <- 1952, I. Oerskov, Copenhagen, Denmark: strain F5052 <- E.P. Snijders, Amsterdam, The Netherlands: strain O30

doi: 10.13145/bacdive137233.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae subsp. ozaenae
  • full scientific name: Klebsiella pneumoniae subsp. ozaenae (Abel 1893) Ørskov 1984

  • synonyms

    @refsynonym
    20215Bacillus ozaenae
    20215Klebsiella ozaenae

@ref: 37120

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae subsp. ozaenae

strain designation: F5052, O30

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
125439negative95.5
125438negative100
37120negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37120MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
37120CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
37120CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
37120positivegrowth30
37120positivegrowth30-37
37120nogrowth5
37120nogrowth10
37120nogrowth41

Physiology and metabolism

oxygen tolerance

  • @ref: 37120
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 90.8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3712029864mannitol+fermentation
3712016947citrate+carbon source
3712017234glucose+fermentation
3712017716lactose-fermentation
3712017632nitrate+reduction
3712016301nitrite-reduction
3712015792malonate-assimilation
37120132112sodium thiosulfate-builds gas from
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837112936D-galactose+builds acid from
68371Potassium 2-ketogluconate+builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose+builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837128053melibiose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol+builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
6836817632nitrate-reduction
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

antibiotic resistance

  • @ref: 37120
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
3712035581indoleno
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
3712015688acetoin-
3712017234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
37120oxidase-
37120beta-galactosidase+3.2.1.23
37120alcohol dehydrogenase-1.1.1.1
37120gelatinase-
37120catalase+1.11.1.6
37120lysine decarboxylase-4.1.1.18
37120ornithine decarboxylase-4.1.1.17
37120phenylalanine ammonia-lyase-4.3.1.24
37120tryptophan deaminase-
37120urease-3.5.1.5
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368arginine dihydrolase-3.5.3.6
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382acid phosphatase+3.1.3.2
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368lysine decarboxylase-4.1.1.18
68368beta-galactosidase-3.2.1.23

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
37120-+++-+----++++-+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUINOSorRHASACMELNO2N2
37120-------------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
37120+--+++-+-++++-+-+++-++-++++++/-++/-+--++/---++/----++-++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
37120+++++++-+++++++++++++++++-+----+++++++---+-+++---++--++++--+++-----------+-+--+++++------++++++---+

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentisolation date
37120AmsterdamNetherlandsNLDEurope
37120AmsterdamNetherlandsNLDEurope1937

Safety information

risk assessment

  • @ref: 37120
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Klebsiella pneumoniae NCTC5052GCA_900451765contigncbi573
66792Klebsiella pneumoniae strain NCTC5052573.20138wgspatric573

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno100no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no93.229yes
125438spore-formingspore-formingAbility to form endo- or exosporesno89.829no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no60.099yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno98yes
125438motile2+flagellatedAbility to perform flagellated movementno77.13no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno90.8
125439BacteriaNetmotilityAbility to perform movementyes71
125439BacteriaNetgram_stainReaction to gram-stainingnegative95.5
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthaerobe71

External links

@ref: 37120

culture collection no.: CIP 52.213, ATCC 11298, NCTC 5052

straininfo link

  • @ref: 94180
  • straininfo: 35341

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
37120Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.213Collection of Institut Pasteur (CIP 52.213)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
94180Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID35341.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG