Strain identifier

BacDive ID: 1372

Type strain: Yes

Species: Oceanobacillus iheyensis

Strain Designation: HTE831

Strain history: CIP <- 2002, JCM <- 2001, H. Takami: strain HTE831

NCBI tax ID(s): 182710 (species)

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General

@ref: 5326

BacDive-ID: 1372

DSM-Number: 14371

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, motile, rod-shaped

description: Oceanobacillus iheyensis HTE831 is a mesophilic, Gram-positive, motile bacterium that was isolated from deep sea sediment, 1050 m depth.

NCBI tax id

  • NCBI tax id: 182710
  • Matching level: species

strain history

@refhistory
5326<- H. Takami; HTE831
67770H. Takami HTE831.
120046CIP <- 2002, JCM <- 2001, H. Takami: strain HTE831

doi: 10.13145/bacdive1372.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oceanobacillus
  • species: Oceanobacillus iheyensis
  • full scientific name: Oceanobacillus iheyensis Lu et al. 2002

@ref: 5326

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Oceanobacillus

species: Oceanobacillus iheyensis

full scientific name: Oceanobacillus iheyensis Lu et al. 2002

strain designation: HTE831

type strain: yes

Morphology

cell morphology

  • @ref: 120046
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 120046

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5326BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
37958MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120046CIP Medium 648yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=648
120046CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5326positivegrowth28mesophilic
37958positivegrowth30mesophilic
67770positivegrowth30mesophilic
120046positivegrowth22-37
120046nogrowth10psychrophilic
120046nogrowth45thermophilic

culture pH

  • @ref: 120046
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
120046NaClpositivegrowth4-10 %
120046NaClnogrowth0 %
120046NaClnogrowth2 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12004616947citrate-carbon source
1200464853esculin-hydrolysis
120046606565hippurate+hydrolysis
12004617632nitrate-reduction
12004616301nitrite-reduction

metabolite production

  • @ref: 120046
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12004615688acetoin-
12004617234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120046oxidase-
120046beta-galactosidase-3.2.1.23
120046alcohol dehydrogenase-1.1.1.1
120046gelatinase-
120046amylase-
120046DNase-
120046caseinase-3.4.21.50
120046catalase+1.11.1.6
120046tween esterase-
120046gamma-glutamyltransferase+2.3.2.2
120046lecithinase-
120046lipase-
120046lysine decarboxylase-4.1.1.18
120046ornithine decarboxylase-4.1.1.17
120046protease-
120046urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120046--++-+-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120046+--------++----++-+----+-----------+-----------------+------+-------------------+-------+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5326deep sea sediment, 1050 m depthNanse,Okinawa, Iheya RidgeJapanJPNAsia
67770Deep-sea mud sample collected at a depth of 1,050 m on the Iheya Ridge of the Nansei Islands
120046Environment, Deep sea mudSamples collected from the Iheya Ridge of the Nansei Islands (1050 m deep) by means of the manned submersible Shinkai 2000 ((Takagawa et al. 1989)JapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_821.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_498;97_570;98_655;99_821&stattab=map
  • Last taxonomy: Oceanobacillus
  • 16S sequence: AB010863
  • Sequence Identity:
  • Total samples: 5256
  • soil counts: 2801
  • aquatic counts: 673
  • animal counts: 830
  • plant counts: 952

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53261Risk group (German classification)
1200461Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5326
  • description: Oceanobacillus iheyensis HTE831 gene for 16S ribosomal RNA, partial sequence
  • accession: AB010863
  • length: 1525
  • database: ena
  • NCBI tax ID: 221109

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Oceanobacillus iheyensis HTE831GCA_000011245completencbi221109
66792Oceanobacillus iheyensis HTE831221109.4completepatric221109
66792Oceanobacillus iheyensis HTE831637000201completeimg221109

GC content

@refGC-contentmethod
532635.8
6777035.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes86.252no
flagellatedyes84.798no
gram-positiveyes94.077no
anaerobicno99.449no
halophileyes92.543no
spore-formingyes95.287no
thermophileno99.503yes
glucose-utilyes88.523no
aerobicyes95.297no
glucose-fermentno86.87no

External links

@ref: 5326

culture collection no.: DSM 14371, JCM 11309, CGMCC 1.8876, CIP 107618, KCTC 3954

straininfo link

  • @ref: 71023
  • straininfo: 100830

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11750818Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge.Lu J, Nogi Y, Takami HFEMS Microbiol Lett10.1111/j.1574-6968.2001.tb10963.x2001Base Composition, Culture Media, Genome, Bacterial, Gram-Negative Aerobic Rods and Cocci/*classification/genetics, Halobacterium, Hydrogen-Ion Concentration, Japan, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sodium Chloride, Species Specificity, Spores, Temperature, *Water MicrobiologyGenetics
Genetics12235376Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments.Takami H, Takaki Y, Uchiyama INucleic Acids Res10.1093/nar/gkf5262002Bacillus/genetics/metabolism, Bacillus subtilis/genetics/metabolism, Bacteria/*genetics/metabolism, Bacterial Proteins/genetics, Base Composition, Biological Transport, Chromosomes, Bacterial/genetics, DNA, Bacterial/chemistry/*genetics, DNA, Circular/genetics, Genes, Bacterial/genetics, *Genome, Bacterial, Molecular Sequence Data, Oceans and Seas, Sequence Analysis, DNA, Sodium/pharmacokinetics, Species Specificity, Water MicrobiologyMetabolism
Genetics15500249Identification and distribution of new insertion sequences in the genome of the extremely halotolerant and alkaliphilic Oceanobacillus iheyensis HTE831.Takaki Y, Matsuki A, Chee GJ, Takami HDNA Res10.1093/dnares/11.4.2332004Amino Acid Sequence, Bacillus/classification/genetics, Bacterial Proteins/genetics, Base Sequence, DNA Transposable Elements/*genetics, DNA, Bacterial/*genetics, Gene Duplication, Genome, Bacterial, Halobacteriales/*genetics/physiology, Introns, Molecular Sequence Data, Nucleic Acid Conformation, Open Reading Frames, RNA, Bacterial/genetics, RNA, Catalytic/genetics, RNA-Directed DNA Polymerase/genetics, Sequence Alignment, Sequence Homology, Species Specificity, Spores, Bacterial, Transposases/genetics, Vancomycin Resistance/geneticsPhylogeny
Metabolism16233841D-Amino acid dipeptide production utilizing D-alanine-D-alanine ligases with novel substrate specificity.Sato M, Kirimura K, Kino KJ Biosci Bioeng10.1263/jbb.99.6232005Amino Acid Sequence, Cloning, Molecular, Dipeptides/*chemistry/*metabolism, Enzyme Activation, Escherichia coli/genetics/*metabolism, Molecular Sequence Data, Peptide Synthases/*chemistry/genetics/*metabolism, Protein Engineering/*methods, Recombinant Proteins/metabolism, Stereoisomerism, Substrate SpecificityEnzymology
Phylogeny16585699Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi.Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A, Giordano AInt J Syst Evol Microbiol10.1099/ijs.0.63575-02006Bacillaceae/*classification/genetics/*isolation & purification, Eukaryota/growth & development/*microbiology, Fresh Water/chemistry/*microbiology, Lipids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/metabolism, Sulfur/*metabolismGenetics
Metabolism17146838Multidimensional analysis of the insoluble sub-proteome of Oceanobacillus iheyensis HTE831, an alkaliphilic and halotolerant deep-sea bacterium isolated from the Iheya ridge.Graham RL, Pollock CE, O'Loughlin SN, Ternan NG, Weatherly DB, Tarleton RL, McMullan GProteomics10.1002/pmic.2006006652007Amino Acid Sequence, Bacillaceae/*metabolism, Bacterial Proteins/*metabolism, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Proteome/*metabolism, Spectrometry, Mass, Electrospray IonizationProteome
Phylogeny17267960Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass.Raats D, Halpern MInt J Syst Evol Microbiol10.1099/ijs.0.64502-02007Animals, Bacillaceae/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Chironomidae/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis/chemistry, Genes, rRNA/genetics, Hydrogen-Ion Concentration, Israel, Molecular Sequence Data, Molecular Weight, Movement, Ovum/*microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride, TemperatureGenetics
Genetics17993671CGAS: a comparative genome annotation system.Choi K, Yang Y, Kim SMethods Mol Biol1-59745-514-8:1332007*Databases, Genetic, *Genome, Internet, Open Reading Frames
Enzymology19269582Characterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4.Ju J, Xu S, Wen J, Li G, Ohnishi K, Xue Y, Ma YJ Biosci Bioeng10.1016/j.jbiosc.2008.11.0052009Alanine/metabolism, Alanine Racemase/isolation & purification/*metabolism, Amino Acid Sequence, Bacillus/*enzymology, Bacterial Proteins/isolation & purification/*metabolism, Cloning, Molecular, Hydrogen-Ion Concentration, Molecular Sequence Data, Pyridoxal Phosphate/*metabolism, Substrate Specificity, TemperatureMetabolism
Phylogeny19946056Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor.Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CSInt J Syst Evol Microbiol10.1099/ijs.0.016295-02009Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Esculin/metabolism, Fatty Acids/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Transmission, Molecular Sequence Data, Oxidoreductases/metabolism, Phylogeny, Pigments, Biological/biosynthesis, Polysorbates/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Sodium Chloride/metabolismMetabolism
Metabolism23451075Biochemical and mutational analysis of a novel nicotinamidase from Oceanobacillus iheyensis HTE831.Sanchez-Carron G, Garcia-Garcia MI, Zapata-Perez R, Takami H, Garcia-Carmona F, Sanchez-Ferrer APLoS One10.1371/journal.pone.00567272013Bacillaceae/*enzymology/genetics, Niacinamide/metabolism, Nicotinamidase/classification/genetics/*metabolism, Phylogeny, Pyrazinamide/metabolism, Substrate SpecificityPhylogeny
Phylogeny24425745Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment.Yu C, Yu S, Zhang Z, Li Z, Zhang XHInt J Syst Evol Microbiol10.1099/ijs.0.056481-02014Bacillaceae/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Molecular Sequence Data, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitle
5326Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14371)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14371
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37958Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5074
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71023Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100830.1StrainInfo: A central database for resolving microbial strain identifiers
120046Curators of the CIPCollection of Institut Pasteur (CIP 107618)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107618