Strain identifier

BacDive ID: 137170

Type strain: No

Species: Legionella pneumophila

Strain Designation: 82A3105

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- D. Lindquist: strain 82A3105 <- S. Paula

NCBI tax ID(s): 446 (species)

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General

@ref: 37038

BacDive-ID: 137170

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella pneumophila 82A3105 is a microaerophile, mesophilic, Gram-negative bacterium of the family Legionellaceae.

NCBI tax id

  • NCBI tax id: 446
  • Matching level: species

strain history

  • @ref: 37038
  • history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- D. Lindquist: strain 82A3105 <- S. Paula

doi: 10.13145/bacdive137170.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila
  • full scientific name: Legionella pneumophila Brenner et al. 1979 (Approved Lists 1980)

@ref: 37038

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila

strain designation: 82A3105

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
37038negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37038MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
37038CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
37038positivegrowth37mesophilic
56535positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 56535
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.992

enzymes

@refvalueactivityec
37038oxidase-
37038catalase-1.11.1.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • @reffatty acidpercentageECL
    56535C14:00.614
    56535C15:0215
    56535C16:05.516
    56535C17:0117
    56535C18:01.318
    56535C20:00.720
    56535C14:0 ISO7.213.618
    56535C14:1 ω5c1.813.899
    56535C15:0 3OH0.516.504
    56535C15:0 ANTEISO12.614.711
    56535C15:0 ISO0.514.621
    56535C15:0 ISO 3OH1.316.135
    56535C15:1 ω6c3.614.856
    56535C16:0 iso27.415.626
    56535C16:1 ISO G4.615.442
    56535C16:1 ω7c21.515.819
    56535C17:0 anteiso4.316.722
    56535C17:0 CYCLO1.516.888
    56535Unidentified2.115.925
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    56535C14:00.514
    56535C15:0115
    56535C16:04.516
    56535C17:00.717
    56535C18:00.718
    56535C14:0 ISO8.413.618
    56535C14:0 ISO 3OH0.715.117
    56535C14:1 ω5c0.813.899
    56535C15:0 ANTEISO14.414.711
    56535C15:0 ISO0.714.621
    56535C15:1 ω6c4.314.856
    56535C16:0 iso27.615.626
    56535C16:1 ISO H6.215.461
    56535C16:1 ω7c24.115.819
    56535C17:0 anteiso3.416.722
    56535C17:0 CYCLO116.888
    56535C17:1 ω9c ANTEISO0.716.525
    56535Unidentified0.5
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
37038-+++-++-+-++--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
37038CaliforniaUSAUSANorth America
50201CaliforniaUSAUSANorth AmericaLung aspirate
56535CaliforniaUSAUSANorth AmericaHuman lung aspirate
37038CaliforniaUnited States of AmericaUSANorth AmericaHuman, Lung aspirate

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung
#Host Body Product#Fluids#Aspirate

Safety information

risk assessment

  • @ref: 37038
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella pneumophila subsp. pneumophila ATCC 43736GCA_000586075scaffoldncbi91891
66792Legionella pneumophila subsp. pneumophila strain ATCC 4373691891.24wgspatric91891
66792Legionella pneumophila pneumophila ATCC 437362907337367draftimg91891

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes78.352no
flagellatedno74.331no
gram-positiveno98.429no
anaerobicno98.718yes
halophileno85.993no
spore-formingno94.724no
glucose-utilyes67.96no
aerobicno90.865yes
thermophileno98.274yes
glucose-fermentno85.687no

External links

@ref: 37038

culture collection no.: CIP 103867, ATCC 43736, CCUG 44897, CCUG 30662

straininfo link

  • @ref: 94126
  • straininfo: 42013

literature

  • topic: Pathogenicity
  • Pubmed-ID: 3356793
  • title: Thirteenth serogroup of Legionella pneumophila isolated from patients with pneumonia.
  • authors: Lindquist DS, Nygaard G, Thacker WL, Benson RF, Brenner DJ, Wilkinson HW
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.26.3.586-587.1988
  • year: 1988
  • mesh: Adult, Aged, Female, Humans, Legionella/*classification/isolation & purification, Legionnaires' Disease/*microbiology, Male, Middle Aged, Serotyping
  • topic2: Phylogeny

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
37038Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103867Collection of Institut Pasteur (CIP 103867)
50201Curators of the CCUGhttps://www.ccug.se/strain?id=30662Culture Collection University of Gothenburg (CCUG) (CCUG 30662)
56535Curators of the CCUGhttps://www.ccug.se/strain?id=44897Culture Collection University of Gothenburg (CCUG) (CCUG 44897)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
94126Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID42013.1