Strain identifier

BacDive ID: 137152

Type strain: No

Species: Shigella flexneri

Strain Designation: 24 D

Strain history: CIP <- 1952, Dysentery Ref. Lab., Oxford, UK: strain 24 D

NCBI tax ID(s): 623 (species)

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General

@ref: 37016

BacDive-ID: 137152

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Shigella flexneri 24 D is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 623
  • Matching level: species

strain history

@refhistory
370161952, Dysentery Ref. Lab., Oxford, UK: strain 24 D
37016CIP <- 1952, Dysentery Ref. Lab., Oxford, UK: strain 24 D

doi: 10.13145/bacdive137152.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Shigella
  • species: Shigella flexneri
  • full scientific name: Shigella flexneri Castellani and Chalmers 1919 (Approved Lists 1980)

@ref: 37016

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Shigella

species: Shigella flexneri

strain designation: 24 D

type strain: no

Morphology

cell morphology

  • @ref: 37016
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37016MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
37016CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
37016CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
37016positivegrowth30mesophilic
37016positivegrowth30-41
37016nogrowth5psychrophilic
37016nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 37016
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3701629864mannitol+fermentation
3701616947citrate-carbon source
3701617234glucose+fermentation
3701617716lactose-fermentation
3701617632nitrate+reduction
3701616301nitrite-reduction
3701615792malonate-assimilation
37016132112sodium thiosulfate-builds gas from
3701617234glucose+degradation

antibiotic resistance

  • @ref: 37016
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 37016
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3701615688acetoin-
3701617234glucose+

enzymes

@refvalueactivityec
37016oxidase-
37016beta-galactosidase-3.2.1.23
37016alcohol dehydrogenase-1.1.1.1
37016gelatinase-
37016catalase+1.11.1.6
37016lysine decarboxylase-4.1.1.18
37016ornithine decarboxylase-4.1.1.17
37016phenylalanine ammonia-lyase-4.3.1.24
37016tryptophan deaminase-
37016urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
37016-+---++-+-++---+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
37016+++++-+-+++---------+----------+-+--------------------++----+--------------+-----------------+-----

Safety information

risk assessment

  • @ref: 37016
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 37016

culture collection no.: CIP 52.36

straininfo link

  • @ref: 94114
  • straininfo: 70176

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
37016Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.36Collection of Institut Pasteur (CIP 52.36)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
94114Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID70176.1