Strain identifier

BacDive ID: 137068

Type strain: No

Species: Lactobacillus crispatus

Strain Designation: 58-098-135

Strain history: CIP <- 1995, F. Gasser, Inst. Pasteur, Paris, France: strain 63Fa, Lactobacillus acidophilus <- F. Guillermet, Inst. Pasteur, Lyon, France: strain 58-098-135 <- Croix-Rousse Hosp., Lyon, France

NCBI tax ID(s): 47770 (species)

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General

@ref: 36916

BacDive-ID: 137068

keywords: genome sequence, Bacteria, facultative anaerobe, Gram-positive

description: Lactobacillus crispatus 58-098-135 is a facultative anaerobe, Gram-positive bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 47770
  • Matching level: species

strain history

  • @ref: 36916
  • history: CIP <- 1995, F. Gasser, Inst. Pasteur, Paris, France: strain 63Fa, Lactobacillus acidophilus <- F. Guillermet, Inst. Pasteur, Lyon, France: strain 58-098-135 <- Croix-Rousse Hosp., Lyon, France

doi: 10.13145/bacdive137068.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus crispatus
  • full scientific name: Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Eubacterium crispatum

@ref: 36916

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus crispatus

strain designation: 58-098-135

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.5
6948094.193positive
36916nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36916MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
36916CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
36916positivegrowth37
36916nogrowth15
36916nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 36916
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 91.438

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
36916nitrate-reduction17632
36916nitrite-reduction16301
36916nitrate+respiration17632

metabolite tests

  • @ref: 36916
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
36916oxidase-
36916alcohol dehydrogenase-1.1.1.1
36916catalase-1.11.1.6
36916lysine decarboxylase-4.1.1.18
36916ornithine decarboxylase-4.1.1.17

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
36916---------++++--------++/-++++++-++---++-+/-----------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
36916LyonFranceFRAEurope
36916LyonFranceFRAEuropeHuman, Vaginal swab1955

Safety information

risk assessment

  • @ref: 36916
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus crispatus CIP 104459GCA_008079315contigncbi47770
66792Lactobacillus crispatus strain CIP 10445947770.572wgspatric47770

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes94.193no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no69.032no
69480spore-formingspore-formingAbility to form endo- or exosporesno91.438no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.643yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.5no
69480flagellatedmotile2+Ability to perform flagellated movementno93.5no

External links

@ref: 36916

culture collection no.: CIP 104459

straininfo link

  • @ref: 94043
  • straininfo: 68990

literature

topicPubmed-IDtitleauthorsjournalDOIyeartopic2mesh
Genetics31919178Draft Genome Sequence of Lactobacillus crispatus CIP 104459, Isolated from a Vaginal Swab.Clabaut M, Boukerb AM, Racine PJ, Pichon C, Kremser C, Picot JP, Karsybayeva M, Redziniak G, Chevalier S, Feuilloley MGJMicrobiol Resour Announc10.1128/MRA.01373-192020Phylogeny
Pathogenicity34075148Variability of the response of human vaginal Lactobacillus crispatus to 17beta-estradiol.Clabaut M, Boukerb AM, Mlouka AB, Suet A, Tahrioui A, Verdon J, Barreau M, Maillot O, Le Tirant A, Karsybayeva M, Kremser C, Redziniak G, Duclairoir-Poc C, Pichon C, Hardouin J, Cosette P, Chevalier S, Feuilloley MGJSci Rep10.1038/s41598-021-91017-52021EnzymologyEstradiol/*pharmacology, Female, Humans, Lactobacillus crispatus/isolation & purification/*metabolism, Vagina/*microbiology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36916Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104459Collection of Institut Pasteur (CIP 104459)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
94043Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID68990.1