Strain identifier

BacDive ID: 137038

Type strain: Yes

Species: Helicobacter mustelae

Strain Designation: R85-136P

Strain history: CIP <- 1996, ATCC <- 1989, NCTC <- ATCC <- J.G. Fox, Cambridge, MA, USA: strain R85-136P

NCBI tax ID(s): 217 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 8.1 (current version)

General

@ref: 36872

BacDive-ID: 137038

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Helicobacter mustelae R85-136P is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from Ferret gastric mucosa.

NCBI tax id

  • NCBI tax id: 217
  • Matching level: species

strain history

  • @ref: 36872
  • history: CIP <- 1996, ATCC <- 1989, NCTC <- ATCC <- J.G. Fox, Cambridge, MA, USA: strain R85-136P

doi: 10.13145/bacdive137038.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Helicobacteraceae
  • genus: Helicobacter
  • species: Helicobacter mustelae
  • full scientific name: Helicobacter mustelae (Fox et al. 1988) Goodwin et al. 1989
  • synonyms

    @refsynonym
    20215Campylobacter mustelae
    20215Campylobacter pylori subsp. mustelae

@ref: 36872

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Helicobacteraceae

genus: Helicobacter

species: Helicobacter mustelae

strain designation: R85-136P

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.998
36872negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36872MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
36872CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
36872positivegrowth37mesophilic
48251positivegrowth42thermophilic
36872positivegrowth41thermophilic
36872nogrowth25mesophilic
36872nogrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 48251
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.996

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
36872606565hippurate-hydrolysis
3687217632nitrate+reduction
3687216301nitrite-reduction

metabolite production

  • @ref: 36872
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
36872oxidase+
36872catalase+1.11.1.6
36872urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
36872-+++-+----++---+----

Isolation, sampling and environmental information

isolation

@refsample type
48251Ferret gastric mucosa
36872Animal, Ferret, gastric mucosa

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Gastrointestinal tract
  • Cat3: #Stomach

Safety information

risk assessment

  • @ref: 36872
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Helicobacter mustelae NCTC12198GCA_900476215completencbi217
66792Helicobacter mustelae strain NCTC12198217.6completepatric217
66792Helicobacter mustelae ATCC 43772646564537completeimg679897
66792Helicobacter mustelae NCTC 121982808606644completeimg217

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes59.279no
gram-positiveno98.953no
anaerobicno90.172no
aerobicno96.24no
halophileno81.419no
spore-formingno97.267no
thermophileno63.216no
glucose-utilno78.773no
motileyes89.22no
glucose-fermentno85.105no

External links

@ref: 36872

culture collection no.: CIP 103759, ATCC 43772, CCUG 25715, NCTC 12198

straininfo link

  • @ref: 94020
  • straininfo: 7400

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopic
9125590Infection of the ferret stomach by isogenic flagellar mutant strains of Helicobacter mustelae.Andrutis KA, Fox JG, Schauer DB, Marini RP, Li X, Yan L, Josenhans C, Suerbaum SInfect Immun10.1128/iai.65.5.1962-1966.19971997Animals, Antibodies, Bacterial/analysis, Biopsy, Colony Count, Microbial, Female, Ferrets, Flagellin/*genetics, Gastric Mucosa/microbiology, Helicobacter/*genetics/growth & development/immunology, Helicobacter Infections/*genetics/immunology/*microbiology, Male, Specific Pathogen-Free Organisms, Stomach/*microbiology/pathology
9297827The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 expresses the monofucosyl A type 1 histo-blood group epitope.Monteiro MA, Zheng PY, Appelmelk BJ, Perry MBFEMS Microbiol Lett10.1111/j.1574-6968.1997.tb12630.x1997Animals, Bacterial Outer Membrane Proteins/chemistry/immunology, Carbohydrate Sequence, Epitopes/*analysis, Ferrets, Fucose, Gastric Mucosa/microbiology, Gastritis/microbiology, Helicobacter/*chemistry/immunology, Lipopolysaccharides/*chemistry/immunology, Magnetic Resonance Spectroscopy, Molecular Sequence DataGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36872Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103759Collection of Institut Pasteur (CIP 103759)
48251Curators of the CCUGhttps://www.ccug.se/strain?id=25715Culture Collection University of Gothenburg (CCUG) (CCUG 25715)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
94020Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID7400.1