Strain identifier

BacDive ID: 136808

Type strain: No

Species: Achromobacter xylosoxidans

Strain Designation: 325

Strain history: CIP <- 1962, R.C. Cleverdon, Connecticut Univ., USA: strain 325

NCBI tax ID(s): 85698 (species)

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General

@ref: 36460

BacDive-ID: 136808

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile

description: Achromobacter xylosoxidans 325 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Alcaligenaceae.

NCBI tax id

  • NCBI tax id: 85698
  • Matching level: species

strain history

@refhistory
364601962, R.C. Cleverdon, Connecticut Univ., USA: strain 325
67770IAM 12596 <-- CIP 62.30 <-- P. Thibault <-- R. C. Cleverdon 325 (Alcaligenes faecalis).
36460CIP <- 1962, R.C. Cleverdon, Connecticut Univ., USA: strain 325

doi: 10.13145/bacdive136808.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Achromobacter
  • species: Achromobacter xylosoxidans
  • full scientific name: Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971) Yabuuchi and Yano 1981
  • synonyms

    @refsynonym
    20215Achromobacter xylosoxidans
    20215Alcaligenes xylosoxidans
    20215Alcaligenes xylosoxydans
    20215Alcaligenes denitrificans subsp. xylosoxydans

@ref: 36460

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Achromobacter

species: Achromobacter xylosoxidans

strain designation: 325

type strain: no

Morphology

cell morphology

  • @ref: 36460
  • gram stain: negative
  • motility: yes

colony morphology

  • @ref: 36460

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36460MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
36460CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
36460positivegrowth30mesophilic
67770positivegrowth30mesophilic
36460positivegrowth15-45
36460nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 36460
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
36460NaClpositivegrowth0-6 %
36460NaClnogrowth8 %
36460NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3646016947citrate+carbon source
364604853esculin-hydrolysis
36460606565hippurate-hydrolysis
3646017632nitrate+builds gas from
3646017632nitrate+reduction
3646016301nitrite+builds gas from
3646016301nitrite+reduction
3646015792malonate-assimilation
3646017632nitrate+respiration

antibiotic resistance

  • @ref: 36460
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 36460
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3646015688acetoin-
3646017234glucose-

enzymes

@refvalueactivityec
36460oxidase+
36460beta-galactosidase-3.2.1.23
36460alcohol dehydrogenase-1.1.1.1
36460gelatinase-
36460amylase-
36460DNase-
36460caseinase-3.4.21.50
36460catalase+1.11.1.6
36460tween esterase-
36460gamma-glutamyltransferase-2.3.2.2
36460lecithinase-
36460lipase-
36460lysine decarboxylase-4.1.1.18
36460ornithine decarboxylase-4.1.1.17
36460phenylalanine ammonia-lyase-4.3.1.24
36460tryptophan deaminase-
36460urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
36460-++--+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
36460+---+----------------+---------------------++-++++++++----+-++---++------+-+++++++++--+++++++++-+++

Safety information

risk assessment

  • @ref: 36460
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 36460

culture collection no.: CIP 62.30, JCM 20673, IAM 12596, LMG 3414

straininfo link

  • @ref: 93863
  • straininfo: 819

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36460Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2062.30Collection of Institut Pasteur (CIP 62.30)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
93863Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID819.1