Strain identifier

BacDive ID: 136791

Type strain: Yes

Species: Hymenobacter flocculans

Strain Designation: A2-50, A2-50A

Strain history: CIP <- 2010, P. Morais, FCTUC, Da Vida, Coimbra, Portugal : strain A2-50A

NCBI tax ID(s): 759411 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.2 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 9.2 (current version)

General

@ref: 36431

BacDive-ID: 136791

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Hymenobacter flocculans A2-50 is a mesophilic, Gram-negative, rod-shaped bacterium of the family Hymenobacteraceae.

NCBI tax id

  • NCBI tax id: 759411
  • Matching level: species

strain history

@refhistory
67772Chung A.P. University of Coimbra
36431CIP <- 2010, P. Morais, FCTUC, Da Vida, Coimbra, Portugal : strain A2-50A

doi: 10.13145/bacdive136791.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Hymenobacteraceae
  • genus: Hymenobacter
  • species: Hymenobacter flocculans
  • full scientific name: Hymenobacter flocculans Chung et al. 2011

  • synonyms

    • @ref: 20215
    • synonym: Siccationidurans flocculans

@ref: 36431

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Hymenobacteraceae

genus: Hymenobacter

species: Hymenobacter flocculans

strain designation: A2-50, A2-50A

type strain: yes

Morphology

cell morphology

  • @ref: 36431
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36431MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
36431CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperature
36431positivegrowth30
67772positiveminimum4
67772positiveoptimum25
67772positivemaximum40

culture pH

  • @ref: 67772
  • ability: positive
  • type: optimum
  • pH: 6.0-6.5

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3643117632nitrate-reduction
3643116301nitrite-reduction

metabolite production

  • @ref: 36431
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382valine arylamidase+
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
36431oxidase+
36431catalase+1.11.1.6
36431urease-3.5.1.5
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382leucine arylamidase+3.4.11.1
68382alkaline phosphatase+3.1.3.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
36431-+++-+++--++-+-++++-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typesampling dateisolation date
36431CentralPortugalPRTEurope
67772Viseu, UrgeiriçaPortugalPRTEuropeAcidic water sample (pH 5)2008
36431CentralPortugalPRTEuropeUranium, mine2008

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
677721
364311Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67772
  • description: Hymenobacter flocculans strain A2-50A 16S ribosomal RNA gene, partial sequence
  • accession: HM032897
  • length: 1501
  • database: nuccore
  • NCBI tax ID: 759411

External links

@ref: 36431

culture collection no.: CIP 110139, LMG 25699, UCCCB28

straininfo link

  • @ref: 93851
  • straininfo: 369526

literature

  • topic: Phylogeny
  • Pubmed-ID: 21051170
  • title: Hymenobacter perfusus sp. nov., Hymenobacter flocculans sp. nov. and Hymenobacter metalli sp. nov. three new species isolated from an uranium mine waste water treatment system.
  • authors: Chung AP, Lopes A, Nobre MF, Morais PV
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2010.09.002
  • year: 2010
  • mesh: Cytophagaceae/chemistry/*classification/isolation & purification/physiology, Hazardous Waste/analysis, Industrial Waste/analysis, Molecular Sequence Data, Phylogeny, Portugal, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*genetics, Random Amplified Polymorphic DNA Technique, Sequence Homology, *Uranium, *Water Microbiology
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36431Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110139Collection of Institut Pasteur (CIP 110139)
67772Curators of the UCCCBhttps://ucccb.uc.pt/
68382Automatically annotated from API zym
93851Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID369526.1