Strain identifier

BacDive ID: 136744

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: RON-3

Strain history: CIP <- 2000, P. Nordmann, Kremlin Bicêtre Hosp., Le Kremlin Bicêtre, France: strain RON-3 <- R. Poincaré Hosp., Garches, France

NCBI tax ID(s): 287 (species)

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General

@ref: 36282

BacDive-ID: 136744

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas aeruginosa RON-3 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 36282
  • history: CIP <- 2000, P. Nordmann, Kremlin Bicêtre Hosp., Le Kremlin Bicêtre, France: strain RON-3 <- R. Poincaré Hosp., Garches, France

doi: 10.13145/bacdive136744.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 36282

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

strain designation: RON-3

type strain: no

Morphology

cell morphology

  • @ref: 36282
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 36282
  • production: yes
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36282MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
36282CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
36282positivegrowth30mesophilic
36282positivegrowth10-41
36282nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 36282
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
36282NaClpositivegrowth0-6 %
36282NaClnogrowth8 %
36282NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3628216947citrate+carbon source
362824853esculin-hydrolysis
3628217632nitrate+reduction
3628216301nitrite+reduction
3628217632nitrate+respiration

antibiotic resistance

  • @ref: 36282
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 36282
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
36282oxidase+
36282beta-galactosidase-3.2.1.23
36282alcohol dehydrogenase+1.1.1.1
36282gelatinase+
36282amylase-
36282DNase-
36282caseinase+3.4.21.50
36282catalase+1.11.1.6
36282tween esterase+
36282lecithinase+
36282lipase+
36282lysine decarboxylase-4.1.1.18
36282ornithine decarboxylase-4.1.1.17
36282protease+
36282tryptophan deaminase-
36282urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
36282-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
36282++-+---------------+------+----+-+---------------++--+--+-+++-++++-+---++++++++++++++--++++++++++++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
36282GarchesFranceFRAEurope
36282GarchesFranceFRAEuropeHuman, Urine1998

Safety information

risk assessment

  • @ref: 36282
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 36282

culture collection no.: CIP 106817

straininfo link

  • @ref: 93812
  • straininfo: 144568

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36282Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106817Collection of Institut Pasteur (CIP 106817)
68382Automatically annotated from API zym
93812Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID144568.1