Strain identifier

BacDive ID: 13649

Type strain: Yes

Species: Ahrensia kielensis

Strain Designation: B9

Strain history: CIP <- 2001, D. Mazel, Inst. Pasteur, Paris, France <- 2001, IAM, Agrobacterium kielense <- ATCC <- R. Ahrens: strain B9

NCBI tax ID(s): 1120961 (strain), 76980 (species)

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General

@ref: 2348

BacDive-ID: 13649

DSM-Number: 5890

keywords: genome sequence, 16S sequence, Bacteria, Gram-negative

description: Ahrensia kielensis B9 is a Gram-negative bacterium that was isolated from brackish water.

NCBI tax id

NCBI tax idMatching level
1120961strain
76980species

strain history

@refhistory
2348<- ATCC <- R. Ahrens, B9
67770IAM 12618 <-- ATCC 25656 <-- R. Ahrens B9.
120412CIP <- 2001, D. Mazel, Inst. Pasteur, Paris, France <- 2001, IAM, Agrobacterium kielense <- ATCC <- R. Ahrens: strain B9

doi: 10.13145/bacdive13649.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Ahrensiaceae
  • genus: Ahrensia
  • species: Ahrensia kielensis
  • full scientific name: Ahrensia kielensis corrig. (ex Ahrens 1968) Uchino et al. 1999

  • synonyms

    • @ref: 20215
    • synonym: Ahrensia kieliense

@ref: 2348

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Ahrensiaceae

genus: Ahrensia

species: Ahrensia kielensis

full scientific name: Ahrensia kielensis (ex Ahrens 1968) Uchino et al. 1999 emend. Liu et al. 2016

strain designation: B9

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
120412negativerod-shapedyes
69480negative99.478

colony morphology

  • @ref: 120412

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_5890_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5890_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5890_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5890_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5890_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2348MARINE AGAR (DSMZ Medium 123)yeshttps://mediadive.dsmz.de/medium/123Name: MARINE AGAR (DSMZ Medium 123) Composition: NaCl 24.0 g/l Agar 15.0 g/l MgCl2 x 6 H2O 11.0 g/l Bacto peptone 5.0 g/l Na2SO4 4.0 g/l CaCl2 x 6 H2O 2.0 g/l Yeast extract 1.0 g/l KCl 0.7 g/l KBr 0.1 g/l SrCl2 x 6 H2O 0.04 g/l H3BO3 0.03 g/l NaSiO3 x 9 H2O 0.005 g/l NaF 0.003 g/l NH4NO3 0.002 g/l Fe(III)PO4 x 4 H2O 0.001 g/l Distilled water
39626Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120412CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperature
2348positivegrowth26
39626positivegrowth25
67770positivegrowth25
120412positivegrowth5-37
120412nogrowth41
120412nogrowth45

Physiology and metabolism

spore formation

@refspore formationconfidence
69480no92.849
69481no100

halophily

@refsaltgrowthtested relationconcentration
120412NaClpositivegrowth2-4 %
120412NaClnogrowth0 %
120412NaClnogrowth6 %
120412NaClnogrowth8 %
120412NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120412esculin-hydrolysis4853
120412hippurate-hydrolysis606565
120412nitrate-reduction17632
120412nitrite-reduction16301
120412malonate-assimilation15792
120412glucose+/-degradation17234
120412nitrate-respiration17632

antibiotic resistance

  • @ref: 120412
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120412
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12041215688acetoin-
12041217234glucose-

enzymes

@refvalueactivityec
120412oxidase+
120412beta-galactosidase+3.2.1.23
120412alcohol dehydrogenase-1.1.1.1
120412gelatinase-
120412amylase-
120412caseinase-3.4.21.50
120412catalase+1.11.1.6
120412tween esterase-
120412gamma-glutamyltransferase+2.3.2.2
120412lecithinase-
120412lipase-
120412lysine decarboxylase-4.1.1.18
120412ornithine decarboxylase-4.1.1.17
120412phenylalanine ammonia-lyase+4.3.1.24
120412protease-
120412tryptophan deaminase-
120412urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120412-+++-++++-+----+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120412-------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountry
2348brackish waterBaltic Sea
67770Brackish waterBaltic Sea
120412Environment, Brackish waterBaltic Sea

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Brackish

taxonmaps

  • @ref: 69479
  • File name: preview.99_1091.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_635;97_738;98_857;99_1091&stattab=map
  • Last taxonomy: Ahrensia kielensis subclade
  • 16S sequence: D88524
  • Sequence Identity:
  • Total samples: 943
  • soil counts: 102
  • aquatic counts: 758
  • animal counts: 73
  • plant counts: 10

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
23481Risk group (German classification)
1204121Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Ahrensia kielensis gene for 16S rRNA, partial sequence, strain: NBRC 15762AB6809601411nuccore76980
2348Agrobacterium kieliense gene for 16S rRNA, partial sequenceD885241407nuccore76980

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ahrensia kielensis DSM 58901120961.3wgspatric1120961
66792Ahrensia kielensis DSM 58902517572121draftimg1120961
67770Ahrensia kielensis DSM 5890GCA_000374465scaffoldncbi1120961

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingno99.478no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no94.397no
69480spore-formingspore-formingAbility to form endo- or exosporesno92.849no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes82.946no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.097yes
69480flagellatedmotile2+Ability to perform flagellated movementyes67.527no

External links

@ref: 2348

culture collection no.: DSM 5890, ATCC 25656, IAM 12618, JCM 20689, CIP 106964, IFO 15762, LMG 133, NBRC 15762, NCIMB 2205

straininfo link

  • @ref: 82832
  • straininfo: 413

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12501429Reclassification of marine Agrobacterium species: Proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev.Uchino Y, Hirata A, Yokota A, Sugiyama JJ Gen Appl Microbiol10.2323/jgam.44.2011998
Phylogeny22021581Pseudahrensia aquimaris gen. nov., sp. nov., isolated from seawater.Jung YT, Park S, Lee JS, Oh TK, Yoon JHInt J Syst Evol Microbiol10.1099/ijs.0.034793-02011Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phyllobacteriaceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/analysisGenetics
Phylogeny26620276Ahrensia marina sp. nov., a dimethylsulfoniopropionate-cleaving bacterium isolated from seawater, and emended descriptions of the genus Ahrensia and Ahrensia kielensis.Liu J, Wang Y, Liu Y, Zhang XHInt J Syst Evol Microbiol10.1099/ijsem.0.0008052015

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2348Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5890)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5890
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39626Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19152
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82832Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID413.1StrainInfo: A central database for resolving microbial strain identifiers
120412Curators of the CIPCollection of Institut Pasteur (CIP 106964)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106964