Strain identifier

BacDive ID: 136227

Type strain: No

Species: Lactobacillus amylovorus

Strain Designation: 2HP 82

Strain history: CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France, Lactobacillus acidophilus, group A3 <- J.L. Johnson, Blacksburg, USA: strain VPI 1754 <- W.E.C. Moore, Blacksburg, USA: strain 2HP 82

NCBI tax ID(s): 1604 (species)

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General

@ref: 35575

BacDive-ID: 136227

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lactobacillus amylovorus 2HP 82 is a facultative anaerobe, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 1604
  • Matching level: species

strain history

@refhistory
67770ATCC 33198 <-- VPI 1754.
35575CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France, Lactobacillus acidophilus, group A3 <- J.L. Johnson, Blacksburg, USA: strain VPI 1754 <- W.E.C. Moore, Blacksburg, USA: strain 2HP 82

doi: 10.13145/bacdive136227.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus amylovorus
  • full scientific name: Lactobacillus amylovorus Nakamura 1981

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus sobrius

@ref: 35575

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus amylovorus

strain designation: 2HP 82

type strain: no

Morphology

cell morphology

  • @ref: 35575
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35575MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
35575CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
35575positivegrowth37mesophilic
67770positivegrowth37mesophilic
35575positivegrowth37-45
35575nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 35575
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
35575nitrate-reduction17632
35575nitrite-reduction16301
35575nitrate+respiration17632

antibiotic resistance

  • @ref: 35575
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite tests

  • @ref: 35575
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
35575oxidase-
35575alcohol dehydrogenase-1.1.1.1
35575catalase-1.11.1.6
35575lysine decarboxylase-4.1.1.18
35575ornithine decarboxylase-4.1.1.17

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35575---------++++----+/----+--+-++++/-+---++--+----------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
35575Blacksburg, VAUSAUSANorth America
67770Hog small intestine
35575Blacksburg, VirginiaUnited States of AmericaUSANorth AmericaAnimal, Hog, small intestinal contents1964

Safety information

risk assessment

  • @ref: 35575
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

GC content

  • @ref: 67770
  • GC-content: 37.3
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 35575

culture collection no.: CIP 103609, ATCC 33198, JCM 2010, CCM 4382, NCFB 2745, VPI 1754

straininfo link

  • @ref: 93435
  • straininfo: 13693

literature

  • topic: Metabolism
  • Pubmed-ID: 10427410
  • title: Heterofermentative metabolism of glucose and ribose and utilisation of citrate by the smooth biotype of Lactobacillus amylovorus NCFB 2745.
  • authors: Whitley K, Marshall VM
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1023/a:1001739532336
  • year: 1999
  • mesh: Citrates/*metabolism, Culture Media, Fermentation, Glucose/*metabolism, Lactobacillus/*classification/growth & development/*metabolism, Ribose/*metabolism
  • topic2: Cultivation

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35575Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103609Collection of Institut Pasteur (CIP 103609)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
93435Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID13693.1