Strain identifier

BacDive ID: 136006

Type strain: No

Species: Priestia megaterium

Strain Designation: 4R6259, Cornell 90

Strain history: CIP <- 1984, ATCC <- Merck: strain 4R6259 (strain Cornell 90)

NCBI tax ID(s): 412384 (species)

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General

@ref: 35303

BacDive-ID: 136006

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Priestia megaterium 4R6259 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 412384
  • Matching level: species

strain history

  • @ref: 35303
  • history: CIP <- 1984, ATCC <- Merck: strain 4R6259 (strain Cornell 90)

doi: 10.13145/bacdive136006.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Priestia
  • species: Priestia megaterium
  • full scientific name: Priestia megaterium (de Bary 1884) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus megaterium

@ref: 35303

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus megaterium

strain designation: 4R6259, Cornell 90

type strain: no

Morphology

cell morphology

  • @ref: 35303
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 35303

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35303MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
35303CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
35303positivegrowth30mesophilic
35303positivegrowth22-45
35303nogrowth10psychrophilic
35303nogrowth55thermophilic

culture pH

  • @ref: 35303
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 35303
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 35303
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
35303NaClpositivegrowth0-8 %
35303NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
35303citrate-carbon source16947
35303esculin+hydrolysis4853
35303hippurate-hydrolysis606565
35303nitrate-reduction17632
35303nitrite+reduction16301
35303nitrate-respiration17632

metabolite production

  • @ref: 35303
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 35303
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
35303oxidase+
35303beta-galactosidase+3.2.1.23
35303alcohol dehydrogenase-1.1.1.1
35303gelatinase+
35303amylase+
35303DNase-
35303caseinase+3.4.21.50
35303catalase+1.11.1.6
35303tween esterase-
35303gamma-glutamyltransferase+2.3.2.2
35303lecithinase-
35303lipase-
35303lysine decarboxylase-4.1.1.18
35303ornithine decarboxylase-4.1.1.17
35303protease+
35303urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35303--++------++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35303+--+++---+++----++---+-+++-+-+++--+++-++------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
35303++++--+++++++++++++++++--++-+--+++++-----+-----+-+++-++-+-+++---+--------+-+--+++-+---+-++++++++---

Safety information

risk assessment

  • @ref: 35303
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35303

culture collection no.: CIP 101048, ATCC 9885, CCM 1462, CECT 370, CCTM 265, LMG 11162, NCIMB 8291

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity4977688Effect of lysozyme on resting spores of Bacillus megaterium.Suzuki Y, Rode LJJ Bacteriol10.1128/jb.98.1.238-245.19691969Alanine/pharmacology, Alkanes/pharmacology, Bacillus/drug effects, Bacillus megaterium/*drug effects, Bacteriolysis, Enzymes/pharmacology, Glucose/pharmacology, Leucine/pharmacology, Microscopy, Electron, Muramidase/*pharmacology, Nucleosides/pharmacology, Spores/*drug effects/growth & developmentEnzymology
Pathogenicity15859069Calf antibiotic and sulfonamide test (CAST) for screening antibiotic and sulfonamide residues in calf carcasses.Dey BP, Reamer RP, Thaker NH, Thaler AMJ AOAC Int2005Animals, Anti-Bacterial Agents/*analysis, Anti-Infective Agents/*analysis, Bacillus megaterium/drug effects, Bacillus subtilis/drug effects, Biological Assay, Cattle, Culture Media, Drug Residues/*analysis, Kidney/chemistry, Liver/chemistry, Meat/*analysis, Muscle, Skeletal/chemistry, Reference Standards, Sulfonamides/*analysis, TemperatureCultivation
Pathogenicity27131738Novel bioassay using Bacillus megaterium to detect tetracycline in milk.Tumini M, Nagel OG, Molina P, Althaus RLRev Argent Microbiol10.1016/j.ram.2016.02.0012016Animals, Anti-Bacterial Agents/*analysis/pharmacology, Argentina, Bacillus megaterium/*drug effects/physiology, Biological Assay/*methods, Chloramphenicol/analysis/pharmacology, Drug Residues/*analysis/pharmacology, Food Contamination/*analysis/legislation & jurisprudence, Maximum Allowable Concentration, Milk/*chemistry, Sensitivity and Specificity, Spores, Bacterial/drug effects, Tetracyclines/*analysis/pharmacology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35303Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101048Collection of Institut Pasteur (CIP 101048)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym