Strain identifier

BacDive ID: 1360

Type strain: Yes

Species: Lentibacillus salarius

Strain Designation: BH139, 4.101

Strain history: CIP <- 2005, KCTC <- C.H. Kim <- D.J. Park: strain BH139

NCBI tax ID(s): 284578 (species)

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General

@ref: 6450

BacDive-ID: 1360

DSM-Number: 16459

keywords: 16S sequence, Bacteria, obligate aerobe, moderately halophilic, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Lentibacillus salarius BH139 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from saline soil.

NCBI tax id

  • NCBI tax id: 284578
  • Matching level: species

strain history

@refhistory
6450<- C.-J. Kim <- D.-J. Park; BH139
67771<- CJ Kim, KRIBB
122359CIP <- 2005, KCTC <- C.H. Kim <- D.J. Park: strain BH139

doi: 10.13145/bacdive1360.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus salarius
  • full scientific name: Lentibacillus salarius Jeon et al. 2005

@ref: 6450

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus salarius

full scientific name: Lentibacillus salarius Jeon et al. 2005

strain designation: BH139, 4.101

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
22997positive1.5-3.0 µm0.2-0.3 µmrod-shapedyes
67771rod-shapedyes
67771positive
122359positiverod-shapedyes

colony morphology

@refcolony colorcolony shapemedium used
22997creamcircularMA supplemented with 10 % (w/v) NaCl
122359

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6450BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 80.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l MnSO4 0.01 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
22997MA supplemented with 10 % (w/v) NaClyes
42173Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
122359CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6450positivegrowth30mesophilic
22997positivegrowth15.0-50.0
22997positiveoptimum30.0-35.0mesophilic
42173positivegrowth30mesophilic
67771positivegrowth34mesophilic
122359positivegrowth10-37
122359nogrowth45thermophilic
122359nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
22997positivegrowth6.0-8.5alkaliphile
22997positiveoptimum7.0-7.5
122359nogrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22997obligate aerobe
67771aerobe

spore formation

  • @ref: 22997
  • spore description: spherical or oval,terminal,in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
22997moderately halophilicNaClpositivegrowth1.0-20.0 %(w/v)
22997NaCloptimum12.0-14.0 %
22997NaClnogrowth>20.0 %(w/v)
122359NaClnogrowth0 %
122359NaClnogrowth2 %
122359NaClnogrowth4 %
122359NaClnogrowth6 %
122359NaClnogrowth8 %
122359NaClnogrowth10 %

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2299715963ribitol-builds acid from
2299718305arbutin-builds acid from
2299728053melibiose-builds acid from
2299716634raffinose-builds acid from
2299726546rhamnose-builds acid from
2299717814salicin-builds acid from
22997casein-hydrolysis
2299717368hypoxanthine-hydrolysis
2299717895L-tyrosine-hydrolysis
2299728017starch-hydrolysis
2299753426tween 80-hydrolysis
2299716199urea-hydrolysis
2299715318xanthine-hydrolysis
2299736219alpha-lactose+builds acid from
2299715824D-fructose+builds acid from
2299717634D-glucose+builds acid from
2299716899D-mannitol+builds acid from
2299716024D-mannose+builds acid from
2299716988D-ribose+builds acid from
2299716551D-trehalose+builds acid from
2299765327D-xylose+builds acid from
2299717754glycerol+builds acid from
2299730849L-arabinose+builds acid from
2299717306maltose+builds acid from
229974853esculin+hydrolysis
2299717632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
122359606565hippurate+hydrolysis
12235917632nitrate+reduction
12235916301nitrite-reduction
12235917632nitrate-respiration

metabolite production

  • @ref: 122359
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12235915688acetoin-
12235917234glucose-

enzymes

@refvalueactivityec
22997catalase+1.11.1.6
22997cytochrome oxidase-1.9.3.1
22997urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122359oxidase-
122359beta-galactosidase-3.2.1.23
122359alcohol dehydrogenase-1.1.1.1
122359gelatinase+
122359amylase-
122359caseinase-3.4.21.50
122359catalase+1.11.1.6
122359tween esterase+
122359gamma-glutamyltransferase+2.3.2.2
122359lecithinase-
122359lipase+
122359lysine decarboxylase+4.1.1.18
122359ornithine decarboxylase+4.1.1.17
122359protease-
122359tryptophan deaminase-
122359urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122359-+++-+----++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122359-------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
22997saline soilXin-Jiang ProvinceChinaCHNAsiamarine agar 2216 (MA;Difco), to which 15 % (w/v) NaCl had been added (final NaCl concentration: 16.94 %, w/v)3 days28.0
6450salt lake soilChinaCHNAsia
67771From soil sediment of a salt lakeXinjiang ProvinceChinaCHNAsia
122359Environment, Soil, salt lakeXinjiangChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_72705.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3088;97_11300;98_13897;99_72705&stattab=map
  • Last taxonomy: Lentibacillus salarius
  • 16S sequence: AY667493
  • Sequence Identity:
  • Total samples: 122
  • soil counts: 14
  • aquatic counts: 26
  • animal counts: 71
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64501Risk group (German classification)
1223591Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6450
  • description: Lentibacillus salarius strain BH139 16S ribosomal RNA gene, partial sequence
  • accession: AY667493
  • length: 1404
  • database: ena
  • NCBI tax ID: 284578

GC content

@refGC-contentmethod
645043
2299743high performance liquid chromatography (HPLC)
6777143.0high performance liquid chromatography (HPLC)

External links

@ref: 6450

culture collection no.: DSM 16459, KCTC 3911, CIP 108797

straininfo link

  • @ref: 71011
  • straininfo: 138987

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15879279Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus.Jeon CO, Lim JM, Lee JC, Lee GS, Lee JM, Xu LH, Jiang CL, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.63462-02005Bacillaceae/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Flagella/physiology, Genes, Bacterial, Genes, rRNA, Gentian Violet, Geologic Sediments/*microbiology, Growth Inhibitors/pharmacology, Molecular Sequence Data, Movement, Phenazines, Phylogeny, Quinones/analysis/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/pharmacology, *Soil Microbiology, Spores, Bacterial, TemperatureEnzymology
Phylogeny17267980Lentibacillus kapialis sp. nov., from fermented shrimp paste in Thailand.Pakdeeto A, Tanasupawat S, Thawai C, Moonmangmee S, Kudo T, Itoh TInt J Syst Evol Microbiol10.1099/ijs.0.64315-02007Animals, Bacillaceae/*classification/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Decapoda/*microbiology, Diaminopimelic Acid/analysis, Fatty Acids/analysis/chemistry, Fermentation, *Food Microbiology, Genes, rRNA, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids/analysis/chemistry, Phylogeny, Pigments, Biological/biosynthesis, Quinones/analysis/chemistry, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology
Phylogeny17329773Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China.Yuan S, Ren P, Liu J, Xue Y, Ma Y, Zhou PInt J Syst Evol Microbiol10.1099/ijs.0.64547-02007Bacillaceae/*classification/genetics/isolation & purification/physiology, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, SaltsGenetics
Phylogeny18175680Lentibacillus salinarum sp. nov., isolated from a marine solar saltern in Korea.Lee SY, Choi WY, Oh TK, Yoon JHInt J Syst Evol Microbiol10.1099/ijs.0.65351-02008Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Genes, rRNA, Geologic Sediments/*microbiology, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, *Sodium Chloride, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6450Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16459)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16459
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22997Che Ok Jeon,Jee-Min Lim,Jae-Chan Lee,Gye Suk Lee,Jung-Min Lee,Li-Hua Xu,Cheng-Lin Jiang,Chang-Jin Kim10.1099/ijs.0.63462-0Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus LentibacillusIJSEM 55: 1339-1343 200515879279
42173Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6466
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71011Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID138987.1StrainInfo: A central database for resolving microbial strain identifiers
122359Curators of the CIPCollection of Institut Pasteur (CIP 108797)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108797