Strain identifier

BacDive ID: 135983

Type strain: No

Species: Pseudomonas putida

Strain history: CIP <- 1952, R. Stanier, Inst. Pasteur, Paris, France: strain PF 20, Pseudomonas fluorescens <- F. Jacob

NCBI tax ID(s): 303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 35276

BacDive-ID: 135983

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas putida CIP 52.195 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 35276
  • history: CIP <- 1952, R. Stanier, Inst. Pasteur, Paris, France: strain PF 20, Pseudomonas fluorescens <- F. Jacob

doi: 10.13145/bacdive135983.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 35276

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

type strain: no

Morphology

cell morphology

  • @ref: 35276
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 35276
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35276MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
35276CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
35276CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
35276positivegrowth30mesophilic
35276positivegrowth5-30
35276nogrowth37mesophilic
35276nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 35276
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
35276NaClpositivegrowth0-6 %
35276NaClnogrowth8 %
35276NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3527616947citrate-carbon source
352764853esculin-hydrolysis
3527617632nitrate-reduction
3527616301nitrite-reduction
3527617632nitrate-respiration

antibiotic resistance

  • @ref: 35276
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 35276
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
35276oxidase+
35276beta-galactosidase-3.2.1.23
35276alcohol dehydrogenase+1.1.1.1
35276gelatinase-
35276amylase-
35276DNase-
35276caseinase-3.4.21.50
35276catalase+1.11.1.6
35276tween esterase-
35276lecithinase-
35276lipase+
35276lysine decarboxylase-4.1.1.18
35276ornithine decarboxylase-4.1.1.17
35276protease-
35276tryptophan deaminase-
35276urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35276-+++-++-++++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
35276-+-----------------+-----------------------+----+-++--+-+---+---+--+-----++------++-+--++--++--+---

Safety information

risk assessment

  • @ref: 35276
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35276

culture collection no.: CIP 52.195

straininfo link

  • @ref: 93239
  • straininfo: 69790

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35276Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.195Collection of Institut Pasteur (CIP 52.195)
68382Automatically annotated from API zym
93239Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID69790.1