Strain identifier

BacDive ID: 135938

Type strain: No

Species: Clostridium perfringens

Strain Designation: 150

Strain history: CIP <- 2001, M.R. Popoff, Inst. Pasteur, Paris, France: strain 150

NCBI tax ID(s): 1502 (species)

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General

@ref: 35217

BacDive-ID: 135938

keywords: Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Clostridium perfringens 150 is an anaerobe, spore-forming, mesophilic bacterium of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

@refhistory
352172001, M.R. Popoff, Inst. Pasteur, Paris, France: strain 150
35217CIP <- 2001, M.R. Popoff, Inst. Pasteur, Paris, France: strain 150

doi: 10.13145/bacdive135938.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 35217

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

strain designation: 150

type strain: no

Morphology

cell morphology

  • @ref: 35217
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 35217
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35217MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
35217CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 35217
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 35217
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 35217
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
352174853esculin-hydrolysis
3521717632nitrate-reduction
3521716301nitrite+reduction
3521717632nitrate+respiration

metabolite production

  • @ref: 35217
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
35217oxidase-
35217beta-galactosidase+3.2.1.23
35217gelatinase+/-
35217amylase+
35217DNase+
35217caseinase+3.4.21.50
35217catalase-1.11.1.6
35217tween esterase+
35217lecithinase+
35217lipase-
35217protease-
35217urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35217-+++----+-++++++-+--

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
35217NormandyFranceFRAEurope
35217NormandyFranceFRAEuropeAnimal, Horse1996

Safety information

risk assessment

  • @ref: 35217
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35217

culture collection no.: CIP 106894

straininfo link

  • @ref: 93200
  • straininfo: 67923

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35217Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106894Collection of Institut Pasteur (CIP 106894)
68382Automatically annotated from API zym
93200Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67923.1