Strain identifier

BacDive ID: 135932

Type strain: No

Species: Streptococcus pneumoniae

Strain Designation: SSISP 25 F/2

Strain history: CIP <- 2000, A. Ostergard, Stat. Ser. Inst., Copenhagen, Denmark: strain SSISP 25 F/2

NCBI tax ID(s): 1313 (species)

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General

@ref: 35209

BacDive-ID: 135932

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, ovoid-shaped

description: Streptococcus pneumoniae SSISP 25 F/2 is a facultative anaerobe, mesophilic, Gram-positive bacterium of the family Streptococcaceae.

NCBI tax id

  • NCBI tax id: 1313
  • Matching level: species

strain history

@refhistory
352092000, A. Ostergard, Stat. Ser. Inst., Copenhagen, Denmark: strain SSISP 25 F/2
35209CIP <- 2000, A. Ostergard, Stat. Ser. Inst., Copenhagen, Denmark: strain SSISP 25 F/2

doi: 10.13145/bacdive135932.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus pneumoniae
  • full scientific name: Streptococcus pneumoniae (Klein 1884) Chester 1901 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Micrococcus pneumoniae
    20215Staphylococcus pneumoniae

@ref: 35209

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus pneumoniae

strain designation: SSISP 25 F/2

type strain: no

Morphology

cell morphology

  • @ref: 35209
  • gram stain: positive
  • cell shape: ovoid-shaped
  • motility: no

colony morphology

  • @ref: 35209
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35209MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
35209CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperature
35209positivegrowth37
35209positivegrowth25-41
35209nogrowth10
35209nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 35209
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 35209
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
352094853esculin-hydrolysis
3520917632nitrate-reduction
3520916301nitrite-reduction
68371Potassium 5-ketogluconate+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837117151xylitol-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

antibiotic resistance

@refmetaboliteis antibioticis sensitiveis resistantChEBI
352090129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesyesno
35209optochinyesyesno86455

metabolite tests

  • @ref: 35209
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin+3.4.21.1
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
35209oxidase-
35209beta-galactosidase+3.2.1.23
35209alcohol dehydrogenase-1.1.1.1
35209catalase-1.11.1.6
35209gamma-glutamyltransferase+2.3.2.2
35209lysine decarboxylase-4.1.1.18
35209ornithine decarboxylase-4.1.1.17
68382alpha-fucosidase-3.2.1.51
68382beta-glucosidase-3.2.1.21
68382trypsin-3.4.21.4

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35209-----++--+-+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35209---------+/-+/-+/-+/-------------+/-+/-+/-+/-+/-----+/-+/-+/--+/-+/---------+

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinent
35209USAUSANorth America
35209United States of AmericaUSANorth America

Safety information

risk assessment

  • @ref: 35209
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35209

culture collection no.: CIP 106590

straininfo link

  • @ref: 93196
  • straininfo: 70386

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35209Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106590Collection of Institut Pasteur (CIP 106590)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
93196Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID70386.1