Strain identifier

BacDive ID: 135760

Type strain: No

Species: Clostridioides difficile

Strain history: CIP <- 2011, P. Bouvet, Inst. Pasteur, Paris, France: strain AIP 2009215, Clostridium difficile

NCBI tax ID(s): 1496 (species)

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General

@ref: 34993

BacDive-ID: 135760

keywords: Bacteria, mesophilic, Gram-positive, rod-shaped

description: Clostridioides difficile CIP 110322 is a mesophilic, Gram-positive, rod-shaped bacterium of the family Peptostreptococcaceae.

NCBI tax id

  • NCBI tax id: 1496
  • Matching level: species

strain history

@refhistory
349932011, P. Bouvet, Inst. Pasteur, Paris, France: strain AIP 2009215
34993CIP <- 2011, P. Bouvet, Inst. Pasteur, Paris, France: strain AIP 2009215, Clostridium difficile

doi: 10.13145/bacdive135760.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptostreptococcaceae
  • genus: Clostridioides
  • species: Clostridioides difficile
  • full scientific name: Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016

  • synonyms

    @refsynonym
    20215Bacillus difficilis
    20215Peptoclostridium difficile
    20215Clostridium difficile

@ref: 34993

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Peptostreptococcaceae

genus: Clostridioides

species: Clostridioides difficile

type strain: no

Morphology

cell morphology

  • @ref: 34993
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34993MEDIUM 224 - for anaerobic bacteria TGVyesDistilled water make up to (1000.000 ml);Glucose (10.000 g);Yeast extract (20.000 g);Resazurin (2.000 mg);Trypto casein soy broth (30.000 g);L-Cysteine (0.500 g);Hemin solution - M00149 (25.000 ml);Vitamins solution - M00850 (10.000 ml)
34993CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 34993
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3499317632nitrate-reduction
3499316301nitrite-reduction

metabolite production

  • @ref: 34993
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
34993urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34993-----------+--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
34993Seine Saint DenisFranceFRAEurope
34993Seine Saint DenisFranceFRAEuropeHuman, Faeces2009

Safety information

risk assessment

  • @ref: 34993
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 34993

culture collection no.: CIP 110322

straininfo link

  • @ref: 93049
  • straininfo: 395489

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34993Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110322Collection of Institut Pasteur (CIP 110322)
68382Automatically annotated from API zym
93049Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID395489.1