Strain identifier

BacDive ID: 135753

Type strain: No

Species: Pseudomonas putida

Strain history: CIP <- 2005, O. Bouvet, Inserm, Paris, France <- P. Grimont, Inst. Pasteur, Paris, France <- LMG

NCBI tax ID(s): 303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 34978

BacDive-ID: 135753

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas putida CIP 108916 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 34978
  • history: CIP <- 2005, O. Bouvet, Inserm, Paris, France <- P. Grimont, Inst. Pasteur, Paris, France <- LMG

doi: 10.13145/bacdive135753.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 34978

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

type strain: no

Morphology

cell morphology

  • @ref: 34978
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 34978
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34978MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
34978CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
34978positivegrowth30mesophilic
34978positivegrowth5-30
34978nogrowth37mesophilic
34978nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 34978
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
34978NaClpositivegrowth0-8 %
34978NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3497816947citrate+carbon source
349784853esculin+hydrolysis
3497817632nitrate+reduction
3497816301nitrite-reduction
3497817632nitrate-respiration

metabolite production

  • @ref: 34978
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
34978oxidase+
34978beta-galactosidase-3.2.1.23
34978alcohol dehydrogenase+1.1.1.1
34978gelatinase+/-
34978amylase-
34978DNase+
34978caseinase-3.4.21.50
34978catalase+1.11.1.6
34978tween esterase-
34978lecithinase-
34978lipase-
34978lysine decarboxylase-4.1.1.18
34978protease-
34978tryptophan deaminase-
34978urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34978-+++-++-+-++----++++

Safety information

risk assessment

  • @ref: 34978
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 34978

culture collection no.: CIP 108916, LMG 1246, ATCC 17430, NCIMB 10534, NRRL B-3

straininfo link

  • @ref: 93043
  • straininfo: 4214

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34978Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108916Collection of Institut Pasteur (CIP 108916)
68382Automatically annotated from API zym
93043Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID4214.1