Strain identifier

BacDive ID: 13570

Type strain: Yes

Species: Sinorhizobium americanum

Strain Designation: MexA1

Strain history: CIP <- 2004, E. Martinez-Romero, Centr. Investig. Fijacion Nitrogeno, Mexico Univ., Morelos, Mexico: strain CFNEI 156

NCBI tax ID(s): 194963 (species)

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General

@ref: 5662

BacDive-ID: 13570

DSM-Number: 15007

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Sinorhizobium americanum MexA1 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from nitrogen fixing nodules of Acacia plants.

NCBI tax id

  • NCBI tax id: 194963
  • Matching level: species

strain history

@refhistory
5662<- E. Martínez-Romero; CFNEI 156 <- I. Toledo
116327CIP <- 2004, E. Martinez-Romero, Centr. Investig. Fijacion Nitrogeno, Mexico Univ., Morelos, Mexico: strain CFNEI 156

doi: 10.13145/bacdive13570.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Rhizobiaceae
  • genus: Sinorhizobium
  • species: Sinorhizobium americanum
  • full scientific name: Sinorhizobium americanum corrig. Toledo et al. 2004

  • synonyms

    @refsynonym
    20215Sinorhizobium americanus
    20215Ensifer americanum
    20215Ensifer americanus

@ref: 5662

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Rhizobiaceae

genus: Ensifer

species: Ensifer americanus

full scientific name: Ensifer americanus (Toledo et al. 2004) Wang et al. 2015

strain designation: MexA1

type strain: yes

Morphology

cell morphology

  • @ref: 116327
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5662RHIZOBIUM MEDIUM (DSMZ Medium 98)yeshttps://mediadive.dsmz.de/medium/98Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
33237MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116327CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
5662positivegrowth28-30mesophilic
33237positivegrowth30mesophilic
116327positivegrowth25-37mesophilic
116327nogrowth10psychrophilic
116327nogrowth41thermophilic
116327nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
116327NaClpositivegrowth0 %
116327NaClnogrowth2 %
116327NaClnogrowth4 %
116327NaClnogrowth6 %
116327NaClnogrowth8 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11632716947citrate-carbon source
1163274853esculin-hydrolysis
116327606565hippurate-hydrolysis
11632717632nitrate+reduction
11632716301nitrite-reduction

antibiotic resistance

  • @ref: 116327
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116327
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11632715688acetoin-
11632717234glucose-

enzymes

@refvalueactivityec
116327oxidase-
116327beta-galactosidase+3.2.1.23
116327gelatinase-
116327catalase+1.11.1.6
116327gamma-glutamyltransferase+2.3.2.2
116327phenylalanine ammonia-lyase+4.3.1.24
116327urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116327-+++-+--+-++-+-++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116327++-+--++--+++++----+---++-+----+++-++------------+------------+-------++-+-+---++-------++++-+-+---

Isolation, sampling and environmental information

isolation

  • @ref: 5662
  • sample type: nitrogen fixing nodules of Acacia plants
  • host species: Acacia
  • geographic location: Morelos, Sierra de Huautla
  • country: Mexico
  • origin.country: MEX
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_273.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_84;97_88;98_232;99_273&stattab=map
  • Last taxonomy: Rhizobiaceae
  • 16S sequence: AF506513
  • Sequence Identity:
  • Total samples: 9487
  • soil counts: 5353
  • aquatic counts: 1562
  • animal counts: 1094
  • plant counts: 1478

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56621Risk group (German classification)
1163271Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5662
  • description: Sinorhizobium americanum strain CFNEI 156 16S ribosomal RNA gene, partial sequence
  • accession: AF506513
  • length: 1437
  • database: ena
  • NCBI tax ID: 194963

External links

@ref: 5662

culture collection no.: DSM 15007, ATCC BAA 532, CIP 108390, CFNEI 156

straininfo link

  • @ref: 82757
  • straininfo: 134445

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22633818Sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (Phaseolus vulgaris L.) in a Northern Tunisian field.Mnasri B, Saidi S, Chihaoui SA, Mhamdi RSyst Appl Microbiol10.1016/j.syapm.2012.04.0032012Bacterial Proteins/genetics, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Nitrogen Fixation, Phaseolus/*microbiology, Phylogeny, *Plant Root Nodulation, Plant Roots/*microbiology, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Sinorhizobium/classification/genetics/*isolation & purification/*physiology, *Symbiosis, TunisiaTranscriptome
31151153Exo-Metabolites of Phaseolus vulgaris-Nodulating Rhizobial Strains.Montes-Grajales D, Esturau-Escofet N, Esquivel B, Martinez-Romero EMetabolites10.3390/metabo90601052019

Reference

@idauthorscataloguedoi/urltitle
5662Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15007)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15007
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33237Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6007
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82757Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID134445.1StrainInfo: A central database for resolving microbial strain identifiers
116327Curators of the CIPCollection of Institut Pasteur (CIP 108390)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108390