Strain identifier

BacDive ID: 135592

Type strain: No

Species: Bordetella pertussis

Strain Designation: 28 H.Am

Strain history: CIP <- 1949, R. Legroux, Inst. Pasteur, Paris, France: strain 28 H.Am <- USA

NCBI tax ID(s): 520 (species)

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General

@ref: 34765

BacDive-ID: 135592

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Bordetella pertussis 28 H.Am is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Alcaligenaceae.

NCBI tax id

  • NCBI tax id: 520
  • Matching level: species

strain history

  • @ref: 34765
  • history: CIP <- 1949, R. Legroux, Inst. Pasteur, Paris, France: strain 28 H.Am <- USA

doi: 10.13145/bacdive135592.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Bordetella
  • species: Bordetella pertussis
  • full scientific name: Bordetella pertussis (Bergey et al. 1923) Moreno-López 1952 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Hemophilus pertussis

@ref: 34765

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Bordetella

species: Bordetella pertussis

strain designation: 28 H.Am

type strain: no

Morphology

cell morphology

  • @ref: 34765
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 34765

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34765MEDIUM 21 - for BordetellayesDistilled water make up to (1000.000 ml);Horse blood (250.000 ml);Bordetgengou agar (30.000 g)
34765CIP Medium 21yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=21

culture temp

  • @ref: 34765
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 34765
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3476517632nitrate-reduction
3476516301nitrite-reduction

metabolite production

  • @ref: 34765
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
34765oxidase+
34765catalase+1.11.1.6
34765urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34765-+++-+----+---+-----

Safety information

risk assessment

  • @ref: 34765
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 34765

culture collection no.: CIP A103

straininfo link

  • @ref: 92918
  • straininfo: 105290

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34765Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20A103Collection of Institut Pasteur (CIP A103)
68382Automatically annotated from API zym
92918Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID105290.1