Strain identifier

BacDive ID: 135293

Type strain: No

Species: Lactobacillus acidophilus

Strain Designation: R-1-1

Strain history: CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France: strain VPI 0328, Lactobacillus acidophilus, group A1 <- ATCC <- W.L. Kulp: strain R-1-1

NCBI tax ID(s): 1579 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9.1:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 9.3 (current version)

General

@ref: 34370

BacDive-ID: 135293

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative

description: Lactobacillus acidophilus R-1-1 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 1579
  • Matching level: species

strain history

@refhistory
67770T. Mitsuoka S1-64 <-- ISL <-- ATCC 4355 <-- W. L. Kulp R-1-1.
34370CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France: strain VPI 0328, Lactobacillus acidophilus, group A1 <- ATCC <- W.L. Kulp: strain R-1-1

doi: 10.13145/bacdive135293.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus acidophilus
  • full scientific name: Lactobacillus acidophilus (Moro 1900) Hansen and Mocquot 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus acidophilus

@ref: 34370

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus acidophilus

strain designation: R-1-1

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
125438negative99.5
34370positiverod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34370MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
34370CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
34370positivegrowth37
67770positivegrowth37
34370positivegrowth30-45
34370nogrowth5
34370nogrowth10
34370nogrowth15

Physiology and metabolism

oxygen tolerance

  • @ref: 34370
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3437017632nitrate-reduction
3437016301nitrite-reduction
3437017632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837112936D-galactose+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite tests

  • @ref: 34370
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
34370oxidase-
34370alcohol dehydrogenase-1.1.1.1
34370catalase-1.11.1.6
34370lysine decarboxylase-4.1.1.18
34370ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34370--+--+++--++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
34370---------++++--------++++++++-++--+---+----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
34370---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample type
34370USAUSANorth America
67770Rat intestine
34370United States of AmericaUSANorth AmericaAnimal, Rat

Safety information

risk assessment

  • @ref: 34370
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 124043
  • description: Lactobacillus acidophilus gene for 16S rRNA, partial sequence, strain: JCM 1021.
  • accession: AB289011
  • length: 681
  • database: nuccore
  • NCBI tax ID: 1579

GC content

  • @ref: 67770
  • GC-content: 33
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno99.5no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.68no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no59.104yes
125438spore-formingspore-formingAbility to form endo- or exosporesno89.845no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno98.5yes
125438motile2+flagellatedAbility to perform flagellated movementno78.338no

External links

@ref: 34370

culture collection no.: CIP 103596, ATCC 4355, JCM 1021, KCTC 3140, LMG 11469, VPI 0328

straininfo link

  • @ref: 92670
  • straininfo: 3198

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Phylogeny9178566Isolation and partial amino acid sequence of bacteriocins produced by Lactobacillus acidophilus.Tahara T, Kanatani KBiosci Biotechnol Biochem1997Amino Acid Sequence, Bacteriocins/chemistry/*isolation & purification, Lactobacillus/*drug effects/growth & development, Lactobacillus acidophilus/*chemistry, Molecular Sequence Data, Molecular Weight, Species SpecificityGenetics
Phylogeny28862569Isolation and Partial Amino Add Sequence of Bacteriocins Produced by Lactobacillus acidophilus.Tahara T, Kanatani KBiosci Biotechnol Biochem199710.1271/bbb.61.884
30991529Freeze-Drying Injury of Lactobacillus Acidophilus.Bozoglu TF, Gurakan GCJ Food Prot198910.4315/0362-028X-52.4.259

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34370Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103596Collection of Institut Pasteur (CIP 103596)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
92670Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID3198.1
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695