Strain identifier

BacDive ID: 13527

Type strain: Yes

Species: Pyrodictium abyssi

Strain Designation: AV2

Strain history: DSM 6158 <-- K. O. Stetter AV2.

NCBI tax ID(s): 391605 (strain), 54256 (species)

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General

@ref: 2507

BacDive-ID: 13527

DSM-Number: 6158

keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Pyrodictium abyssi AV2 is an anaerobe, hyperthermophilic archaeon that was isolated from black smoker at 2011 m depth.

NCBI tax id

NCBI tax idMatching level
391605strain
54256species

strain history

@refhistory
2507<- K.O. Stetter, AV2
67770DSM 6158 <-- K. O. Stetter AV2.

doi: 10.13145/bacdive13527.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Desulfurococcales
  • family: Pyrodictiaceae
  • genus: Pyrodictium
  • species: Pyrodictium abyssi
  • full scientific name: Pyrodictium abyssi Pley and Stetter 1991

@ref: 2507

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Desulfurococcales

family: Pyrodictiaceae

genus: Pyrodictium

species: Pyrodictium abyssi

full scientific name: Pyrodictium abyssi Pley and Stetter 1991

strain designation: AV2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 2507
  • name: PYRODICTIUM MEDIUM (DSMZ Medium 283)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/283
  • composition: Name: PYRODICTIUM MEDIUM (DSMZ Medium 283; with strain-specific modifications) Composition: Sulfur 29.2683 g/l MgCl2 x 6 H2O 2.68293 g/l Yeast extract 0.487805 g/l Na2S x 9 H2O 0.487805 g/l KH2PO4 0.487805 g/l KCl 0.321951 g/l NaBr 0.0487805 g/l MgSO4 x 7 H2O 0.0292683 g/l Nitrilotriacetic acid 0.0146341 g/l NaCl 0.0097561 g/l (NH4)2SO4 0.0097561 g/l SrCl2 x 6 H2O 0.00682927 g/l MnSO4 x H2O 0.00487805 g/l ZnSO4 x 7 H2O 0.0017561 g/l CoSO4 x 7 H2O 0.0017561 g/l FeSO4 x 7 H2O 0.00097561 g/l CaCl2 x 2 H2O 0.00097561 g/l Sodium resazurin 0.000487805 g/l NiCl2 x 6 H2O 0.000292683 g/l AlK(SO4)2 x 12 H2O 0.000195122 g/l H3BO3 9.7561e-05 g/l CuSO4 x 5 H2O 9.7561e-05 g/l Na2MoO4 x 2 H2O 9.7561e-05 g/l KI 4.87805e-05 g/l Na2WO4 x 2 H2O 3.90244e-06 g/l Na2SeO3 x 5 H2O 2.92683e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
2507positivegrowth98hyperthermophilic
67770positivegrowth95hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2507
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2507black smoker at 2011 m depthGulf of California, Guaymas BasinMexicoMEXNorth America
67770Abyssal active 'black smoker' off GuaymasMexicoMEXNorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 2507
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pyrodictium abyssi DSM 6158 16S ribosomal RNA gene, partial sequenceKC1451531307ena391605
20218Pyrodictium abyssi strain NBRC 100439 16S ribosomal RNA gene, partial sequenceKC1392491317ena54256
2507P.abyssi 16S rRNA geneX995591351ena391605

GC content

@refGC-contentmethod
250759.0
6777059.3thermal denaturation, midpoint method (Tm)
6777059high performance liquid chromatography (HPLC)

External links

@ref: 2507

culture collection no.: DSM 6158, JCM 9394, ATCC 49828, NBRC 100439

straininfo link

  • @ref: 82718
  • straininfo: 42704

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2507Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6158)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6158
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
82718Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42704.1StrainInfo: A central database for resolving microbial strain identifiers