Strain identifier
BacDive ID: 13527
Type strain:
Species: Pyrodictium abyssi
Strain Designation: AV2
Strain history: DSM 6158 <-- K. O. Stetter AV2.
NCBI tax ID(s): 391605 (strain), 54256 (species)
General
@ref: 2507
BacDive-ID: 13527
DSM-Number: 6158
keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Pyrodictium abyssi AV2 is an anaerobe, hyperthermophilic archaeon that was isolated from black smoker at 2011 m depth.
NCBI tax id
NCBI tax id | Matching level |
---|---|
391605 | strain |
54256 | species |
strain history
@ref | history |
---|---|
2507 | <- K.O. Stetter, AV2 |
67770 | DSM 6158 <-- K. O. Stetter AV2. |
doi: 10.13145/bacdive13527.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Desulfurococcales
- family: Pyrodictiaceae
- genus: Pyrodictium
- species: Pyrodictium abyssi
- full scientific name: Pyrodictium abyssi Pley and Stetter 1991
@ref: 2507
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Desulfurococcales
family: Pyrodictiaceae
genus: Pyrodictium
species: Pyrodictium abyssi
full scientific name: Pyrodictium abyssi Pley and Stetter 1991
strain designation: AV2
type strain: yes
Culture and growth conditions
culture medium
- @ref: 2507
- name: PYRODICTIUM MEDIUM (DSMZ Medium 283)
- growth: yes
- link: https://mediadive.dsmz.de/medium/283
- composition: Name: PYRODICTIUM MEDIUM (DSMZ Medium 283; with strain-specific modifications) Composition: Sulfur 29.2683 g/l MgCl2 x 6 H2O 2.68293 g/l Yeast extract 0.487805 g/l Na2S x 9 H2O 0.487805 g/l KH2PO4 0.487805 g/l KCl 0.321951 g/l NaBr 0.0487805 g/l MgSO4 x 7 H2O 0.0292683 g/l Nitrilotriacetic acid 0.0146341 g/l NaCl 0.0097561 g/l (NH4)2SO4 0.0097561 g/l SrCl2 x 6 H2O 0.00682927 g/l MnSO4 x H2O 0.00487805 g/l ZnSO4 x 7 H2O 0.0017561 g/l CoSO4 x 7 H2O 0.0017561 g/l FeSO4 x 7 H2O 0.00097561 g/l CaCl2 x 2 H2O 0.00097561 g/l Sodium resazurin 0.000487805 g/l NiCl2 x 6 H2O 0.000292683 g/l AlK(SO4)2 x 12 H2O 0.000195122 g/l H3BO3 9.7561e-05 g/l CuSO4 x 5 H2O 9.7561e-05 g/l Na2MoO4 x 2 H2O 9.7561e-05 g/l KI 4.87805e-05 g/l Na2WO4 x 2 H2O 3.90244e-06 g/l Na2SeO3 x 5 H2O 2.92683e-06 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2507 | positive | growth | 98 | hyperthermophilic |
67770 | positive | growth | 95 | hyperthermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 2507
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
2507 | black smoker at 2011 m depth | Gulf of California, Guaymas Basin | Mexico | MEX | North America |
67770 | Abyssal active 'black smoker' off Guaymas | Mexico | MEX | North America |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Hydrothermal vent |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 2507
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Pyrodictium abyssi DSM 6158 16S ribosomal RNA gene, partial sequence | KC145153 | 1307 | ena | 391605 |
20218 | Pyrodictium abyssi strain NBRC 100439 16S ribosomal RNA gene, partial sequence | KC139249 | 1317 | ena | 54256 |
2507 | P.abyssi 16S rRNA gene | X99559 | 1351 | ena | 391605 |
GC content
@ref | GC-content | method |
---|---|---|
2507 | 59.0 | |
67770 | 59.3 | thermal denaturation, midpoint method (Tm) |
67770 | 59 | high performance liquid chromatography (HPLC) |
External links
@ref: 2507
culture collection no.: DSM 6158, JCM 9394, ATCC 49828, NBRC 100439
straininfo link
- @ref: 82718
- straininfo: 42704
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2507 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6158) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6158 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
82718 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42704.1 | StrainInfo: A central database for resolving microbial strain identifiers |