Strain identifier

BacDive ID: 135242

Type strain: Yes

Species: Haemophilus haemolyticus

Strain Designation: AQ/3273

Strain history: CIP <- 1989, NCTC <- 1969, K. Zinneman, Leeds, UK <- D.C. Turk <- A.M. McIver, Newcastle, UK: strain AQ/3273

NCBI tax ID(s): 726 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 66335

BacDive-ID: 135242

DSM-Number: 103601

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Haemophilus haemolyticus AQ/3273 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from Human sputum,chest infection.

NCBI tax id

  • NCBI tax id: 726
  • Matching level: species

strain history

@refhistory
66335<- NCTC; NCTC 10659 <- K. Zinnemann; <- A.M. Iver; AQ/3273
34239CIP <- 1989, NCTC <- 1969, K. Zinneman, Leeds, UK <- D.C. Turk <- A.M. McIver, Newcastle, UK: strain AQ/3273

doi: 10.13145/bacdive135242.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Haemophilus
  • species: Haemophilus haemolyticus
  • full scientific name: Haemophilus haemolyticus Bergey et al. 1923 (Approved Lists 1980)

@ref: 66335

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Haemophilus

species: Haemophilus haemolyticus

full scientific name: Haemophilus haemolyticus Bergey et al. 1923

strain designation: AQ/3273

type strain: yes

Morphology

cell morphology

  • @ref: 34239
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34239MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
66335HAEMOPHILUS MEDIUM (DSMZ Medium 804)yesName: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/804
66335CHOCOLATE AGAR (DSMZ Medium 429b)yesName: CHOCOLATE AGAR (DSMZ Medium 429b) Composition: Horse blood 100.0 g/l Sheep blood 100.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/429b
34239CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
34239positivegrowth37mesophilic
45836positivegrowth37mesophilic
66335positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
45836microaerophile
34239facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3423917632nitrate+reduction
3423916301nitrite-reduction
6837717634D-glucose+builds acid from
6837715824D-fructose-builds acid from
6837717306maltose+builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis

metabolite production

  • @ref: 34239
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase+3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
34239oxidase-
34239catalase+1.11.1.6
34239urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34239-+---+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GAL
66335-+-+----+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
45836Human sputum,chest infectionNewcastleUnited KingdomGBREurope
66335human sputum, chest infection
34239Other, Sputum, chest infection

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Infection#Patient
#Host Body Product#Fluids#Sputum
#Host Body-Site#Other#Torso

taxonmaps

  • @ref: 69479
  • File name: preview.99_8861.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_30;97_1251;98_1502;99_8861&stattab=map
  • Last taxonomy: Haemophilus haemolyticus subclade
  • 16S sequence: NR_104930
  • Sequence Identity:
  • Total samples: 63
  • soil counts: 1
  • animal counts: 62

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
66335yes, in single cases1Risk group (German classification)
342392Risk group (French classification)

Sequence information

16S sequences

  • @ref: 66335
  • description: Haemophilus haemolyticus strain CIP 103290 16S ribosomal RNA, partial sequence
  • accession: NR_104930
  • length: 1486
  • database: nuccore
  • NCBI tax ID: 726

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haemophilus haemolyticus ATCC 33390GCA_004368535contigncbi726
66792Haemophilus haemolyticus CCUG 12834GCA_001679045scaffoldncbi726
66792Haemophilus haemolyticus strain ATCC 33390726.278wgspatric726
66792Haemophilus haemolyticus strain CCUG 12834726.59wgspatric726
66792Haemophilus haemolyticus strain NCTC10659726.279wgspatric726
66792Haemophilus haemolyticus CCUG 128342916272478draftimg726
66792Haemophilus haemolyticus AQ/3273, ATCC 333902531839471draftimg1095742

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno95.935no
gram-positiveno98.023no
anaerobicno92.789no
aerobicno96.382yes
halophileno53.669no
spore-formingno98.455no
glucose-utilno56.941no
thermophileno97.99no
motileno89.689no
glucose-fermentyes53.151no

External links

@ref: 66335

culture collection no.: CIP 103290, ATCC 33390, CCUG 12834, NCTC 10659, DSM 103601

straininfo link

  • @ref: 92638
  • straininfo: 40473

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity23315478Prevalence and mechanisms of beta-lactam resistance in Haemophilus haemolyticus.Witherden EA, Tristram SGJ Antimicrob Chemother10.1093/jac/dks5322013Adolescent, Adult, Anti-Bacterial Agents/*pharmacology, Child, Preschool, DNA, Bacterial/genetics, Female, Haemophilus/*drug effects/genetics, Haemophilus Infections/*epidemiology/microbiology, Humans, Infant, Male, Microbial Sensitivity Tests, Polymerase Chain Reaction, Prevalence, Young Adult, *beta-Lactam Resistance, beta-Lactams/*pharmacology
Pathogenicity24562614Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus.Witherden EA, Bajanca-Lavado MP, Tristram SG, Nunes AJ Antimicrob Chemother10.1093/jac/dku0222014Haemophilus/*drug effects/*genetics, Haemophilus Infections/microbiology, Haemophilus influenzae/*drug effects/*genetics, Humans, Penicillin Resistance/*genetics, Penicillin-Binding Proteins/*genetics, *Recombination, Genetic
Phylogeny32163029Haemophilus seminalis sp. nov., isolated from human semen.Zheng ML, Li LH, Liu B, Lin YB, Zhang XT, Chen C, Qu PH, Zeng JInt J Syst Evol Microbiol10.1099/ijsem.0.0040742020Bacterial Typing Techniques, Base Composition, Cadaverine/chemistry, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Haemophilus/*classification/isolation & purification, Humans, Male, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Semen/*microbiology, Sequence Analysis, DNAPathogenicity

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34239Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103290Collection of Institut Pasteur (CIP 103290)
45836Curators of the CCUGhttps://www.ccug.se/strain?id=12834Culture Collection University of Gothenburg (CCUG) (CCUG 12834)
66335Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103601Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103601)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
92638Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID40473.1