Strain identifier
BacDive ID: 135242
Type strain:
Species: Haemophilus haemolyticus
Strain Designation: AQ/3273
Strain history: CIP <- 1989, NCTC <- 1969, K. Zinneman, Leeds, UK <- D.C. Turk <- A.M. McIver, Newcastle, UK: strain AQ/3273
NCBI tax ID(s): 726 (species)
General
@ref: 66335
BacDive-ID: 135242
DSM-Number: 103601
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped, human pathogen
description: Haemophilus haemolyticus AQ/3273 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from Human sputum,chest infection.
NCBI tax id
- NCBI tax id: 726
- Matching level: species
strain history
@ref | history |
---|---|
66335 | <- NCTC; NCTC 10659 <- K. Zinnemann; <- A.M. Iver; AQ/3273 |
34239 | CIP <- 1989, NCTC <- 1969, K. Zinneman, Leeds, UK <- D.C. Turk <- A.M. McIver, Newcastle, UK: strain AQ/3273 |
doi: 10.13145/bacdive135242.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pasteurellales
- family: Pasteurellaceae
- genus: Haemophilus
- species: Haemophilus haemolyticus
- full scientific name: Haemophilus haemolyticus Bergey et al. 1923 (Approved Lists 1980)
@ref: 66335
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pasteurellales
family: Pasteurellaceae
genus: Haemophilus
species: Haemophilus haemolyticus
full scientific name: Haemophilus haemolyticus Bergey et al. 1923
strain designation: AQ/3273
type strain: yes
Morphology
cell morphology
- @ref: 34239
- gram stain: negative
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
34239 | MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml) | |
66335 | HAEMOPHILUS MEDIUM (DSMZ Medium 804) | yes | Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water | https://mediadive.dsmz.de/medium/804 |
66335 | CHOCOLATE AGAR (DSMZ Medium 429b) | yes | Name: CHOCOLATE AGAR (DSMZ Medium 429b) Composition: Horse blood 100.0 g/l Sheep blood 100.0 g/l Columbia agar base | https://mediadive.dsmz.de/medium/429b |
34239 | CIP Medium 10 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
34239 | positive | growth | 37 | mesophilic |
45836 | positive | growth | 37 | mesophilic |
66335 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
45836 | microaerophile |
34239 | facultative anaerobe |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
34239 | 17632 | nitrate | + | reduction |
34239 | 16301 | nitrite | - | reduction |
68377 | 17634 | D-glucose | + | builds acid from |
68377 | 15824 | D-fructose | - | builds acid from |
68377 | 17306 | maltose | + | builds acid from |
68377 | 17992 | sucrose | - | builds acid from |
68377 | 18257 | ornithine | - | degradation |
68377 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 34239
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68377 | beta-galactosidase | - | 3.2.1.23 |
68377 | alkaline phosphatase | + | 3.1.3.1 |
68377 | lipase | - | |
68377 | urease | - | 3.5.1.5 |
68377 | ornithine decarboxylase | - | 4.1.1.17 |
68377 | beta-lactamase | - | 3.5.2.6 |
34239 | oxidase | - | |
34239 | catalase | + | 1.11.1.6 |
34239 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
34239 | - | + | - | - | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API NH
@ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL |
---|---|---|---|---|---|---|---|---|---|---|
66335 | - | + | - | + | - | - | - | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
45836 | Human sputum,chest infection | Newcastle | United Kingdom | GBR | Europe |
66335 | human sputum, chest infection | ||||
34239 | Other, Sputum, chest infection |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | |
#Infection | #Patient | |
#Host Body Product | #Fluids | #Sputum |
#Host Body-Site | #Other | #Torso |
taxonmaps
- @ref: 69479
- File name: preview.99_8861.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_30;97_1251;98_1502;99_8861&stattab=map
- Last taxonomy: Haemophilus haemolyticus subclade
- 16S sequence: NR_104930
- Sequence Identity:
- Total samples: 63
- soil counts: 1
- animal counts: 62
Safety information
risk assessment
@ref | pathogenicity human | biosafety level | biosafety level comment |
---|---|---|---|
66335 | yes, in single cases | 1 | Risk group (German classification) |
34239 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 66335
- description: Haemophilus haemolyticus strain CIP 103290 16S ribosomal RNA, partial sequence
- accession: NR_104930
- length: 1486
- database: nuccore
- NCBI tax ID: 726
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Haemophilus haemolyticus ATCC 33390 | GCA_004368535 | contig | ncbi | 726 |
66792 | Haemophilus haemolyticus CCUG 12834 | GCA_001679045 | scaffold | ncbi | 726 |
66792 | Haemophilus haemolyticus strain ATCC 33390 | 726.278 | wgs | patric | 726 |
66792 | Haemophilus haemolyticus strain CCUG 12834 | 726.59 | wgs | patric | 726 |
66792 | Haemophilus haemolyticus strain NCTC10659 | 726.279 | wgs | patric | 726 |
66792 | Haemophilus haemolyticus CCUG 12834 | 2916272478 | draft | img | 726 |
66792 | Haemophilus haemolyticus AQ/3273, ATCC 33390 | 2531839471 | draft | img | 1095742 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 95.935 | no |
gram-positive | no | 98.023 | no |
anaerobic | no | 92.789 | no |
aerobic | no | 96.382 | yes |
halophile | no | 53.669 | no |
spore-forming | no | 98.455 | no |
glucose-util | no | 56.941 | no |
thermophile | no | 97.99 | no |
motile | no | 89.689 | no |
glucose-ferment | yes | 53.151 | no |
External links
@ref: 66335
culture collection no.: CIP 103290, ATCC 33390, CCUG 12834, NCTC 10659, DSM 103601
straininfo link
- @ref: 92638
- straininfo: 40473
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Pathogenicity | 23315478 | Prevalence and mechanisms of beta-lactam resistance in Haemophilus haemolyticus. | Witherden EA, Tristram SG | J Antimicrob Chemother | 10.1093/jac/dks532 | 2013 | Adolescent, Adult, Anti-Bacterial Agents/*pharmacology, Child, Preschool, DNA, Bacterial/genetics, Female, Haemophilus/*drug effects/genetics, Haemophilus Infections/*epidemiology/microbiology, Humans, Infant, Male, Microbial Sensitivity Tests, Polymerase Chain Reaction, Prevalence, Young Adult, *beta-Lactam Resistance, beta-Lactams/*pharmacology | |
Pathogenicity | 24562614 | Role of inter-species recombination of the ftsI gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in Haemophilus influenzae and Haemophilus haemolyticus. | Witherden EA, Bajanca-Lavado MP, Tristram SG, Nunes A | J Antimicrob Chemother | 10.1093/jac/dku022 | 2014 | Haemophilus/*drug effects/*genetics, Haemophilus Infections/microbiology, Haemophilus influenzae/*drug effects/*genetics, Humans, Penicillin Resistance/*genetics, Penicillin-Binding Proteins/*genetics, *Recombination, Genetic | |
Phylogeny | 32163029 | Haemophilus seminalis sp. nov., isolated from human semen. | Zheng ML, Li LH, Liu B, Lin YB, Zhang XT, Chen C, Qu PH, Zeng J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004074 | 2020 | Bacterial Typing Techniques, Base Composition, Cadaverine/chemistry, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Haemophilus/*classification/isolation & purification, Humans, Male, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Semen/*microbiology, Sequence Analysis, DNA | Pathogenicity |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
34239 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103290 | Collection of Institut Pasteur (CIP 103290) | |
45836 | Curators of the CCUG | https://www.ccug.se/strain?id=12834 | Culture Collection University of Gothenburg (CCUG) (CCUG 12834) | |
66335 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103601 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103601) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
68377 | Automatically annotated from API NH | |||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
92638 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID40473.1 |