Strain identifier

BacDive ID: 135237

Type strain: No

Species: Clostridioides difficile

Strain Designation: W 1194

Strain history: CIP <- 1996, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10131 Clostridium difficile A <- M. Delmée, Leuven, Belgium: strain W 1194

NCBI tax ID(s): 1496 (species)

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General

@ref: 34221

BacDive-ID: 135237

keywords: Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Clostridioides difficile W 1194 is an anaerobe, mesophilic, Gram-positive bacterium of the family Peptostreptococcaceae.

NCBI tax id

  • NCBI tax id: 1496
  • Matching level: species

strain history

  • @ref: 34221
  • history: CIP <- 1996, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10131 Clostridium difficile A <- M. Delmée, Leuven, Belgium: strain W 1194

doi: 10.13145/bacdive135237.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptostreptococcaceae
  • genus: Clostridioides
  • species: Clostridioides difficile
  • full scientific name: Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016

  • synonyms

    @refsynonym
    20215Bacillus difficilis
    20215Peptoclostridium difficile
    20215Clostridium difficile

@ref: 34221

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Peptostreptococcaceae

genus: Clostridioides

species: Clostridioides difficile

strain designation: W 1194

type strain: no

Morphology

cell morphology

  • @ref: 34221
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34221MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
34221CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
34221positivegrowth37mesophilic
53865positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53865anaerobe
34221anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3422117632nitrate-reduction
3422116301nitrite-reduction

metabolite production

  • @ref: 34221
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
34221oxidase-
34221catalase-1.11.1.6
34221urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34221--+--+--------------

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typegeographic location
34221BelgiumBELEurope
53865BelgiumBELEuropeHuman feces,patient with pseudomembranous colitisBrussels
34221BelgiumBELEuropeHuman, Feces

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host#Human
#Infection#Inflammation

Safety information

risk assessment

  • @ref: 34221
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 34221

culture collection no.: CIP 104903, ATCC 43594, CCUG 37779

straininfo link

  • @ref: 92633
  • straininfo: 41929

literature

Pubmed-IDtitleauthorsjournalDOIyearmesh
8914776Macrofragment localization of the toxin A and toxin B genes of Clostridium difficile.Norwood DA Jr, Sands JAClin Diagn Lab Immunol10.1128/cdli.3.6.782-785.19961996Chromosome Mapping/*methods, Clostridioides difficile/*genetics, Tetanus Toxin/*genetics
9281845Exosporial membrane plasticity of Clostridium sporogenes and Clostridium difficile.Panessa-Warren BJ, Tortora GT, Warren JBTissue Cell10.1016/s0040-8166(97)80031-61997Bacterial Adhesion, Cell Membrane/*ultrastructure, Clostridioides difficile/*cytology, Clostridium/*cytology, Microscopy, Electron, Scanning, Spores, Bacterial/cytology
9409783Physical map of the Clostridium difficile chromosome.Norwood DA Jr, Sands JAGene10.1016/s0378-1119(97)00443-51997*Chromosomes, Bacterial, Clostridioides difficile/*genetics, DNA Probes, *Restriction Mapping

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34221Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104903Collection of Institut Pasteur (CIP 104903)
53865Curators of the CCUGhttps://www.ccug.se/strain?id=37779Culture Collection University of Gothenburg (CCUG) (CCUG 37779)
68382Automatically annotated from API zym
92633Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID41929.1