Strain identifier

BacDive ID: 135234

Type strain: No

Species: Escherichia coli

Strain Designation: 2004/08351, Ec04-8351

Strain history: CIP <- 2011, FX Weill and M. Gouali, CNR E. coli Shigella, Inst. Pasteur, Paris, France: strain Ec04-8351

NCBI tax ID(s): 562 (species)

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General

@ref: 34217

BacDive-ID: 135234

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Escherichia coli 2004/08351 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from Stools.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 34217
  • history: CIP <- 2011, FX Weill and M. Gouali, CNR E. coli Shigella, Inst. Pasteur, Paris, France: strain Ec04-8351

doi: 10.13145/bacdive135234.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 34217

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

strain designation: 2004/08351, Ec04-8351

type strain: no

Morphology

cell morphology

  • @ref: 34217
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34217MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
34217CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

  • @ref: 34217
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 34217
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3421717632nitrate+reduction
3421716301nitrite+reduction

metabolite production

  • @ref: 34217
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
34217oxidase-
34217catalase+1.11.1.6
34217urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34217-+---+----++-+++----

Isolation, sampling and environmental information

isolation

  • @ref: 34217
  • sample type: Stools
  • isolation date: 2004

Safety information

risk assessment

  • @ref: 34217
  • biosafety level: 3
  • biosafety level comment: Risk group (French classification)

External links

@ref: 34217

culture collection no.: CIP 110347

straininfo link

  • @ref: 92630
  • straininfo: 395443

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34217Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110347Collection of Institut Pasteur (CIP 110347)
68382Automatically annotated from API zym
92630Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID395443.1