Strain identifier
BacDive ID: 13523
Type strain: 
Species: Pyrolobus fumarii
Strain Designation: 1A
Strain history: DSM 11204 <-- K. O. Stetter 1A.
NCBI tax ID(s): 694429 (strain), 54252 (species)
version 8.1 (current version)
General
@ref: 4234
BacDive-ID: 13523
DSM-Number: 11204
keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Pyrolobus fumarii 1A is an anaerobe, hyperthermophilic archaeon that was isolated from rock samples from the wall of a black smoker, 3650 m depth.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 694429 | strain |
| 54252 | species |
strain history
| @ref | history |
|---|---|
| 4234 | <- K.O. Stetter, 1A |
| 67770 | DSM 11204 <-- K. O. Stetter 1A. |
doi: 10.13145/bacdive13523.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Desulfurococcales
- family: Pyrodictiaceae
- genus: Pyrolobus
- species: Pyrolobus fumarii
- full scientific name: Pyrolobus fumarii Blöchl et al. 1999
@ref: 4234
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Desulfurococcales
family: Pyrodictiaceae
genus: Pyrolobus
species: Pyrolobus fumarii
full scientific name: Pyrolobus fumarii Blöchl et al. 1999
strain designation: 1A
type strain: yes
Morphology
multimedia
- @ref: 66793
- multimedia content: EM_DSM_11204_1.jpg
- caption: electron microscopic image
- intellectual property rights: © HZI/Manfred Rohde
Culture and growth conditions
culture temp
- @ref: 67770
- growth: positive
- type: growth
- temperature: 103
- range: hyperthermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4234
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | latitude | longitude |
|---|---|---|---|---|
| 4234 | rock samples from the wall of a black smoker, 3650 m depth | Mid-Atlantic Ridge, TAG hydrothermal vent site | 26 | -45 |
| 67770 | Hydrothermally heated black smoker wall at the TAG site at the Mid Atlantic Ridge (3,650 m in depth) |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Environmental | #Terrestrial | #Geologic |
Safety information
risk assessment
- @ref: 4234
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4234
- description: P.fumarius 16S rRNA gene
- accession: X99555
- length: 1372
- database: ena
- NCBI tax ID: 694429
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Pyrolobus fumarii 1A | GCA_000223395 | complete | ncbi | 694429 |
| 66792 | Pyrolobus fumarii 1A, DSM 11204 | 2505679005 | complete | img | 694429 |
| 66792 | Pyrolobus fumarii 1A | 694429.7 | complete | patric | 694429 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 4234 | 53 | thermal denaturation, midpoint method (Tm) |
| 67770 | 52.9 | high performance liquid chromatography (HPLC) |
| 67770 | 53.4 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 67.042 | no |
| flagellated | no | 93.455 | no |
| gram-positive | no | 97.182 | no |
| anaerobic | yes | 99.153 | yes |
| aerobic | no | 96.513 | yes |
| halophile | no | 87.742 | no |
| spore-forming | no | 96.017 | no |
| thermophile | yes | 100 | yes |
| glucose-util | yes | 73.591 | no |
| glucose-ferment | no | 62.598 | no |
External links
@ref: 4234
culture collection no.: DSM 11204, JCM 17356
straininfo link
- @ref: 82714
- straininfo: 49050
literature
- topic: Metabolism
- Pubmed-ID: 9680332
- title: Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit for life to 113 degrees C.
- authors: Blochl E, Rachel R, Burggraf S, Hafenbradl D, Jannasch HW, Stetter KO
- journal: Extremophiles
- DOI: 10.1007/s007920050010
- year: 1997
- mesh: Ammonia/metabolism, Archaea/*classification/genetics/metabolism/ultrastructure, Base Composition, DNA, Archaeal, Heat-Shock Proteins/analysis, Hydrogen/metabolism, Hydrogenase/metabolism, Nitrates/metabolism, Nucleic Acid Hybridization, Oxidation-Reduction, Pressure, Temperature, Thiosulfates/metabolism
- topic2: Phylogeny
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 4234 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11204) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11204 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 82714 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49050.1 | StrainInfo: A central database for resolving microbial strain identifiers |