Strain identifier

BacDive ID: 13519

Type strain: Yes

Species: Psychromonas ingrahamii

Strain Designation: 37

Variant: Isotype of BacDive ID 131084

Strain history: CIP <- 2005, P. Kämpfer, Giessen, Germany: strain 37

NCBI tax ID(s): 357794 (species)

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General

@ref: 7112

BacDive-ID: 13519

DSM-Number: 17664

keywords: 16S sequence, Bacteria, facultative anaerobe, psychrophilic, Gram-negative, rod-shaped

description: Psychromonas ingrahamii 37 is a facultative anaerobe, psychrophilic, Gram-negative bacterium that was isolated from Arctic polar sea ice.

NCBI tax id

  • NCBI tax id: 357794
  • Matching level: species

strain history

@refhistory
7112<- P. Kämpfer, Univ. Giessen, Germany; 37 <- J. T. Staley, Univ. of Washington, Dept. Microbiol., USA
383472005, P. Kämpfer, Giessen, Germany: strain 37
120115CIP <- 2005, P. Kämpfer, Giessen, Germany: strain 37

doi: 10.13145/bacdive13519.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Psychromonadaceae
  • genus: Psychromonas
  • species: Psychromonas ingrahamii
  • full scientific name: Psychromonas ingrahamii Auman et al. 2006

@ref: 7112

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Psychromonadaceae

genus: Psychromonas

species: Psychromonas ingrahamii

full scientific name: Psychromonas ingrahamii Auman et al. 2006

strain designation: 37

variant: Isotype of BacDive ID 131084

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31694negative06-14 µm1.25-1.5 µmrod-shapedno
120115negativerod-shapedno

pigmentation

  • @ref: 31694
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7112BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
38347Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120115CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
7112positivegrowth12psychrophilic
38347positivegrowth5psychrophilic
59343positivegrowth12psychrophilic
120115positivegrowth5-15psychrophilic
120115nogrowth18psychrophilic
120115nogrowth25mesophilic
120115nogrowth30mesophilic

culture pH

@refabilitytypepH
31694positivegrowth6.5-7.4
31694positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31694facultative anaerobe
59343aerobe
120115facultative anaerobe

spore formation

  • @ref: 31694
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31694NaClpositivegrowth01-20 %
31694NaClpositiveoptimum01-12 %
120115NaClnogrowth0 %
120115NaClnogrowth2 %
120115NaClnogrowth4 %
120115NaClnogrowth6 %
120115NaClnogrowth8 %
120115NaClnogrowth10 %

observation

  • @ref: 31694
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3169430089acetate+carbon source
3169435391aspartate+carbon source
3169417057cellobiose+carbon source
3169428757fructose+carbon source
316945417glucosamine+carbon source
3169417234glucose+carbon source
3169429987glutamate+carbon source
3169417754glycerol+carbon source
3169429864mannitol+carbon source
3169417272propionate+carbon source
3169415361pyruvate+carbon source
3169433942ribose+carbon source
3169417814salicin+carbon source
3169430031succinate+carbon source
3169417992sucrose+carbon source
3169427082trehalose+carbon source
3169417632nitrate+reduction
1201154853esculin-hydrolysis
12011517632nitrate+reduction
12011516301nitrite-reduction
12011517632nitrate-respiration

metabolite production

  • @ref: 120115
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31694catalase+1.11.1.6
31694cytochrome oxidase+1.9.3.1
120115oxidase+
120115beta-galactosidase+3.2.1.23
120115alcohol dehydrogenase-1.1.1.1
120115gelatinase+
120115amylase-
120115caseinase-3.4.21.50
120115catalase+1.11.1.6
120115tween esterase-
120115lecithinase-
120115lipase-
120115lysine decarboxylase-4.1.1.18
120115ornithine decarboxylase-4.1.1.17
120115protease-
120115tryptophan deaminase-
120115urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120115-++--+-++-++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7112Arctic polar sea iceAlaska, Point Barrow, Elson LagoonUSAUSANorth America
59343Sea iceAlaska,Point Barrow,Elson LagoonUSAUSANorth America
120115Environment, Artic sea iceAlaskaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Ice
#Condition#Psychrophilic (<10°C)
#Climate#Cold#Polar

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71121Risk group (German classification)
1201151Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7112
  • description: Psychromonas ingrahamii 37 16S ribosomal RNA gene, partial sequence
  • accession: U73721
  • length: 1469
  • database: ena
  • NCBI tax ID: 357804

GC content

@refGC-content
711240.0
3169440

External links

@ref: 7112

culture collection no.: DSM 17664, CCUG 51855, CIP 108865

straininfo link

  • @ref: 82710
  • straininfo: 291001

literature

  • topic: Phylogeny
  • Pubmed-ID: 16627645
  • title: Psychromonas ingrahamii sp. nov., a novel gas vacuolate, psychrophilic bacterium isolated from Arctic polar sea ice.
  • authors: Auman AJ, Breezee JL, Gosink JJ, Kampfer P, Staley JT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64068-0
  • year: 2006
  • mesh: Alaska, Arctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Environmental Microbiology, Fatty Acids/analysis/isolation & purification, Gammaproteobacteria/*classification/cytology/*isolation & purification/physiology, Genes, rRNA/genetics, Ice Cover/*microbiology, Microscopy, Electron, Transmission, Microscopy, Phase-Contrast, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymers/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/pharmacology, Temperature, Vacuoles
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7112Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17664)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17664
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31694Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2797128776041
38347Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6542
59343Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51855)https://www.ccug.se/strain?id=51855
68382Automatically annotated from API zym
82710Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID291001.1StrainInfo: A central database for resolving microbial strain identifiers
120115Curators of the CIPCollection of Institut Pasteur (CIP 108865)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108865