Strain identifier
BacDive ID: 13513
Type strain: 
Species: Actinophytocola corallinus
Strain Designation: ID 06-A0464
Strain history: <- NBRC; NBRC 105525 <- M. Otoguro(NITE) and Y. Widyastuti(LIPI); ID 06-A0464
NCBI tax ID(s): 657018 (species)
version 9.2 (current version)
General
@ref: 18016
BacDive-ID: 13513
DSM-Number: 45659
keywords: 16S sequence, Bacteria, mesophilic
description: Actinophytocola corallinus ID 06-A0464 is a mesophilic bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 657018
- Matching level: species
strain history
- @ref: 18016
- history: <- NBRC; NBRC 105525 <- M. Otoguro(NITE) and Y. Widyastuti(LIPI); ID 06-A0464
doi: 10.13145/bacdive13513.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Actinophytocola
- species: Actinophytocola corallinus
- full scientific name: Actinophytocola corallinus corrig. Otoguro et al. 2011
synonyms
- @ref: 20215
- synonym: Actinophytocola corallina
@ref: 18016
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Actinophytocola
species: Actinophytocola corallina
full scientific name: Actinophytocola corallina Otoguro et al. 2011
strain designation: ID 06-A0464
type strain: yes
Morphology
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 21438 | Sand yellow (1002) | 10-14 days | ISP 2 |
| 21438 | Ivory (1014) | 10-14 days | ISP 3 |
| 21438 | Light ivory (1015) | 10-14 days | ISP 4 |
| 21438 | Silk grey (7044) | 10-14 days | ISP 5 |
| 21438 | Cream (9001) | 10-14 days | ISP 7 |
| 21438 | Pebble grey (7032) | 10-14 days | Suter with tyrosine |
| 21438 | Cream (9001) | 10-14 days | Suter without tyrosine |
multimedia
- @ref: 18016
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45659.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 18016 | YEAST EXTRACT-STARCH AGAR (NBRC MED.266) (DSMZ Medium 1314) | yes | https://mediadive.dsmz.de/medium/1314 | Name: YEAST EXTRACT-STARCH AGAR (NBRC MED. 266) (DSMZ Medium 1314) Composition: Agar 15.0 g/l Starch 10.0 g/l Yeast extract 2.0 g/l Distilled water |
| 18016 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 21438 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 21438 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 21438 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 21438 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 21438 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 21438 | Suter with tyrosine | yes | Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes | |
| 21438 | Suter without tyrosine | yes | Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes |
culture temp
- @ref: 18016
- growth: positive
- type: growth
- temperature: 28
Physiology and metabolism
halophily
- @ref: 21438
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 7.5 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 21438 | 17234 | glucose | + | |
| 21438 | 22599 | arabinose | - | |
| 21438 | 17992 | sucrose | - | |
| 21438 | 18222 | xylose | - | |
| 21438 | 17268 | myo-inositol | - | |
| 21438 | 37684 | mannose | + | |
| 21438 | 28757 | fructose | + | |
| 21438 | 26546 | rhamnose | - | |
| 21438 | 16634 | raffinose | - | |
| 21438 | 62968 | cellulose | + | |
| 68379 | 28087 | glycogen | + | fermentation |
| 68379 | 17992 | sucrose | - | fermentation |
| 68379 | 17306 | maltose | - | fermentation |
| 68379 | 65327 | D-xylose | - | fermentation |
| 68379 | 16899 | D-mannitol | - | fermentation |
| 68379 | 16199 | urea | - | hydrolysis |
| 68379 | 4853 | esculin | - | hydrolysis |
| 68379 | 17716 | lactose | - | fermentation |
| 68379 | 16988 | D-ribose | - | fermentation |
| 68379 | 17634 | D-glucose | - | fermentation |
| 68379 | 5291 | gelatin | + | hydrolysis |
| 68379 | 17632 | nitrate | - | reduction |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | alpha-chymotrypsin | + | 3.4.21.1 |
| 68382 | trypsin | + | 3.4.21.4 |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | lipase (C 14) | - | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | esterase (C 4) | + | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68379 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | valine arylamidase | + | |
| 68379 | gelatinase | + | |
| 68379 | urease | - | 3.5.1.5 |
| 68379 | beta-glucosidase | - | 3.2.1.21 |
| 68379 | alkaline phosphatase | + | 3.1.3.1 |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
| 68379 | pyrazinamidase | - | 3.5.1.B15 |
API coryne
| @ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21438 | - | - | - | + | - | +/- | +/- | + | - | - | + | - | - | - | - | - | - | - | + |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21438 | + | + | + | - | + | + | - | + | + | + | + | +/- | + | - | + | + | + | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 18016
- sample type: soil
- geographic location: Lombok, Kuta
- country: Indonesia
- origin.country: IDN
- continent: Asia
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_40322.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_13737;97_16724;98_20762;99_40322&stattab=map
- Last taxonomy: Actinophytocola corallina
- 16S sequence: AB511316
- Sequence Identity:
- Total samples: 67
- soil counts: 53
- aquatic counts: 2
- animal counts: 8
- plant counts: 4
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 18016 | 1 | Risk group (German classification) |
| 21438 | L1 | German classification |
Sequence information
16S sequences
- @ref: 18016
- description: Actinophytocola corallina gene for 16S rRNA, partial sequence
- accession: AB511316
- length: 1472
- database: nuccore
- NCBI tax ID: 657018
GC content
- @ref: 18016
- GC-content: 71.2
External links
@ref: 18016
culture collection no.: DSM 45659, BTCC B-674, NBRC 105525
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 20495034 | Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil. | Otoguro M, Yamamura H, Tamura T, Irzaldi R, Ratnakomala S, Ridwan R, Kartina G, Triana E, Nurkanto A, Lestari Y, Lisdiyanti P, Widyastuti Y, Ando K | Int J Syst Evol Microbiol | 10.1099/ijs.0.023432-0 | 2010 | Actinomycetales/*classification/genetics/*isolation & purification/physiology, Arabinose/analysis, Base Composition, Cell Wall/chemistry, Cluster Analysis, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Galactose/analysis, Indonesia, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology | Genetics |
| Phylogeny | 24944338 | Actinophytocola gilvus sp. nov., isolated from desert soil crusts, and emended description of the genus Actinophytocola Indananda et al. 2010. | Sun HM, Zhang T, Yu LY, Lu XX, Mou XZ, Zhang YQ | Int J Syst Evol Microbiol | 10.1099/ijs.0.061051-0 | 2014 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 27151064 | Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil. | Bouznada K, Bouras N, Schumann P, Sproer C, Sabaou N, Klenk HP | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001136 | 2016 | Actinomycetales/*classification/genetics/isolation & purification, Africa, Northern, Algeria, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 18016 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45659) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45659 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 21438 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM45659.pdf | Compendium of Actinobacteria . HZI-Helmholtz-Centre for Infection Research, Braunschweig | |
| 68379 | Automatically annotated from API Coryne | |||
| 68382 | Automatically annotated from API zym | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta |