Strain identifier

BacDive ID: 13509

Type strain: Yes

Species: Allokutzneria albata

Strain Designation: R761-7

Strain history: ATCC 55061 <-- Bristol-Myers Squibb Co.; R761-7.

NCBI tax ID(s): 211114 (species)

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General

@ref: 11549

BacDive-ID: 13509

DSM-Number: 44149

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Allokutzneria albata R761-7 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 211114
  • Matching level: species

strain history

@refhistory
11549<- ATCC <- Bristol Meyers Co., R 761-7
67770ATCC 55061 <-- Bristol-Myers Squibb Co.; R761-7.

doi: 10.13145/bacdive13509.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Allokutzneria
  • species: Allokutzneria albata
  • full scientific name: Allokutzneria albata (Tomita et al. 1993) Labeda and Kroppenstedt 2008

  • synonyms

    • @ref: 20215
    • synonym: Kibdelosporangium albatum

@ref: 11549

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Allokutzneria

species: Allokutzneria albata

full scientific name: Allokutzneria albata (Tomita et al. 1993) Labeda and Kroppenstedt 2008 emend. Nouioui et al. 2018

strain designation: R761-7

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
20007Beige (1001)10-14 daysISP 2
20007Beige (1001)10-14 daysISP 3
20007Beige (1001)10-14 daysISP 4
20007Beige (1001)10-14 daysISP 5
20007Beige (1001)10-14 daysISP 6
20007Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
20007yesAerial MyceliumWhiteISP 2
20007yesAerial MyceliumWhiteISP 3
20007yesAerial MyceliumWhiteISP 4
20007yesAerial MyceliumWhiteISP 5
20007yesAerial MyceliumWhiteISP 6
20007yesAerial MyceliumWhiteISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11549GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20007ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20007ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20007ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20007ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20007ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20007ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
11549positivegrowth28mesophilic
20007positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 20007
  • spore description: Formation of spore chains, spore surface smooth
  • spore formation: yes

compound production

@refcompound
11549cycloviracins
20007Cycloviracin B

halophily

  • @ref: 20007
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4) (67%), MK-9(H2), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2000717234glucose+
2000722599arabinose+
2000717992sucrose+
2000718222xylose-
2000717268myo-inositol+
2000729864mannitol+
2000728757fructose+
2000726546rhamnose+
2000716634raffinose-
2000762968cellulose-
6836817632nitrate-reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
6836827897tryptophan-energy source

metabolite production

@refmetaboliteproductionChebi-ID
67770cycloviracin B1yes
67770cycloviracin B2yes
68368dinitrogenno17997
68368nitriteno16301
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368tryptophan deaminase-4.1.99.1
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382leucine arylamidase+3.4.11.1
68382acid phosphatase+3.1.3.2
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20007+++++++++++++-++++-
11549++/-+/--++/-+/--+/-++/--+-+/-+/-++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARANO2N2
20007+++++-+--++
11549+--------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11549soilMindanao IslandPhilippinesPHLAsia
67770SoilMindanao IslandPhilippinesPHLAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_930.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_549;97_634;98_732;99_930&stattab=map
  • Last taxonomy: Allokutzneria albata
  • 16S sequence: AJ512462
  • Sequence Identity:
  • Total samples: 944
  • soil counts: 861
  • aquatic counts: 23
  • animal counts: 33
  • plant counts: 27

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115491Risk group (German classification)
200071German classification

Sequence information

16S sequences

  • @ref: 11549
  • description: Kibdelosporangium albatum 16S rRNA gene, type strain DSM 44149T
  • accession: AJ512462
  • length: 1489
  • database: ena
  • NCBI tax ID: 211114

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Allokutzneria albata DSM 44149GCA_900103775chromosomencbi211114
66792Allokutzneria albata strain DSM 44149211114.4completepatric211114
66792Allokutzneria albata DSM 441492634166198draftimg211114
67770Allokutzneria albata NRRL B-24461GCA_000720955contigncbi211114

GC content

@refGC-contentmethod
1154971.6
6777071.7thermal denaturation, midpoint method (Tm)
6777070.3genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno88.675no
gram-positiveyes90.439no
anaerobicno98.791no
aerobicyes92.551no
halophileno85.987no
spore-formingyes94.452yes
glucose-utilyes88.677yes
flagellatedno96.309no
thermophileno98.602no
glucose-fermentno90.698yes

External links

@ref: 11549

culture collection no.: DSM 44149, ATCC 55061, JCM 9917, KCTC 9837, NBRC 101910, NRRL B-24461, CGMCC 4.1881, NCIMB 13433

straininfo link

  • @ref: 82701
  • straininfo: 43515

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1331014New antiviral antibiotics, cycloviracins B1 and B2. I. Production, isolation, physico-chemical properties and biological activity.Tsunakawa M, Komiyama N, Tenmyo O, Tomita K, Kawano K, Kotake C, Konishi M, Oki TJ Antibiot (Tokyo)10.7164/antibiotics.45.14671992Actinomycetales/*chemistry, Anti-Bacterial Agents/chemistry/*isolation & purification/pharmacology, Antiviral Agents/chemistry/*isolation & purification/pharmacology, *Macrolides, Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Simplexvirus/drug effectsEnzymology
Phylogeny8494740Kibdelosporangium albatum sp. nov., producer of the antiviral antibiotics cycloviracins.Tomita K, Hoshino Y, Miyaki TInt J Syst Bacteriol10.1099/00207713-43-2-2971993Actinomycetales/*classification/metabolism/ultrastructure, Anti-Bacterial Agents/biosynthesis, Antiviral Agents, Bacterial Typing Techniques, *MacrolidesMetabolism
Phylogeny18523197Proposal for the new genus Allokutzneria gen. nov. within the suborder Pseudonocardineae and transfer of Kibdelosporangium albatum Tomita et al. 1993 as Allokutzneria albata comb. nov.Labeda DP, Kroppenstedt RMInt J Syst Evol Microbiol10.1099/ijs.0.65474-02008Actinomycetales/*chemistry/*classification/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Molecular Sequence Data, Monosaccharides/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysisGenetics
Phylogeny23811139Allokutzneria multivorans sp. nov., an actinomycete isolated from soil.Cao YR, Chen X, Jiang Y, Wang LS, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.054411-02013Actinomycetales/*classification/genetics/isolation & purification, Base Composition, Carbohydrates/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny25052392Allokutzneria oryzae sp. nov., isolated from rhizospheric soil of Oryza sativa L.Duangmal K, Poomthongdee N, Pathom-Aree W, Take A, Thamchaipenet A, Matsumoto A, Takahashi YInt J Syst Evol Microbiol10.1099/ijs.0.065169-02014Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11549Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44149)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44149
20007Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44149.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82701Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43515.1StrainInfo: A central database for resolving microbial strain identifiers