Strain identifier

BacDive ID: 13499

Type strain: Yes

Species: Actinomycetospora chiangmaiensis

Strain history: DSM 45062 <-- J. Wiese <-- Y. Jiang YIM 0006.

NCBI tax ID(s): 1120948 (strain), 402650 (species)

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General

@ref: 12307

BacDive-ID: 13499

DSM-Number: 45062

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Actinomycetospora chiangmaiensis DSM 45062 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil sample from tropical rain forest.

NCBI tax id

NCBI tax idMatching level
402650species
1120948strain

strain history

@refhistory
12307<- J. Wiese, IFM Geomar, Kiel <- Y. Jiang
67770DSM 45062 <-- J. Wiese <-- Y. Jiang YIM 0006.

doi: 10.13145/bacdive13499.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinomycetospora
  • species: Actinomycetospora chiangmaiensis
  • full scientific name: Actinomycetospora chiangmaiensis Jiang et al. 2008

@ref: 12307

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Actinomycetospora

species: Actinomycetospora chiangmaiensis

full scientific name: Actinomycetospora chiangmaiensis Jiang et al. 2008 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 32109
  • gram stain: positive
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20161Melon yellow (1028)10-14 daysISP 2
20161Melon yellow (1028)10-14 daysISP 3
20161Melon yellow (1028)10-14 daysISP 4
20161Melon yellow (1028)10-14 daysISP 5
20161Melon yellow (1028)10-14 daysISP 6
20161Melon yellow (1028)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
20161noISP 2
20161noISP 3
20161noISP 4
20161noISP 5
20161noISP 6
20161noISP 7

pigmentation

  • @ref: 32109
  • production: yes

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_45062_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45062_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45062_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45062_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45062_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12307BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
12307GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20161ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20161ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20161ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20161ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20161ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20161ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
12307positivegrowth28
20161positiveoptimum28
67770positivegrowth28

Physiology and metabolism

oxygen tolerance

  • @ref: 32109
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
32109yes
69481yes100

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2016117234glucose+
2016122599arabinose-
2016117992sucrose+
2016118222xylose-
2016117268myo-inositol+
2016129864mannitol+
2016128757fructose+
2016126546rhamnose-
2016116634raffinose-
2016162968cellulose-
3210922653asparagine+carbon source
3210928757fructose+carbon source
3210917234glucose+carbon source
3210917754glycerol+carbon source
3210915428glycine+carbon source
3210927570histidine+carbon source
3210917716lactose+carbon source
3210926546rhamnose+carbon source
3210933942ribose+carbon source
3210930911sorbitol+carbon source
3210917992sucrose+carbon source
3210918222xylose+carbon source

enzymes

@refvalueactivityec
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20161-+++++---+----+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12307soil sample from tropical rain forestChiang MaiThailandTHAAsia
67770Soil from a tropical rainforestChiang MaiThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Climate#Hot#Tropical

taxonmaps

  • @ref: 69479
  • File name: preview.99_426.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_289;97_319;98_354;99_426&stattab=map
  • Last taxonomy: Actinomycetospora chiangmaiensis
  • 16S sequence: AM398646
  • Sequence Identity:
  • Total samples: 994
  • soil counts: 537
  • aquatic counts: 82
  • animal counts: 303
  • plant counts: 72

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
123071Risk group (German classification)
201611German classification

Sequence information

16S sequences

  • @ref: 12307
  • description: Actinostreptospora chiangmaiensis partial 16S rRNA gene, type strain YIM 0006T
  • accession: AM398646
  • length: 1476
  • database: nuccore
  • NCBI tax ID: 402650

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomycetospora chiangmaiensis DSM 450621120948.3wgspatric1120948
66792Actinomycetospora chiangmaiensis DSM 450622515154015draftimg1120948
67770Actinomycetospora chiangmaiensis DSM 45062GCA_000379625scaffoldncbi1120948

GC content

@refGC-contentmethod
1230769.03
3210969
6777069high performance liquid chromatography (HPLC)
6777074genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes89.049no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.539yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes76.584yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes87.418yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno93.9yes
69480flagellatedmotile2+Ability to perform flagellated movementno86.691yes

External links

@ref: 12307

culture collection no.: DSM 45062, JCM 15998, NBRC 104400, YIM 0006, CCTCC AA 205017, TBRC 8064

straininfo link

  • @ref: 82691
  • straininfo: 389478

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18218940Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae.Jiang Y, Wiese J, Tang SK, Xu LH, Imhoff JF, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.64976-02008Actinomycetales/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, Thailand, *Trees, *Tropical ClimateGenetics
Phylogeny21148678Actinomycetospora rishiriensis sp. nov., isolated from a lichen.Yamamura H, Ashizawa H, Nakagawa Y, Hamada M, Ishida Y, Otoguro M, Tamura T, Hayakawa MInt J Syst Evol Microbiol10.1099/ijs.0.028753-02010Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/genetics, Fatty Acids/metabolism, Japan, Lichens/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/metabolismMetabolism
Phylogeny21386850Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample.Yamamura H, Ashizawa H, Nakagawa Y, Hamada M, Ishida Y, Otoguro M, Tamura T, Hayakawa MJ Antibiot (Tokyo)10.1038/ja.2011.152011Actinobacteria/*classification/genetics/isolation & purification, *DNA, Bacterial, Japan, Lichens/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization/methods, Phenotype, Phylogeny, RNA, Bacterial, *RNA, Ribosomal, 16S, Sequence Analysis, RNAGenetics
Phylogeny24573328Actinomycetospora atypica sp. nov., a novel soil actinomycete and emended description of the genus Actinomycetospora.Zhang Y, Liu C, Zhang J, Shen Y, Li C, He H, Wang X, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-014-0143-22014Actinomycetales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny30059002Actinomycetospora endophytica sp. nov., isolated from wild orchid (Podochilus microphyllus Lindl.) in Thailand.Sakdapetsiri C, Ngaemthao W, Suriyachadkun C, Duangmal K, Kitpreechavanich VInt J Syst Evol Microbiol10.1099/ijsem.0.0029382018Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Orchidaceae/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Metabolism32351493Actinobacterial Degradation of 2-Hydroxyisobutyric Acid Proceeds via Acetone and Formyl-CoA by Employing a Thiamine-Dependent Lyase Reaction.Rohwerder T, Rohde MT, Jehmlich N, Purswani JFront Microbiol10.3389/fmicb.2020.006912020
Phylogeny35851075Actinomycetospora soli sp. nov., isolated from the rhizosphere soil of Averrhoa carambola L.Chantavorakit T, Duangmal KInt J Syst Evol Microbiol10.1099/ijsem.0.0052772022*Actinomycetales, *Averrhoa, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil, Soil Microbiology, ThailandTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
12307Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45062)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45062
20161Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45062.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32109Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2835428776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82691Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389478.1StrainInfo: A central database for resolving microbial strain identifiers