Strain identifier

BacDive ID: 134979

Type strain: No

Species: Pseudomonas putida

Strain history: CIP <- 1955, NCIB, Pseudomonas mildenbergii <- ATCC <- B.W. Hammer, Pseudomonas cyanogena

NCBI tax ID(s): 303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 33684

BacDive-ID: 134979

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas putida CIP 55.5 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

@refhistory
67770IAM 1505 <-- ATCC 795 <-- B. W. Hammer ("Pseudomonas cyanogena").
33684CIP <- 1955, NCIB, Pseudomonas mildenbergii <- ATCC <- B.W. Hammer, Pseudomonas cyanogena

doi: 10.13145/bacdive134979.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 33684

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

type strain: no

Morphology

cell morphology

  • @ref: 33684
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33684MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
33684CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
33684CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
33684positivegrowth30mesophilic
67770positivegrowth25mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 33684
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3368417632nitrate+reduction
3368416301nitrite-reduction

metabolite production

  • @ref: 33684
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
33684oxidase+
33684catalase+1.11.1.6
33684urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
33684-+++-+--+-++--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
33684IowaUSAUSANorth America
44479IowaUSAUSANorth AmericaBlue milk
67770Blue milk
33684IowaUnited States of AmericaUSANorth AmericaFood, Blue milk

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Host Body Product#Fluids#Milk

Safety information

risk assessment

  • @ref: 33684
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

  • @ref: 67770
  • description: Pseudomonas putida NRRL B-252
  • accession: GCA_005930545
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 303

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.815no
flagellatedyes83.173no
gram-positiveno98.423no
anaerobicno98.141no
aerobicyes95.619no
halophileno85.935no
spore-formingno95.498no
thermophileno99.828no
glucose-utilyes91.307no
glucose-fermentno89.732no

External links

@ref: 33684

culture collection no.: CIP 55.5, ATCC 795, IAM 1505, NCDO 754, NCIMB 8176, CCUG 2082, IFO 3773, NRRL B-252, NRRL B-21, JCM 20187

straininfo link

  • @ref: 92412
  • straininfo: 45460

literature

  • Pubmed-ID: 24415019
  • title: Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and Rhizopus oryzae.
  • authors: Pabai F, Kermasha S, Morin A
  • journal: World J Microbiol Biotechnol
  • DOI: 10.1007/BF00361014
  • year: 1995

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
33684Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2055.5Collection of Institut Pasteur (CIP 55.5)
44479Curators of the CCUGhttps://www.ccug.se/strain?id=2082Culture Collection University of Gothenburg (CCUG) (CCUG 2082)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
92412Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID45460.1