Strain identifier
BacDive ID: 134979
Type strain:
Species: Pseudomonas putida
Strain history: CIP <- 1955, NCIB, Pseudomonas mildenbergii <- ATCC <- B.W. Hammer, Pseudomonas cyanogena
NCBI tax ID(s): 303 (species)
General
@ref: 33684
BacDive-ID: 134979
keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Pseudomonas putida CIP 55.5 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
@ref | history |
---|---|
67770 | IAM 1505 <-- ATCC 795 <-- B. W. Hammer ("Pseudomonas cyanogena"). |
33684 | CIP <- 1955, NCIB, Pseudomonas mildenbergii <- ATCC <- B.W. Hammer, Pseudomonas cyanogena |
doi: 10.13145/bacdive134979.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 33684
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
type strain: no
Morphology
cell morphology
- @ref: 33684
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
33684 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
33684 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
33684 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
33684 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 25 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 33684
- oxygen tolerance: obligate aerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
33684 | 17632 | nitrate | + | reduction |
33684 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 33684
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
33684 | oxidase | + | |
33684 | catalase | + | 1.11.1.6 |
33684 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
33684 | - | + | + | + | - | + | - | - | + | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | geographic location | country | origin.country | continent | sample type |
---|---|---|---|---|---|
33684 | Iowa | USA | USA | North America | |
44479 | Iowa | USA | USA | North America | Blue milk |
67770 | Blue milk | ||||
33684 | Iowa | United States of America | USA | North America | Food, Blue milk |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Dairy product |
#Host Body Product | #Fluids | #Milk |
Safety information
risk assessment
- @ref: 33684
- biosafety level: 1
- biosafety level comment: Risk group (French classification)
Sequence information
Genome sequences
- @ref: 67770
- description: Pseudomonas putida NRRL B-252
- accession: GCA_005930545
- assembly level: contig
- database: ncbi
- NCBI tax ID: 303
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 91.815 | no |
flagellated | yes | 83.173 | no |
gram-positive | no | 98.423 | no |
anaerobic | no | 98.141 | no |
aerobic | yes | 95.619 | no |
halophile | no | 85.935 | no |
spore-forming | no | 95.498 | no |
thermophile | no | 99.828 | no |
glucose-util | yes | 91.307 | no |
glucose-ferment | no | 89.732 | no |
External links
@ref: 33684
culture collection no.: CIP 55.5, ATCC 795, IAM 1505, NCDO 754, NCIMB 8176, CCUG 2082, IFO 3773, NRRL B-252, NRRL B-21, JCM 20187
straininfo link
- @ref: 92412
- straininfo: 45460
literature
- Pubmed-ID: 24415019
- title: Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and Rhizopus oryzae.
- authors: Pabai F, Kermasha S, Morin A
- journal: World J Microbiol Biotechnol
- DOI: 10.1007/BF00361014
- year: 1995
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
33684 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2055.5 | Collection of Institut Pasteur (CIP 55.5) | |
44479 | Curators of the CCUG | https://www.ccug.se/strain?id=2082 | Culture Collection University of Gothenburg (CCUG) (CCUG 2082) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68382 | Automatically annotated from API zym | |||
92412 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID45460.1 |