Strain identifier

BacDive ID: 134825

Type strain: No

Species: Lactococcus lactis

Strain Designation: N34, RICE

Strain history: CIP <- 1970, P. Cayeux, Inst. Pasteur, Paris, France: strain N34 <- Nat. Inst. Res. Dairying, Shinfield, UK: strain RICE

NCBI tax ID(s): 1358 (species)

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General

@ref: 33475

BacDive-ID: 134825

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Lactococcus lactis N34 is a facultative anaerobe, mesophilic, Gram-positive bacterium of the family Streptococcaceae.

NCBI tax id

  • NCBI tax id: 1358
  • Matching level: species

strain history

  • @ref: 33475
  • history: CIP <- 1970, P. Cayeux, Inst. Pasteur, Paris, France: strain N34 <- Nat. Inst. Res. Dairying, Shinfield, UK: strain RICE

doi: 10.13145/bacdive134825.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Lactococcus
  • species: Lactococcus lactis
  • full scientific name: Lactococcus lactis (Lister 1873) Schleifer et al. 1986

  • synonyms

    @refsynonym
    20215Streptococcus lactis subsp. diacetilactis
    20215Bacterium lactis
    20215Streptococcus lactis
    20215Lactobacillus xylosus

@ref: 33475

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Lactococcus

species: Lactococcus lactis

strain designation: N34, RICE

type strain: no

Morphology

cell morphology

  • @ref: 33475
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 33475

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33475MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
33475CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
33475positivegrowth30
33475positivegrowth10-41
33475nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 33475
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 33475
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
334754853esculin+hydrolysis
33475606565hippurate+hydrolysis
3347517632nitrate-reduction
3347516301nitrite-reduction
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837112936D-galactose+builds acid from
6837117754glycerol+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from

metabolite tests

  • @ref: 33475
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
33475oxidase-
33475beta-galactosidase-3.2.1.23
33475alcohol dehydrogenase+1.1.1.1
33475catalase-1.11.1.6
33475gamma-glutamyltransferase-2.3.2.2
33475lysine decarboxylase-4.1.1.18
33475ornithine decarboxylase-4.1.1.17
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase+3.2.1.21
68382lipase (C 14)-

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
33475--++-+----++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
33475+---+----++++--------+++++++---+---++-+----------

Safety information

risk assessment

  • @ref: 33475
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 33475

culture collection no.: CIP 70.57

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
33475Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2070.57Collection of Institut Pasteur (CIP 70.57)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym