Strain identifier

BacDive ID: 134770

Type strain: No

Species: Vibrio cholerae

Strain Designation: O14

Strain history: CIP <- 1962, J. Gallut: strain O14 <- Cholera Lab., Bangkok, Thailand

NCBI tax ID(s): 666 (species)

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General

@ref: 33413

BacDive-ID: 134770

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Vibrio cholerae O14 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Vibrionaceae.

NCBI tax id

  • NCBI tax id: 666
  • Matching level: species

strain history

  • @ref: 33413
  • history: CIP <- 1962, J. Gallut: strain O14 <- Cholera Lab., Bangkok, Thailand

doi: 10.13145/bacdive134770.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio cholerae
  • full scientific name: Vibrio cholerae Pacini 1854 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio albensis

@ref: 33413

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales

family: Vibrionaceae

genus: Vibrio

species: Vibrio cholerae

strain designation: O14

type strain: no

Morphology

cell morphology

  • @ref: 33413
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33413MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
33413CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
33413positivegrowth30
33413positivegrowth15-41
33413nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 33413
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
33413NaClpositivegrowth0-6 %
33413NaClnogrowth8 %
33413NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3341316947citrate+carbon source
334134853esculin-hydrolysis
3341317234glucose+fermentation
3341317716lactose+fermentation
3341317632nitrate+reduction
3341316301nitrite-reduction
33413132112sodium thiosulfate-builds gas from
3341317234glucose+degradation
3341317632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837112936D-galactose+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

antibiotic resistance

  • @ref: 33413
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 33413
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3341315688acetoin+
3341317234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase (C 4)+
33413oxidase+
33413beta-galactosidase+3.2.1.23
33413alcohol dehydrogenase-1.1.1.1
33413gelatinase+
33413amylase+
33413DNase+
33413caseinase+3.4.21.50
33413catalase+1.11.1.6
33413tween esterase+
33413gamma-glutamyltransferase+2.3.2.2
33413lecithinase+
33413lipase+
33413lysine decarboxylase+4.1.1.18
33413ornithine decarboxylase+4.1.1.17
33413protease+
33413tryptophan deaminase-
33413urease-3.5.1.5
68382trypsin-3.4.21.4
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
33413-+++++----++-+---+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
33413+---+----++++----+---+-----++-++---++---------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
33413++-++--+-++------+-+-----------+-+---------------++--+-----++--------------+---++-+---+--+++-++---+

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentisolation date
33413ThailandTHAAsia
33413ThailandTHAAsia1959

Safety information

risk assessment

  • @ref: 33413
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 33413

culture collection no.: CIP 62.16

straininfo link

  • @ref: 92232
  • straininfo: 70603

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
33413Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2062.16Collection of Institut Pasteur (CIP 62.16)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
92232Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID70603.1