Strain identifier

BacDive ID: 13471

Type strain: Yes

Species: Saccharopolyspora rosea

Strain history: CCUG 56401 <-- A. F. Yassin IMMIB L-1070.

NCBI tax ID(s): 524884 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15805

BacDive-ID: 13471

DSM-Number: 45226

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Saccharopolyspora rosea DSM 45226 is an aerobe, spore-forming, mesophilic human pathogen that was isolated from bronchial lavage of a patient with bronchial carcinoma.

NCBI tax id

  • NCBI tax id: 524884
  • Matching level: species

strain history

@refhistory
15805<- A. F. Yassin, Univ. Bonn; IMMIB L-1070
67770CCUG 56401 <-- A. F. Yassin IMMIB L-1070.

doi: 10.13145/bacdive13471.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Saccharopolyspora
  • species: Saccharopolyspora rosea
  • full scientific name: Saccharopolyspora rosea Yassin 2009

@ref: 15805

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Saccharopolyspora

species: Saccharopolyspora rosea

full scientific name: Saccharopolyspora rosea Yassin 2009

type strain: yes

Morphology

cell morphology

  • @ref: 29063
  • gram stain: positive
  • cell shape: rod-shaped

colony morphology

@refcolony colorincubation periodmedium used
20183Sand yellow (1002)10-14 daysISP 2
20183Ivory (1014)10-14 daysISP 3
20183Beige (1001)10-14 daysISP 4
20183Ivory (1014)10-14 daysISP 5
20183Ivory (1014)10-14 daysISP 6
20183Light ivory (1015)10-14 daysISP 7
609442-3 days

multicellular morphology

@refforms multicellular complexmedium name
20183noISP 2
20183noISP 3
20183noISP 4
20183noISP 5
20183noISP 6
20183noISP 7

pigmentation

  • @ref: 29063
  • production: yes

multimedia

  • @ref: 15805
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45226.jpg
  • caption: Medium 553 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15805GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
15805GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20183ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20183ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20183ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20183ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20183ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20183ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15805positivegrowth28mesophilic
20183positiveoptimum28mesophilic
29063positivegrowth22-42
29063positiveoptimum32mesophilic
60944positivegrowth30-37mesophilic
67770positivegrowth35mesophilic

culture pH

@refabilitytypepH
29063positivegrowth06-08
29063positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29063aerobe
60944aerobe

spore formation

  • @ref: 29063
  • spore formation: yes

observation

@refobservation
29063aggregates in chains
67770quinones: MK-9(H4), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2018317234glucose+
2018322599arabinose+/-
2018317992sucrose+/-
2018318222xylose+/-
2018317268myo-inositol-
2018329864mannitol-
2018328757fructose-
2018326546rhamnose-
2018316634raffinose-
2018362968cellulose+
29063620642,3-butanediol+carbon source
2906330089acetate+carbon source
2906317128adipate+carbon source
2906315963ribitol+carbon source
2906316449alanine+carbon source
2906322599arabinose+carbon source
2906329016arginine+carbon source
2906316947citrate+carbon source
2906328260galactose+carbon source
290635291gelatin+carbon source
2906324265gluconate+carbon source
2906317234glucose+carbon source
2906324996lactate+carbon source
2906317306maltose+carbon source
2906329864mannitol+carbon source
2906317268myo-inositol+carbon source
2906318257ornithine+carbon source
2906326271proline+carbon source
2906317822serine+carbon source
2906330911sorbitol+carbon source
2906317992sucrose+carbon source
2906327082trehalose+carbon source
2906318222xylose+carbon source
290634853esculin+hydrolysis

enzymes

@refvalueactivityec
29063catalase+1.11.1.6
29063gelatinase+
29063urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20183+++-+----++----+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
15805bronchial lavage of a patient with bronchial carcinomaAachenGermanyDEUEurope
60944Human bronchial lavage,bronchial carcinomaAachenGermanyDEUEurope1993
67770Bronchial lavage from a 45-year-old patient with bronchial carcinoma

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Oral cavity and airways
#Host Body Product#Oral cavity and Airways#Bronchial wash

taxonmaps

  • @ref: 69479
  • File name: preview.99_110435.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1393;97_56585;98_74960;99_110435&stattab=map
  • Last taxonomy: Saccharopolyspora rosea subclade
  • 16S sequence: AM992060
  • Sequence Identity:
  • Total samples: 214
  • soil counts: 50
  • aquatic counts: 38
  • animal counts: 114
  • plant counts: 12

Safety information

risk assessment

  • @ref: 15805
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15805
  • description: Saccharopolyspora rosea partial 16S rRNA gene, type strain IMMIB L-1070T
  • accession: AM992060
  • length: 1492
  • database: ena
  • NCBI tax ID: 524884

External links

@ref: 15805

culture collection no.: DSM 45226, CCUG 56401, IMMIB L-1070, JCM 19121

straininfo link

  • @ref: 82663
  • straininfo: 398606

literature

  • topic: Phylogeny
  • Pubmed-ID: 19406809
  • title: Saccharopolyspora rosea sp. nov., isolated from a patient with bronchial carcinoma.
  • authors: Yassin AF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.005249-0
  • year: 2009
  • mesh: Bacterial Typing Techniques, Carcinoma, Bronchogenic/*microbiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Gram-Positive Bacterial Infections/*microbiology, Humans, Lung Neoplasms/*microbiology, Middle Aged, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/*classification/genetics/isolation & purification/physiology, Sequence Analysis, DNA, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15805Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45226)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45226
20183Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45226.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29063Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2549328776041
60944Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56401)https://www.ccug.se/strain?id=56401
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82663Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID398606.1StrainInfo: A central database for resolving microbial strain identifiers