Strain identifier
BacDive ID: 134703
Type strain:
Species: Clostridioides difficile
Strain history: CIP <- 2006, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 162-05, Clostridium difficile <- Montpellier Hosp., Montpellier, France
NCBI tax ID(s): 1496 (species)
General
@ref: 33285
BacDive-ID: 134703
keywords: Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped
description: Clostridioides difficile CIP 109239 is an anaerobe, mesophilic, Gram-positive bacterium of the family Peptostreptococcaceae.
NCBI tax id
- NCBI tax id: 1496
- Matching level: species
strain history
- @ref: 33285
- history: CIP <- 2006, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 162-05, Clostridium difficile <- Montpellier Hosp., Montpellier, France
doi: 10.13145/bacdive134703.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Peptostreptococcaceae
- genus: Clostridioides
- species: Clostridioides difficile
- full scientific name: Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016
synonyms
@ref synonym 20215 Bacillus difficilis 20215 Peptoclostridium difficile 20215 Clostridium difficile
@ref: 33285
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Eubacteriales
family: Peptostreptococcaceae
genus: Clostridioides
species: Clostridioides difficile
type strain: no
Morphology
cell morphology
- @ref: 33285
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 33285
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
33285 | MEDIUM 20 - for Anaerobic bacteria | yes | Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml) | |
33285 | CIP Medium 20 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20 | |
33285 | CIP Medium 45 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45 |
culture temp
- @ref: 33285
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 33285
- oxygen tolerance: anaerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
33285 | 17632 | nitrate | - | reduction |
33285 | 16301 | nitrite | + | reduction |
metabolite production
- @ref: 33285
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
33285 | oxidase | - | |
33285 | alcohol dehydrogenase | - | 1.1.1.1 |
33285 | catalase | - | 1.11.1.6 |
33285 | lysine decarboxylase | - | 4.1.1.18 |
33285 | ornithine decarboxylase | - | 4.1.1.17 |
33285 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
33285 | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | geographic location | country | origin.country | continent | sample type | isolation date |
---|---|---|---|---|---|---|
33285 | Montpellier | France | FRA | Europe | ||
33285 | Montpellier | France | FRA | Europe | Faeces | 2005 |
Safety information
risk assessment
- @ref: 33285
- biosafety level: 2
- biosafety level comment: Risk group (French classification)
External links
@ref: 33285
culture collection no.: CIP 109239
straininfo link
- @ref: 92173
- straininfo: 361808
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
33285 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109239 | Collection of Institut Pasteur (CIP 109239) | |
68382 | Automatically annotated from API zym | |||
92173 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID361808.1 |