Strain identifier

BacDive ID: 134627

Type strain: No

Species: Clostridium perfringens

Strain Designation: 77.2000

Strain history: CIP <- 2000, M.R. Popoff, Inst. Pasteur, Paris, France: strain 77.2000

NCBI tax ID(s): 1502 (species)

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General

@ref: 33076

BacDive-ID: 134627

keywords: Bacteria, mesophilic, Gram-positive, rod-shaped

description: Clostridium perfringens 77.2000 is a mesophilic, Gram-positive, rod-shaped bacterium of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

@refhistory
330762000, M.R. Popoff, Inst. Pasteur, Paris, France: strain 77.2000
33076CIP <- 2000, M.R. Popoff, Inst. Pasteur, Paris, France: strain 77.2000

doi: 10.13145/bacdive134627.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 33076

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

strain designation: 77.2000

type strain: no

Morphology

cell morphology

  • @ref: 33076
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 33076
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33076MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
33076CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 33076
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3307617108D-arabinose-degradation
3307615824D-fructose+/-degradation
3307617634D-glucose+/-degradation
3307617057cellobiose-degradation
3307617716lactose+/-degradation
3307617306maltose+/-degradation
3307617814salicin-degradation
3307617992sucrose+/-degradation
330764853esculin-hydrolysis
3307617632nitrate-reduction
3307616301nitrite-reduction
3307617632nitrate+respiration

metabolite production

  • @ref: 33076
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
33076oxidase-
33076beta-galactosidase+3.2.1.23
33076gelatinase+/-
33076amylase+
33076DNase+
33076caseinase+3.4.21.50
33076catalase-1.11.1.6
33076tween esterase-
33076lecithinase+
33076lipase-
33076protease-
33076urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
33076-++-----+-++++++-+--

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typeisolation date
33076FranceFRAEurope
33076FranceFRAEuropeYoung pig2000

Safety information

risk assessment

  • @ref: 33076
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 33076

culture collection no.: CIP 106526

straininfo link

  • @ref: 92117
  • straininfo: 67912

literature

  • topic: Pathogenicity
  • Pubmed-ID: 16398335
  • title: Survival of clostridium perfringens during simulated transport and stability of some plasmid-borne toxin genes under aerobic conditions.
  • authors: Johansson A, Engstrom BE, Frey J, Johansson KE, Baverud V
  • journal: Acta Vet Scand
  • DOI: 10.1186/1751-0147-46-241
  • year: 2005
  • mesh: Clostridium perfringens/genetics/*growth & development, Enterotoxins/*genetics, Oxygen/metabolism, *Plasmids, Temperature, Time Factors, Transportation
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
33076Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106526Collection of Institut Pasteur (CIP 106526)
68382Automatically annotated from API zym
92117Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID67912.1