Strain identifier

BacDive ID: 134599

Type strain: No

Species: Clostridium perfringens

Strain Designation: 3462000

Strain history: CIP <- 2001, M.R. Popoff, Inst. Pasteur, Paris, France: strain 346 2000

NCBI tax ID(s): 1502 (species)

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General

@ref: 33023

BacDive-ID: 134599

keywords: Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Clostridium perfringens 3462000 is an anaerobe, mesophilic, Gram-positive bacterium of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

@refhistory
330232001, M.R. Popoff, Inst. Pasteur, Paris, France: strain 346 2000
33023CIP <- 2001, M.R. Popoff, Inst. Pasteur, Paris, France: strain 346 2000

doi: 10.13145/bacdive134599.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 33023

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

strain designation: 3462000

type strain: no

Morphology

cell morphology

  • @ref: 33023
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 33023
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33023MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
33023CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
33023CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 33023
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 33023
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
330234853esculin-hydrolysis
3302317632nitrate-reduction
3302316301nitrite-reduction
3302317632nitrate+respiration

metabolite production

  • @ref: 33023
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
33023oxidase-
33023beta-galactosidase+3.2.1.23
33023gelatinase+/-
33023amylase+
33023DNase+
33023caseinase+3.4.21.50
33023catalase-1.11.1.6
33023tween esterase+
33023lecithinase+
33023lipase-
33023protease-
33023urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
33023-+++------+-++-+-+--

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
33023JanzeFranceFRAEurope
33023JanzéFranceFRAEuropeAnimal, Rabbit, intestine2000

Safety information

risk assessment

  • @ref: 33023
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 33023

culture collection no.: CIP 106943

straininfo link

  • @ref: 92098
  • straininfo: 101435

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
33023Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106943Collection of Institut Pasteur (CIP 106943)
68382Automatically annotated from API zym
92098Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID101435.1