Strain identifier

BacDive ID: 134571

Type strain: No

Species: Clostridium perfringens

Strain Designation: 3702/49

Strain history: CIP <- 2000, ATCC <- NCTC <- B. Hobbs: strain 3702/49

NCBI tax ID(s): 1502 (species)

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General

@ref: 32980

BacDive-ID: 134571

keywords: genome sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive

description: Clostridium perfringens 3702/49 is an anaerobe, spore-forming, mesophilic bacterium of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

  • @ref: 32980
  • history: CIP <- 2000, ATCC <- NCTC <- B. Hobbs: strain 3702/49

doi: 10.13145/bacdive134571.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 32980

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

strain designation: 3702/49

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
32980positiverod-shapedno

colony morphology

  • @ref: 32980
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
32980MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
32980CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

  • @ref: 32980
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 69480
  • oxygen tolerance: anaerobe
  • confidence: 99.588

spore formation

@refspore formationconfidence
69481yes100
69480yes99.689

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3298017108D-arabinose-degradation
3298015824D-fructose+/-degradation
3298017634D-glucose+/-degradation
3298065327D-xylose-degradation
3298017057cellobiose-degradation
3298017716lactose+/-degradation
3298017306maltose+/-degradation
3298017814salicin-degradation
3298017992sucrose+/-degradation
329804853esculin-hydrolysis
3298017632nitrate-reduction
3298016301nitrite+reduction
3298017632nitrate+respiration

metabolite production

  • @ref: 32980
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32980oxidase-
32980beta-galactosidase+3.2.1.23
32980gelatinase+/-
32980amylase+
32980DNase+
32980caseinase+3.4.21.50
32980catalase-1.11.1.6
32980tween esterase-
32980lecithinase+
32980lipase-
32980protease-
32980urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
32980-+++------++++-+-+--

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
32980LondonUnited KingdomGBREurope
32980LondonUnited KingdomGBREuropeFood, Stewed steak1950

Safety information

risk assessment

  • @ref: 32980
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium perfringens NCTC8359GCA_900461145contigncbi1502
66792Clostridium perfringens strain NCTC83591502.1042wgspatric1502

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno83.906no
gram-positiveyes96.135no
anaerobicyes97.862no
aerobicno95.3no
halophileno70.148no
spore-formingyes91.484no
thermophileno97.406no
glucose-utilyes90.161no
flagellatedno91.569no
glucose-fermentyes77.707no

External links

@ref: 32980

culture collection no.: CIP 106516, ATCC 12915, NCTC 8359

straininfo link

  • @ref: 92077
  • straininfo: 45531

literature

Pubmed-IDtitleauthorsjournalDOIyeartopicmeshtopic2
16701530Inhibition of Clostridium perfringens sporulation by Bacteroides fragilis and short-chain fatty acids.Wrigley DMAnaerobe10.1016/j.anaerobe.2004.05.0062004
24215704Inhibition of Clostridium perfringens growth by potassium lactate during an extended cooling of cooked uncured ground turkey breasts.Kennedy KM, Milkowski AL, Glass KAJ Food Prot10.4315/0362-028X.JFP-13-1062013PathogenicityAnimals, Clostridium perfringens/drug effects/growth & development/physiology, Cold Temperature, Colony Count, Microbial, Dose-Response Relationship, Drug, Food Contamination/analysis/prevention & control, Food Handling/methods, Food Microbiology, Food Preservation/*methods, Food Preservatives/*pharmacology, Humans, Poultry Products/*microbiology, Sodium Lactate/pharmacology, Turkeys, United StatesBiotechnology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
32980Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106516Collection of Institut Pasteur (CIP 106516)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
92077Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID45531.1