Strain identifier

BacDive ID: 134453

Type strain: No

Species: Pseudomonas aeruginosa

Strain history: CIP <- 1993, ATCC <- NCTC <- P. Waterworth, London, UK

NCBI tax ID(s): 287 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 32781

BacDive-ID: 134453

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Pseudomonas aeruginosa CIP 103837 is an obligate aerobe, mesophilic, motile bacterium that was isolated from Human.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 32781
  • history: CIP <- 1993, ATCC <- NCTC <- P. Waterworth, London, UK

doi: 10.13145/bacdive134453.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 32781

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes99.155
6948099.996negative
32781yesnegativerod-shaped

pigmentation

  • @ref: 32781
  • production: yes
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
32781MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
32781CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
32781positivegrowth30mesophilic
32781positivegrowth25-41
32781nogrowth5psychrophilic
32781nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 32781
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no98
69480no99.938

halophily

@refsaltgrowthtested relationconcentration
32781NaClpositivegrowth0-6 %
32781NaClnogrowth8 %
32781NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3278116947citrate-carbon source
327814853esculin-hydrolysis
3278117632nitrate+reduction
3278116301nitrite+reduction
3278115882phenol+degradation
3278117632nitrate+respiration

antibiotic resistance

  • @ref: 32781
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 32781
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32781oxidase+
32781beta-galactosidase-3.2.1.23
32781alcohol dehydrogenase+1.1.1.1
32781gelatinase+
32781amylase-
32781DNase-
32781caseinase+3.4.21.50
32781catalase+1.11.1.6
32781tween esterase-
32781lecithinase-
32781lipase-
32781lysine decarboxylase-4.1.1.18
32781ornithine decarboxylase-4.1.1.17
32781protease-
32781tryptophan deaminase-
32781urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
32781-+++++--+-++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
32781++-----------------+------+----+-+--------------+++--+--+-+++-++++-+---++++++++++++++--++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
44370Human
32781Human1968

isolation source categories

  • Cat1: #Host
  • Cat2: #Human

Safety information

risk assessment

  • @ref: 32781
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa NCTC10662GCA_900706875contigncbi287
66792Pseudomonas aeruginosa strain NCTC10662287.9424wgspatric287

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
gram-positiveno98.385no
anaerobicno98.086no
halophileno90.229no
spore-formingno93.192no
glucose-utilyes89.066no
motileyes90.023no
flagellatedyes85.623no
aerobicyes92.611no
thermophileno99.869yes
glucose-fermentno89.421no

External links

@ref: 32781

culture collection no.: CIP 103837, ATCC 25668, CCTM La 2767, NCTC 10662, CECT 118, NCIMB 13063, CCM 3989, WDCM 00114, CCUG 1423

straininfo link

  • @ref: 91977
  • straininfo: 376990

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
3182460Resistance studies with ofloxacin.Felmingham D, Foxall P, O'Hare MD, Webb G, Ghosh G, Gruneberg RNJ Antimicrob Chemother10.1093/jac/22.supplement_c.271988Drug Resistance, Microbial, Mutation, Ofloxacin/*pharmacology
6821456Carbenicillin resistance of Pseudomonas aeruginosa.Rodriguez-Tebar A, Rojo F, Damaso D, Vazquez DAntimicrob Agents Chemother10.1128/AAC.22.2.2551982*Bacterial Proteins, Carbenicillin/metabolism/*pharmacology, Carrier Proteins/isolation & purification/metabolism, Cell Membrane/metabolism, Cell Membrane Permeability, Electrophoresis, Polyacrylamide Gel, *Hexosyltransferases, Humans, In Vitro Techniques, Kinetics, Muramoylpentapeptide Carboxypeptidase/isolation & purification/metabolism, Penicillin Resistance, Penicillin-Binding Proteins, *Peptidyl Transferases, Pseudomonas aeruginosa/*drug effects/isolation & purification/metabolism, beta-Lactamases/metabolismEnzymologyMetabolism
8360125Accumulation and killing kinetics of fifteen quinolones for Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.Asuquo AE, Piddock LJJ Antimicrob Chemother10.1093/jac/31.6.8651993Anti-Infective Agents/chemistry/metabolism/*pharmacology, Escherichia coli/*drug effects, Fluoroquinolones, Kinetics, Microbial Sensitivity Tests, Pseudomonas aeruginosa/*drug effects, Solubility, Staphylococcus aureus/*drug effects, TemperatureEnzymologyPathogenicity
8405933An inexpensive infrared growth sensor array for detection of bacterial antibiotic susceptibility.Mason DJ, Lloyd AL, Lloyd DFEMS Microbiol Lett10.1111/j.1574-6968.1993.tb06394.x1993Ampicillin/pharmacology, Bacteria/*drug effects/isolation & purification, Ciprofloxacin/pharmacology, Escherichia coli/drug effects, Evaluation Studies as Topic, Gentamicins/pharmacology, Humans, Infrared Rays, Microbial Sensitivity Tests/*instrumentation, Penicillins/pharmacology, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effectsPathogenicityEnzymology
8586791Contamination of multi-use closed tracheal suction catheters: an in-vitro study.Webb CH, Hogg GMJ Hosp Infect10.1016/0195-6701(95)90069-11995Bacteria/*isolation & purification, Catheterization/*instrumentation, *Equipment Contamination, Equipment Design, Humans, Intubation, Intratracheal, Respiration, Artificial, Suction/*instrumentationPathogenicityEnzymology
9096179Sensitivity testing of ciprofloxacin for Pseudomonas aeruginosa.Ibrahim-Elmagboul IB, Livermore DMJ Antimicrob Chemother10.1093/jac/39.3.3091997Ciprofloxacin/*pharmacology, Drug Resistance, Microbial, Microbial Sensitivity Tests/methods, Pseudomonas aeruginosa/*drug effects, Regression AnalysisPathogenicity
10381102Quinolone accumulation by Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli.Piddock LJ, Jin YF, Ricci V, Asuquo AEJ Antimicrob Chemother10.1093/jac/43.1.611999Anti-Infective Agents/*analysis/pharmacokinetics, Carbonyl Cyanide m-Chlorophenyl Hydrazone/analysis/pharmacokinetics, Ciprofloxacin/pharmacokinetics, Diffusion, Dose-Response Relationship, Drug, Enoxacin/pharmacokinetics, Enrofloxacin, Escherichia coli/*chemistry, Fluorescence, *Fluoroquinolones, Hydrogen-Ion Concentration, Microbial Sensitivity Tests, Nalidixic Acid/analysis/pharmacokinetics, Pseudomonas aeruginosa/*chemistry, Quinolones/pharmacokinetics, Spheroplasts/metabolism, Staphylococcus aureus/*chemistry, TemperatureEnzymologyMetabolism
12680622In vitro antibacterial activity of Omani and African honey.Al-Jabri AA, Nzeako B, Al Mahrooqi Z, Al Naqdy A, Nsanze HBr J Biomed Sci10.1080/09674845.2003.117836682003Africa, Anti-Bacterial Agents/*pharmacology, Bacteria/*drug effects, Escherichia coli/drug effects, *Honey, Oman, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effectsPathogenicity
15254026Tolerance of Pseudomonas aeruginosa to Melaleuca alternifolia (tea tree) oil is associated with the outer membrane and energy-dependent cellular processes.Longbottom CJ, Carson CF, Hammer KA, Mee BJ, Riley TVJ Antimicrob Chemother10.1093/jac/dkh3592004Anti-Infective Agents/pharmacology, Anti-Infective Agents, Local/*pharmacology, Bacterial Outer Membrane Proteins/*drug effects/*metabolism, Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology, Drug Resistance, Bacterial, Edetic Acid/pharmacology, Energy Metabolism/*drug effects, Microscopy, Electron, Polymyxin B/*analogs & derivatives/pharmacology, Pseudomonas aeruginosa/*drug effects/ultrastructure, Tea Tree Oil/*pharmacology, Time Factors, Uncoupling Agents/pharmacologyPathogenicityMetabolism
15649007Evaluation of the neutralising capacity of bactec medium for some antibiotics.Nzeako BC, Al-Qasabi SSBr J Biomed Sci10.1080/09674845.2004.117326652004Amikacin/pharmacology, Amoxicillin/pharmacology, Amphotericin B/pharmacology, Anti-Bacterial Agents/*pharmacology, Bacteria/drug effects, Chloramphenicol/pharmacology, Culture Media, Gentamicins/pharmacology, Penicillins/pharmacology, Vancomycin/pharmacologyCultivationPathogenicity
18955267In vitro Evaluation of the Antiviral Activity of an Extract of Date Palm (Phoenix dactylifera L.) Pits on a Pseudomonas Phage.Jassim SA, Naji MAEvid Based Complement Alternat Med10.1093/ecam/nem1602007
20040963Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India.Javiya VA, Ghatak SB, Patel KR, Patel JAIndian J Pharmacol10.4103/0253-7613.441562008
21618272Synthesis and in-vitro antimicrobial activity of secondary and tertiary amines containing 2-chloro-6-methylquinoline moiety.Kumar S, Bawa S, Kaushik D, Panda BPArch Pharm (Weinheim)10.1002/ardp.2010003522011Amines/chemical synthesis/chemistry/*pharmacology, Anti-Bacterial Agents/chemical synthesis/chemistry/*pharmacology, Antifungal Agents/chemical synthesis/chemistry/*pharmacology, Bacteria/drug effects, Fungi/drug effects, Magnetic Resonance Spectroscopy/methods, Mass Spectrometry, Microbial Sensitivity Tests, Quinolines/chemical synthesis/chemistry/*pharmacology, Spectrophotometry, InfraredPathogenicity
23057159Exposure to clinical X-ray radiation does not alter antibiotic susceptibility or genotype profile in gram-negative and gram-positive clinical pathogens.Hayashi K, Hirayama J, Goldsmith CE, Coulter WA, Millar BC, Dooley JS, Loughrey A, Rooney PJ, Matsuda M, Moore JEBr J Biomed Sci2012Anti-Bacterial Agents/*pharmacology, Bacteria/drug effects/*genetics/*radiation effects, DNA, Bacterial/*genetics/*radiation effects, Dose-Response Relationship, Radiation, Drug Resistance, Bacterial/*genetics/*radiation effects, Genotype, Mutation/genetics/radiation effects, Radiation DosageGeneticsPathogenicity
23333146Integrity of bacterial genomic DNA after autoclaving: possible implications for horizontal gene transfer and clinical waste management.Yap JM, Goldsmith CE, Moore JEJ Hosp Infect10.1016/j.jhin.2012.11.0162013DNA, Bacterial/genetics/*radiation effects, Escherichia coli/*genetics/radiation effects, Gene Transfer, Horizontal/*radiation effects, Hot Temperature, Humans, Medical Waste Disposal/*methods, Polymerase Chain Reaction, Pseudomonas aeruginosa/*genetics/radiation effects, Salmonella/*genetics/radiation effects, Sterilization/*methods, Time FactorsStressPathogenicity
23569753Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa.Habbal O, Hasson SS, El-Hag AH, Al-Mahrooqi Z, Al-Hashmi N, Al-Bimani Z, Al-Balushi MS, Al-Jabri AAAsian Pac J Trop Biomed10.1016/S2221-1691(11)60021-X2011Anti-Bacterial Agents/*pharmacology, Bacteria/drug effects, Lawsonia Plant/*chemistry, Microbial Sensitivity Tests, Plant Components, Aerial/chemistry, Plant Extracts/*pharmacology, Pseudomonas aeruginosa/*drug effectsPathogenicity
23856977Convenient syntheses of cyanuric chloride-derived NHC ligands, their Ag(I) and Au(I) complexes and antimicrobial activity.Almalioti F, MacDougall J, Hughes S, Hasson MM, Jenkins RL, Ward BD, Tizzard GJ, Coles SJ, Williams DW, Bamford S, Fallis IA, Dervisi ADalton Trans10.1039/c3dt51400e2013Anti-Infective Agents/*chemical synthesis/chemistry/pharmacology, Bacteria/drug effects, Candida albicans/drug effects, Coordination Complexes/*chemical synthesis/chemistry/pharmacology, Gold/*chemistry, Heterocyclic Compounds/chemistry, Imidazoles/chemistry, Ligands, Methane/*analogs & derivatives/chemistry, Silver/*chemistry, Triazines/*chemistryPathogenicity
24361354Evaluation of Galleria mellonella larvae for measuring the efficacy and pharmacokinetics of antibiotic therapies against Pseudomonas aeruginosa infection.Hill L, Veli N, Coote PJInt J Antimicrob Agents10.1016/j.ijantimicag.2013.11.0012013Animals, Anti-Bacterial Agents/*pharmacokinetics/*therapeutic use, *Disease Models, Animal, Hemolymph/chemistry, Humans, Larva/microbiology, Lepidoptera/*microbiology, Pseudomonas Infections/*drug therapy, Pseudomonas aeruginosa/*drug effects/*growth & development, Survival Analysis, Treatment OutcomePathogenicityEnzymology
26256176Development of bio-composites with novel characteristics: Evaluation of phenol-induced antibacterial, biocompatible and biodegradable behaviours.Iqbal HM, Kyazze G, Locke IC, Tron T, Keshavarz TCarbohydr Polym10.1016/j.carbpol.2015.05.0462015Anti-Bacterial Agents/*pharmacology, Bacteria/drug effects, Biocompatible Materials/*pharmacology, Biodegradation, Environmental/drug effects, Cell Adhesion/drug effects, Cell Line, Cell Shape/drug effects, Cell Survival/drug effects, Gallic Acid/chemistry, Humans, Microbial Sensitivity Tests, Phenol/*pharmacology, Soil, Spectroscopy, Fourier Transform InfraredPathogenicity
29654885Fabrication of inhaled hybrid silver/ciprofloxacin nanoparticles with synergetic effect against Pseudomonas aeruginosa.Al-Obaidi H, Kalgudi R, Zariwala MGEur J Pharm Biopharm10.1016/j.ejpb.2018.04.0062018Administration, Inhalation, Anti-Bacterial Agents/*pharmacology/therapeutic use, Biofilms/drug effects, Chemical Engineering/methods, Ciprofloxacin/pharmacology/therapeutic use, Cystic Fibrosis/*complications, Drug Compounding/*methods, Drug Synergism, Humans, Metal Nanoparticles/chemistry, Microbial Sensitivity Tests, Pseudomonas Infections/*drug therapy/etiology, Pseudomonas aeruginosa/*drug effects/physiology, Silicon Dioxide/chemistry, Silver/pharmacology/therapeutic usePathogenicity
31349506Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity.De-Paula MMM, Afewerki S, Viana BC, Webster TJ, Lobo AO, Marciano FRMater Sci Eng C Mater Biol Appl10.1016/j.msec.2019.1097782019Cell Differentiation/*drug effects, Cell Line, *Histones/chemistry/pharmacology, Humans, *Intercellular Signaling Peptides and Proteins/chemistry/pharmacology, Methicillin-Resistant Staphylococcus aureus/*growth & development, Osteoblasts/*metabolism, Pseudomonas aeruginosa/*growth & development, *Tissue Engineering, Tissue Scaffolds/*chemistryPathogenicityMetabolism
31692847Isolation and screening of actinomycetes producing antimicrobial substances from an extreme Moroccan biotope.El Karkouri A, Assou SA, El Hassouni MPan Afr Med J10.11604/pamj.2019.33.329.190182019Actinobacteria/isolation & purification/*metabolism, Anti-Infective Agents/isolation & purification/*pharmacology, Bacteria/*drug effects, Candida albicans/drug effects, Culture Media, Microbial Sensitivity Tests, Morocco, *Soil Microbiology, TemperaturePhylogenyEnzymology
32245064Legionella Detection in Water Networks as per ISO 11731:2017: Can Different Filter Pore Sizes and Direct Placement on Culture Media Influence Laboratory Results?De Giglio O, Diella G, Trerotoli P, Consonni M, Palermo R, Tesauro M, Lagana P, Serio G, Montagna MTInt J Environ Res Public Health10.3390/ijerph170620772020Culture Media, Escherichia coli/isolation & purification, Humans, *Legionella pneumophila/isolation & purification, Legionnaires' Disease/epidemiology, Water, *Water Microbiology, Water SupplyCultivationEnzymology
34497647Chemical and Antimicrobial Analyses of Juniperus chinensis and Juniperus seravschanica Essential Oils and Comparison with Their Methanolic Crude Extracts.Khamis AS, Chai LCInt J Anal Chem10.1155/2021/99375222021
36109567Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection.Nesa J, Jana SK, Sadat A, Biswas K, Kati A, Kaya O, Mondal R, Dam P, Thakur M, Kumar A, Hossain M, Lima LR, Rezende SB, Bhattacharjya D, Gangopadhyay D, Ghorai S, Altuntas S, Panda AK, Chakrabarti P, Swarnakar S, Chakraborty J, Yilmaz B, Macedo MLR, Franco OL, Cardoso MH, Mandal AKSci Rep10.1038/s41598-022-19450-82022Adenosine Monophosphate, Animals, *Ant Venoms, Anti-Bacterial Agents/pharmacology, *Anti-Infective Agents, *Bombyx, Hemolymph, Humans, Methanol, Peptides/chemistry, *Pseudomonas Infections/drug therapy, WaterPathogenicity

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
32781Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103837Collection of Institut Pasteur (CIP 103837)
44370Curators of the CCUGhttps://www.ccug.se/strain?id=1423Culture Collection University of Gothenburg (CCUG) (CCUG 1423)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
91977Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID376990.1