Strain identifier

BacDive ID: 1344

Type strain: Yes

Species: Halobacillus karajensis

Strain Designation: X78, MA-2, MA-2T

Strain history: CIP <- 2004, DSMZ <- F. Malekzadeh <- M. A. Amoozegar, Tehran Univ., Tehran, Iran: strain MA-2T

NCBI tax ID(s): 195088 (species)

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General

@ref: 5615

BacDive-ID: 1344

DSM-Number: 14948

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, colony-forming, pigmented

description: Halobacillus karajensis X78 is an obligate aerobe, spore-forming, mesophilic bacterium that forms circular colonies and has a white pigmentation.

NCBI tax id

  • NCBI tax id: 195088
  • Matching level: species

strain history

@refhistory
5615<- F. Malekzadeh; MA-2 <- M. A. Amoozegar
120391CIP <- 2004, DSMZ <- F. Malekzadeh <- M. A. Amoozegar, Tehran Univ., Tehran, Iran: strain MA-2T

doi: 10.13145/bacdive1344.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halobacillus
  • species: Halobacillus karajensis
  • full scientific name: Halobacillus karajensis Amoozegar et al. 2003

@ref: 5615

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Halobacillus

species: Halobacillus karajensis

full scientific name: Halobacillus karajensis Amoozegar et al. 2003

strain designation: X78, MA-2, MA-2T

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
22984positive2.5-4.0 µm0.8-0.9 µmrod-shapedno
69480yes96.115
69480positive100
120391positiverod-shapedno

colony morphology

  • @ref: 22984
  • colony size: 2.0 mm
  • colony color: cream or white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: nutrient agar plus 10 % (w/v) NaCl

pigmentation

  • @ref: 22984
  • production: yes
  • color: white

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5615NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
22984nutrient agar plus 10 % (w/v) NaClyes
38271Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120391CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5615positivegrowth38mesophilic
22984positivegrowth10.0-49.0
22984positiveoptimum34.0-38.0mesophilic
38271positivegrowth37mesophilic
120391positivegrowth15-41
120391nogrowth5psychrophilic
120391nogrowth10psychrophilic

culture pH

@refabilitytypepHPH range
22984positivegrowth6.0-9.6alkaliphile
22984positiveoptimum7.5-8.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22984obligate aerobe
120391facultative anaerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
22984spherical or ellipsoidal endospores,central or subterminal positionendosporeyes
69481yes100
69480yes100
120391yes

compound production

  • @ref: 5615
  • compound: amylase

halophily

@refsaltgrowthtested relationconcentration
22984NaClpositivegrowth1.0-24.0 %(w/v)
22984NaCloptimum10.0 %(w/v)
120391NaClpositivegrowth2-6 %
120391NaClnogrowth0 %
120391NaClnogrowth8 %
120391NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2298416947citrate-carbon source
2298427897tryptophan-energy source
2298417234glucose-fermentation
2298416947citrate-growth
2298417924D-sorbitol-growth
2298415443inulin-growth
2298417814salicin-growth
2298417108D-arabinose-hydrolysis
2298412936D-galactose-hydrolysis
2298465327D-xylose-hydrolysis
2298417992sucrose-hydrolysis
2298453424tween 20-hydrolysis
2298453426tween 80-hydrolysis
2298416199urea-hydrolysis
2298415824D-fructose+builds acid from
2298417634D-glucose+builds acid from
2298417306maltose+builds acid from
2298429864mannitol+builds acid from
2298437684mannose+builds acid from
2298416634raffinose+builds acid from
2298430089acetate+carbon source
2298417057cellobiose+carbon source
2298417634D-glucose+carbon source
2298414314D-glucose 6-phosphate+carbon source
2298428053melibiose+carbon source
2298423652dextrin+carbon source
2298417306maltose+carbon source
2298417268myo-inositol+carbon source
2298417272propionate+carbon source
2298428017starch+carbon source
2298430031succinate+carbon source
2298430089acetate+energy source
2298417057cellobiose+energy source
2298417634D-glucose+energy source
2298414314D-glucose 6-phosphate+energy source
2298428053melibiose+energy source
2298423652dextrin+energy source
2298417306maltose+energy source
2298417268myo-inositol+energy source
2298417272propionate+energy source
2298428017starch+energy source
2298430031succinate+energy source
22984casein+hydrolysis
2298416991dna+hydrolysis
229844853esculin+hydrolysis
229845291gelatin+hydrolysis
2298428017starch+hydrolysis
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
12039116947citrate-carbon source
1203914853esculin-hydrolysis
12039117234glucose-fermentation
12039117716lactose-fermentation
12039117632nitrate-reduction
12039116301nitrite-reduction
120391132112sodium thiosulfate-builds gas from
12039117632nitrate+respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2298417076streptomycinyesyes10 µg
2298448923erythromycinyesyes15 µg
2298418208penicillin gyesyes10 Unit
2298428971ampicillinyesyes10 µg
229842637amikacinyesyes30 µg
22984124991cefalotinyesyes30 µg
2298417698chloramphenicolyesyes30 µg
22984100147nalidixic acidyesyes30 µg
2298427902tetracyclineyesyes30 µg
2298428077rifampicinyesyes5 µg

metabolite production

@refChebi-IDmetaboliteproduction
2298435581indoleno
2298416136hydrogen sulfideno
2298415688acetoinno
12039135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole testcitrate test
2298415688acetoin-
2298417234glucose-
2298435581indole-
2298416947citrate-
12039115688acetoin-
12039117234glucose-

enzymes

@refvalueactivityec
22984catalase+1.11.1.6
22984cytochrome oxidase+1.9.3.1
22984phenylalanine deaminase-4.3.1.5
22984tryptophan deaminase-4.1.99.1
22984urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120391oxidase+
120391beta-galactosidase-3.2.1.23
120391alcohol dehydrogenase-1.1.1.1
120391gelatinase+
120391amylase+
120391DNase+
120391caseinase-3.4.21.50
120391catalase+1.11.1.6
120391tween esterase-
120391gamma-glutamyltransferase-2.3.2.2
120391lecithinase-
120391lipase-
120391lysine decarboxylase-4.1.1.18
120391ornithine decarboxylase-4.1.1.17
120391phenylalanine ammonia-lyase-4.3.1.24
120391tryptophan deaminase-
120391urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120391-+++--+----+--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120391+--+++----++----++---+/------+--++---++--+-+----+-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120391++-+--+++++----+-+-++++-+-+-+--++++++--+---+-+-+++++++++++-++++++------+++++---++++++-+-+++++++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture temperature
22984surface saline soilKaraj regionIranIRNAsiabroth mediumNaCl: 81 g l-1; MgCl2.6H2O: 7 g l-1; MgSO4.7H2O 9.7 g l-1; CaCl2 0.36 g l-1; KCl 2 g l-1 ;NaHCO3 0.06 g l-1; NaBr 0.026 g l-1(total salts,100 g l-1)34.00
5615saline soilKaradjIranIRNAsia
120391Environment, Saline soilIranIRNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_774.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_469;97_536;98_617;99_774&stattab=map
  • Last taxonomy: Halobacillus
  • 16S sequence: AJ486874
  • Sequence Identity:
  • Total samples: 5980
  • soil counts: 2306
  • aquatic counts: 2677
  • animal counts: 590
  • plant counts: 407

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56151Risk group (German classification)
1203911Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halobacillus karajensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 459AB367154459ena195088
20218Halobacillus karajensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 703AB367155703ena195088
22984Halobacillus karajiensis partial 16S rRNA gene, type strain DSM 14948AJ4868741540nuccore195088

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halobacillus karajensis DSM 14948GCA_900108515scaffoldncbi195088
66792Halobacillus karajensis strain DSM 14948195088.4wgspatric195088
66792Halobacillus karajensis DSM 149482651870103draftimg195088

GC content

  • @ref: 22984
  • GC-content: 41.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
glucose-fermentno90.037no
motileyes85.733yes
flagellatedyes84.441yes
gram-positiveyes91.743yes
anaerobicno99.16yes
aerobicyes96.046yes
halophileyes93.342no
spore-formingyes96.289yes
thermophileno97.07no
glucose-utilyes89.665yes

External links

@ref: 5615

culture collection no.: DSM 14948, CIP 108359, LMG 21515

straininfo link

  • @ref: 70994
  • straininfo: 87983

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12892126Halobacillus karajensis sp. nov., a novel moderate halophile.Amoozegar MA, Malekzadeh F, Malik KA, Schumann P, Sproer CInt J Syst Evol Microbiol10.1099/ijs.0.02448-02003Bacillaceae/*classification/genetics/isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Iran, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil MicrobiologyGenetics
Enzymology18781348Production, optimization and purification of a novel extracellular protease from the moderately halophilic bacterium Halobacillus karajensis.Karbalaei-Heidari HR, Amoozegar MA, Hajighasemi M, Ziaee AA, Ventosa AJ Ind Microbiol Biotechnol10.1007/s10295-008-0466-y2008Bacillaceae/chemistry/*enzymology/isolation & purification/metabolism, Bacterial Proteins/chemistry/*isolation & purification/metabolism, Culture Media/chemistry/metabolism, *Culture Techniques, Enzyme Stability, Extracellular Space/chemistry/*enzymology, Kinetics, Molecular Weight, Peptide Hydrolases/chemistry/*isolation & purification/metabolism, Sodium Chloride/*metabolism, Soil MicrobiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5615Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14948)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14948
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22984M. A. Amoozegar,F. Malekzadeh,K. A. Malik,P. Schumann,C. Spröer10.1099/ijs.0.02448-0Halobacillus karajensis sp. nov., a novel moderate halophileIJSEM 53: 1059-1063 200312892126
38271Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5972
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70994Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87983.1StrainInfo: A central database for resolving microbial strain identifiers
120391Curators of the CIPCollection of Institut Pasteur (CIP 108359)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108359